An integrated microfluidic device for DNA purification and PCR amplification of STR fragments

Rapid microfluidic solid-phase extraction system for hyper-methylated DNA enrichment and epigenetic analysis

“…[12][13][14][15] There have been many reports of lSPE DNA extraction. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] Previous reports describe DNA lSPE systems with integrated polymerase chain reaction 21,22 and electrophoretic separation. [23][24][25] Furthermore, DNA lSPE has been applied to forensics, where samples are typically very dilute.…”

A protocol for direct and rapid multiplex PCR amplification on forensically relevant samples

“…Especially for this latter purpose, the time available for generating DNA information may be limited, for instance in cases of crimes by a serial perpetrator with a high risk of repetition, abduction with danger of life, time-restricted threats against persons or places, or when custody time of suspects is limited for legal reasons. Several approaches can reduce DNA profiling time: (1) the use of a rapid amplification protocol [1][2][3][4]; (2) DNA profiling without prior DNA extraction, directly on a biological stain [5][6][7] or (3) DNA typing at the scene of crime or police station via automated, miniaturized devices [8][9][10]. The first two approaches have the advantage that DNA typing is performed under the strict laboratory conditions befitting forensic casework, and that amplified products are separated at the nucleotide-level on validated genetic analyzers.…”

Forensic DNA Analysis

“…There has been progress on microfluidic devices ('lab on a chip') since this was first discussed in forensics over a decade ago (Horsman et al 2007). So far work on three more or less integrated platforms has been published (Giese et al 2009;Bienvenue et al 2010;Hopwood et al 2010;Hurth et al 2010;Liu et al 2011), with at least one more instrument under development by the company IntegenX (Butler 2011). The goal for a typical integrated system is to generate STR results for several samples in parallel in less than 3 hours.…”