An integrated rotary microfluidic system with DNA extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics

Park, Byung Hyun; Oh, Seung Jun; Jung, Jae Hwan; Choi, Goro; Seo, Ji Hyun; Kim, Do Hyun; Lee, Eun Yeol; Seo, Tae Seok

doi:10.1016/j.bios.2016.11.063

Cited by 208 publications

(91 citation statements)

References 40 publications

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“…To the best of our knowledge this is the first study combining AuNPs, LAMP and microfluidics, which has undergone an extensive evaluation in food samples (LoD, SE, SP, AC, PPV, NPV, k ), being critical for future implementation in the food industry. Previous studies have been just focused on the development of the methodology, but a thorough evaluation of its applicability in food products was missing ( Fang et al, 2010a , b ; Wong et al, 2014 ; Sayad et al, 2016 ; Park et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Combination of Microfluidic Loop-Mediated Isothermal Amplification with Gold Nanoparticles for Rapid Detection of Salmonella spp. in Food Samples

et al. 2017

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Foodborne diseases are an important cause of morbidity and mortality. According to the World Health Organization, there are 31 main global hazards, which caused in 2010 600 million foodborne illnesses and 420000 deaths. Among them, Salmonella spp. is one of the most important human pathogens, accounting for more than 90000 cases in Europe and even more in the United States per year. In the current study we report the development, and thorough evaluation in food samples, of a microfluidic system combining loop-mediated isothermal amplification with gold nanoparticles (AuNPs). This system is intended for low-cost, in situ, detection of different pathogens, as the proposed methodology can be extrapolated to different microorganisms. A very low limit of detection (10 cfu/25 g) was obtained. Furthermore, the evaluation of spiked food samples (chicken, turkey, egg products), completely matched the expected results, as denoted by the index kappa of concordance (value of 1.00). The results obtained for the relative sensitivity, specificity and accuracy were of 100% as well as the positive and negative predictive values.

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Section: Discussionmentioning

confidence: 99%

Combination of Microfluidic Loop-Mediated Isothermal Amplification with Gold Nanoparticles for Rapid Detection of Salmonella spp. in Food Samples

et al. 2017

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“…Compared with the previous design, the LAMP disk system was proposed, integrated with DNA extraction and amplicon detection using a colorimetric lateral flow strip ( Fig. 2D) [46]. It consisted of three major microfluidic layers from top to bottom for solution loading and transportation, DNA extraction and amplification, and lateral flow strip detection, respectively.…”

Section: Multi-gene Detectionmentioning

confidence: 99%

LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification

Zhang

Fohlerová

et al. 2019

TrAC Trends in Analytical Chemistry

186

126

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a b s t r a c tNucleic acid amplification for the detection of infectious diseases, food pathogens, or assessment of genetic disorders require a laboratory setting with specialized equipment and technical expertise. Isothermal deoxyribonucleic acid amplification methods, such as loop-mediated isothermal amplification (LAMP), exhibit characteristics ideal for point-of-care (POC) applications, since their instrumentation is simpler in comparison with the standard method of polymerase chain reaction. Other key advantages of LAMP are robustness and the production of pyrophosphate in the presence of the target gene, enabling to detect the reaction products using the naked eye. Polymerase inhibitors, presented in clinical samples, do not affect the amplification process, making LAMP suitable for a simple sample-to-answer diagnostic systems with simplified sample preparation. In this review, we discuss the trends in miniaturized LAMP techniques, such as microfluidic, paper-based, and digital with their advantages and disadvantages, especially for POC applications alongside our opinion of the future development of miniaturized LAMP.

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“…The MSB with a concentration of 100 mg/mL were then pumped to fill the coaxial channel at the absence of the magnetic field and distributed uniformly in the channel when the magnetic field was applied. After the lysed bacteria sample was pumped into the coaxial channel, the DNA was captured by the MSB through electrostatic interaction at the presence of chaotropic salts (Park et al, 2017), and 1 mL of absolute ethanol was pumped to remove the residual proteins. Finally, 100 µL of the deionized water was pumped to fill the coaxial channel and the pump was automatically controlled by a microcontroller to work clockwise for 3 s and anti-clockwise for 3 s for 20 min for eluting the DNA from the MSB to obtain the purified and concentrated DNA templates.…”

Section: Extraction Of the Target Dnamentioning

confidence: 99%

Rapid and sensitive detection of Escherichia coli O157:H7 using coaxial channel-based DNA extraction and microfluidic PCR

Zhang

Huang

Cai

et al. 2018

Journal of Dairy Science

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In this study, a rapid and sensitive method for detection of Escherichia coli O157:H7 using the coaxial channel-based DNA extraction and the microfluidic PCR was proposed and verified. The magnetic silica beads were first pumped into the coaxial channel, which was captured in the coaxial channel more uniformly by applying the multiring high-gradient magnetic field. After the E. coli O157:H7 cells were lysed with the lysis buffer to release the DNA, the improved coaxial channel was used to efficiently extract the DNA, followed by washing with ethanol to remove the residual proteins and eluting with a small volume of deionized water to obtain the purified and concentrated DNA. Finally, the obtained DNA was amplified and determined using the microfluidic PCR. This proposed bacteria detection method was able to detect E. coli O157:H7 as low as 12 cfu/mL when the large volume (10 mL) of bacterial sample was used, and the recovery of E. coli O157:H7 in the spiked milk samples ranged from 97.4 to 100.6%. This proposed bacteria detection method has shown great potential to detect lower concentration of E. coli O157:H7 from larger volumes of sample.

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An integrated rotary microfluidic system with DNA extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics

Cited by 208 publications

References 40 publications

Combination of Microfluidic Loop-Mediated Isothermal Amplification with Gold Nanoparticles for Rapid Detection of Salmonella spp. in Food Samples

Combination of Microfluidic Loop-Mediated Isothermal Amplification with Gold Nanoparticles for Rapid Detection of Salmonella spp. in Food Samples

LAMP-on-a-chip: Revising microfluidic platforms for loop-mediated DNA amplification

Rapid and sensitive detection of Escherichia coli O157:H7 using coaxial channel-based DNA extraction and microfluidic PCR

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