An integrated resource for functional and structural connectivity of the marmoset brain

Abstract

Comprehensive integration of structural and functional connectivity data is required to model brain functions accurately. While resources for studying the structural connectivity of non-human primate brains already exist, their integration with functional connectivity data has remained unavailable. Here we present a comprehensive resource that integrates the most extensive awake marmoset resting-state fMRI data available to date (39 marmoset monkeys, 710 runs, 12117 mins) with previously published cellular-level neuronal tracing data (52 marmoset monkeys, 143 injections) and multi-resolution diffusion MRI datasets. The combination of these data allowed us to (1) map the fine-detailed functional brain networks and cortical parcellations, (2) develop a deep-learning-based parcellation generator that preserves the topographical organization of functional connectivity and reflects individual variabilities, and (3) investigate the structural basis underlying functional connectivity by computational modeling. This resource will enable modeling structure-function relationships and facilitate future comparative and translational studies of primate brains.

Marmoset Brain Mapping V5: an Anatomical and Connectivity Atlas of the Cerebellum

“…5A), highlighting the importance of these areas for cerebello-cerebral communications. To verify the pattern, we calculated the variation of correlations to all fifteen networks that were defined in our recent study 15 for each cerebellar vertex and obtained a map of variation (Fig. 5B), where low values represented consistent connectivity across different networks and vice versa.…”

“…The above gradient analyses showed the topological patterns of the cerebello-cerebral functional connectivity but did not demonstrate which cerebral regions connected with the cerebellum. In our previous studies, we provided a comprehensive mapping of the functional networks of the marmoset cerebral cortex 15 . Here, we calculated the functional connectivity of each cerebellar vertex to these cerebral networks to obtain the cerebellar connectivity maps of each network (Fig.…”

“…Additionally, in comparison to marmosets, the functional connectivity between the cerebellum and the cerebral cortex was stronger in humans, supporting the notion that the intra-cerebellar gradients and cerebello-cerebral gradients are more similar in humans due to stronger connections between the cerebral cortex and cerebellar cortex. The cerebral networks were defined by our previous study 15 . DMN: default model network.…”

“…A detailed scanning protocol was described in the MBMv2 resource paper 13 . The awake resting-state fMRI data, from version 4 (MBMv4) of the Marmoset Brain Mapping project 15 , were used to examine the functional connectivity and gradient patterns of the cerebellum. The data was collected from 39 marmosets, which were scanned repeatedly and resulted in a total of 710 fMRI runs (17 min per run).…”

“…All of which has enabled the development of valuable open resources and research tools [4][5][6][7][8][9][10][11] . Specifically, via the Marmoset Brain Mapping project (marmosetbrainmapping.org), we have provided ultra-high-resolution ex-vivo diffusion MRI and awake resting-state fMRI data of the marmoset brain [12][13][14][15] . Based on the resource, we have produced an anatomical and functional atlas of the marmoset cerebral cortex in fine detail and created useful templates for surface-based visualization and analysis.…”