An integrated superhydrophobic-plasmonic biosensor for mid-infrared protein detection at the femtomole level

Ninno, Adele De; Ciasca, Gabriele; Gerardino, A.; Calandrini, Eugenio; Papi, Massimiliano; Spirito, Marco De; Nucara, A.; Ortolani, Michele; Businaro, Luca; Baldassarre, Leonetta

doi:10.1039/c4cp05023a

Cited by 30 publications

(38 citation statements)

References 28 publications

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“…This technique overcomes these limitations, and the authors suggest its utility for detecting minute concentrations of cancer biomarkers. A similar approach is described by De Ninno et al [198], where an array of Cr/Au nanorods are fabricated surrounded by a region of hydrophilic polymer, further surrounded by superhydrophobic PMMA pillars. This pattern confined the aqueous droplets to the region during evaporation, concentrating the protein on the nanorods for plasmonic detection.…”

Section: Diagnostic Applicationsmentioning

confidence: 99%

Superhydrophobic materials for biomedical applications

et al. 2016

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Superhydrophobic surfaces are actively studied across a wide range of applications and industries, and are now finding increased use in the biomedical arena as substrates to control protein adsorption, cellular interaction, and bacterial growth, as well as platforms for drug delivery devices and for diagnostic tools. The commonality in the design of these materials is to create a stable or metastable air state at the material surface, which lends itself to a number of unique properties. These activities are catalyzing the development of new materials, applications, and fabrication techniques, as well as collaborations across material science, chemistry, engineering, and medicine given the interdisciplinary nature of this work. The review begins with a discussion of superhydrophobicity, and then explores biomedical applications that are utilizing superhydrophobicity in depth including material selection characteristics, in vitro performance, and in vivo performance. General trends are offered for each application in addition to discussion of conflicting data in the literature, and the review concludes with the authors’ future perspectives on the utility of superhydrophobic surfaces for biomedical applications.

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Section: Diagnostic Applicationsmentioning

confidence: 99%

Superhydrophobic materials for biomedical applications

et al. 2016

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“…These technologies are capable of rapid, portable, accurate, and inexpensive detection of biomarkers. For example, nanomaterial-enhanced surface plasmon resonance or nanoplasmonic sensors (based on optical spectroscopy) are able to detect molecules at femtomole (or even lower) concentrations (Ninno et al, 2015). The multiplexing capacity of microfluidics offers the potential for improving sensitivity and specificity by combining several markers for analysis into a single device.…”

Section: New Detection Technologiesmentioning

confidence: 99%

Advances in the microbial etiology and pathogenesis of early childhood caries

Hajishengallis

Parsaei

Klein

et al. 2016

Molecular Oral Microbiology

207

187

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Early childhood caries (ECC) is one of the most prevalent infectious diseases affecting children worldwide. ECC is an aggressive form of dental caries, which left untreated, can result in rapid and extensive cavitation in teeth (rampant caries) that is painful and costly to treat. Furthermore, it affects mostly children from impoverished background, and thus constitutes a major challenge in public health. The disease is a prime example of the consequences arising from complex, dynamic interactions between microorganisms, host and diet, leading to the establishment of highly pathogenic (cariogenic) biofilms. To date, there are no effective methods to identify those at risk of developing ECC or control the disease in affected children. Recent advances in deep-sequencing technologies, novel imaging methods and (meta)proteomics-metabolomics approaches provide an unparalleled potential to reveal new insights to illuminate our current understanding about the etiology and pathogenesis of the disease. In this concise review, we provide a broader perspective about the etiology and pathogenesis of ECC based on previous and current knowledge on biofilm matrix, microbial diversity and host-microbe interactions which could have direct implications for developing new approaches for improved risk assessment and prevention of this devastating and costly childhood health condition.

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“…The surface can be nanostructured or chemically modified to enrich the analyte in the range of the evanescent wave . For the secondary structure analysis of a specific protein within a complex body fluid, the protein of interest has to be selectively and specifically bound to the surface.…”

Section: Introductionmentioning

confidence: 99%

An infrared sensor analysing label-free the secondary structure of the Abeta peptide in presence of complex fluids

et al. 2015

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The secondary structure change of the Abeta peptide to beta‐sheet was proposed as an early event in Alzheimer's disease. The transition may be used for diagnostics of this disease in an early state. We present an Attenuated Total Reflection (ATR) sensor modified with a specific antibody to extract minute amounts of Abeta peptide out of a complex fluid. Thereby, the Abeta peptide secondary structure was determined in its physiological aqueous environment by FTIR‐difference‐spectroscopy. The presented results open the door for label‐free Alzheimer diagnostics in cerebrospinal fluid or blood. It can be extended to further neurodegenerative diseases.

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An integrated superhydrophobic-plasmonic biosensor for mid-infrared protein detection at the femtomole level

Cited by 30 publications

References 28 publications

Superhydrophobic materials for biomedical applications

Superhydrophobic materials for biomedical applications

Advances in the microbial etiology and pathogenesis of early childhood caries

An infrared sensor analysing label-free the secondary structure of the Abeta peptide in presence of complex fluids

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