Availability of micronutrients is one of the important factors for the successful and economic cultivation of vineyards. The objectives of this study are to evaluate the spatial distribution of soil micronutrients (Cu, Fe, Mn, Zn, B) and their suitability for vineyard cultivation in Jabal Al Arab of Syria. To achieve the research objectives, soil samples were collected randomly from vineyard farms which cover the study area. Following this, soil analyses were conducted to determine the concentration of soil micronutrients. Results showed that soil micronutrient concentrations did not reach the minimum threshold for vineyard cultivation with 1.1, 12.06, 11.2, 2.6 and 0.3 ppm for Cu, Fe, Mn, Zn and B, respectively. Furthermore, spatial distribution showed that 63%, 39%, 34%, 76% and 74% of the study area was affected by severe deficiency of Cu, Fe, Mn, Zn, B respectively.