An integrative process for obtaining lipids and glucose from Chlorella vulgaris biomass with a single treatment of cell disruption

Heo, Young Mok; Lee, Hanbyul; Lee, Changsu; Kang, Juwon; Ahn, Joon-Woo; Lee, Young Min; Kang, Kyu Young; Choi, Yoon E.; Kim, Jae Jin

doi:10.1016/j.algal.2017.09.022

Cited by 35 publications

(14 citation statements)

References 33 publications

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“…These results seem not to be in agreement with some other studies since sonication is commonly pointed as an inefficient disruption method [29,36]. For instance, the difference between the disruption efficiency obtained for sonication and microwave was reported as high as 27.2% by McMillan et al [29], which is clearly not the case in our results.…”

Section: Comparison Of Cell Disruption Techniques 321 Impact On Cellscontrasting

confidence: 95%

“…2), indicates a random effect over cells and it makes sense considering that the processing involving these three methodologies is not as constant and uniform as in the case of sonication and HSH. Heo et al [36] have verified the same behaviour applying microwave irradiation and sonication to C. vulgaris. According to those authors, cells' membranes were severely damaged when microwave was utilized, whereas sonication was responsible for cracking or tearing up the cells.…”

Section: Comparison Of Cell Disruption Techniques 321 Impact On Cellsmentioning

confidence: 69%

“…For instance, the difference between the disruption efficiency obtained for sonication and microwave was reported as high as 27.2% by McMillan et al [29], which is clearly not the case in our results. However, this disparity may be due to the species-dependency of disruption effectiveness once most of the studies use C. vulgaris [29,36], commonly considered a more robust and difficult-to-break microorganism.…”

Section: Comparison Of Cell Disruption Techniques 321 Impact On Cellsmentioning

confidence: 99%

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Evaluation of disruption/permeabilization methodologies for Microcystis aeruginosa as alternatives to obtain high yields of microcystin release

et al. 2019

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The extraction of the wide range of useful bioactive compounds produced by cyanobacteria is still a major bottleneck at industrial scale. In addition to the high costs, extraction efficiencies are also commonly low, with low cell disruption efficiencies playing a particularly significant role in intracellular compounds' release. To increase the chances of an extended use of the cyanobacteria toxin microcystin in several biotechnological fields, we aimed to optimize five different disruption techniques: bead milling, microwave, freeze-thaw cycles, highspeed homogenization, and sonication. For each of the methods tested, the conditions that maximized the intracellular organic matter release were: i) 20% of beads and treatment time of 7 min (bead milling); ii) 800 W for 1.5 min (microwave); iii) three 12-h freeze-thaw cycles at −20°C; iv) 15,000 rpm for 7 min (high-speed homogenization); and v) 40 kHz for 10 min (sonication). Sonication and freeze-thaw cycles followed by sonication revealed to be the most effective methodologies to ensure a maximum intracellular organic matter release and, consequently, microcystin availability for being extracted. The decrease of cells' viability was however more evident in freeze-thaw cycles, freeze-thaw cycles followed by sonication, and microwave where only 0.3, 0.05 and 0.9% of the initial cells, respectively, maintained their viability after being treated. On the other hand, sonication and bead milling reduced the viability of the original culture to 5 and 15.5%, respectively, while high-speed homogenization did not show any significant differences compared to control. According to the results obtained in this study, the most suitable methodology to maximize the release of microcystin was therefore the use of sonication (40 kHz) during 10 min.

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Section: Comparison Of Cell Disruption Techniques 321 Impact On Cellscontrasting

confidence: 95%

Section: Comparison Of Cell Disruption Techniques 321 Impact On Cellsmentioning

confidence: 69%

Section: Comparison Of Cell Disruption Techniques 321 Impact On Cellsmentioning

confidence: 99%

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Evaluation of disruption/permeabilization methodologies for Microcystis aeruginosa as alternatives to obtain high yields of microcystin release

et al. 2019

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“…This evidence was reinforced by a 7-fold augment of oligosaccharides content after the enzyme mixture treatment, as observed byHeo et al (2017). This evidence was reinforced by a 7-fold augment of oligosaccharides content after the enzyme mixture treatment, as observed byHeo et al (2017).…”

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confidence: 61%

A two‐enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets

Coelho

Lopes

Cardoso

et al. 2019

Animal Physiology Nutrition

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The main goal of this study was to test a rational combination of pre-selected carbohydrate-active enzymes (CAZymes) and sulphatases, individually or in combination, in order to evaluate its capacity to disrupt Arthrospira platensis cell wall, allowing the release of its valuable nutritional bioactive compounds. By the end, a two-enzyme constituted mixture (Mix), composed by a lysozyme and a α-amylase, was incubated with A. platensis suspension. The microalga cell wall disruption was evaluated through the amount of reducing sugars released from the cell wall complemented with the oligosaccharide profile by HPLC. An increase of the amount of reducing sugars up to 2.42 g/L in microalgae treated with the Mix relative to no treatment (p < .05), as well as a 7-fold increase of oligosaccharides amount (p < .001), were obtained. With resort of fluorescence microscopy, a 36% reduction of fluorescence intensity (p < .001) was observed using Calcofluor White staining. In the supernatant, the Mix caused a 1.34-fold increase in protein content (p = .018) relative to the control. Similarly, n-6 polyunsaturated fatty acids (PUFA) (p = .007), in particular 18:2n-6 (p = .016), monounsaturated fatty acids (MUFA) (p = .049) and chlorophyll a (p = .025) contents were higher in the supernatant of microalgae treated with the enzyme mixture in relation to the control. Taken together, these results point towards the disclosure of a novel two-enzyme mixture able to partial degrade A. platensis cell wall, improving its nutrients bioavailability for monogastric diets with the cost-effective advantage use of microalgae in animal feed industry. K E Y W O R D SArthrospira platensis, carbohydrate-active enzymes, cell wall, fatty acids, reducing sugars, total proteins

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“…Etkili bir mikroalgal lipit üretimini sağlamak için lipit içeriği ve biyokütle üretim hızı eş zamanlı düşünülmelidir. Yüksek oranda hücresel lipit, çözülebilir polisakkarit ve protein içeren mikroalgler; biyogaz ve biyoyağlara kolaylıkla piroliz edilebilir [33]. Yani sadece yağ değil, proteinler ve suda çözünen karbonhidrat bileşenleri de termokimyasal yöntemlerle kolayca gaz ve yakıtlara dönüştürülebilir [46], [47].…”

Section: Parametrelerunclassified

Sustainable and Eco-Friendly Raw Materials for Biofuels: Microalgae

Eleren¹,

Öner²

2019

Pamukkale J Eng Sci

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Son yıllarda petrol rezervlerinin hızlı tüketilmesi ve buna bağlı olarak meydana gelen sera gazlarının çevre üzerinde olumsuz etkileri neticesinde, endüstriyel ekonomi ve toplum tüketimi için sürdürülebilir ve çevre dostu alternatif enerji kaynağı olan mikroalgler ön plana çıkmıştır. Bu çalışma kapsamında, biyoyakıt üretim proseslerinin seçimini etkileyen mikroalglerin kimyasal kompozisyonu, mikroalg kültürü yetiştirilmesini etkileyen faktörler, kültür yetiştirmede kullanılan sistemler, biyoyakıt üretim prosesleri ve ekonomik analizleri incelenmiştir. Mikroalglerin içeriği, dönüştürme proseslerini etkilemekte ve elde edilen biyoyakıtlar farklılık göstermektedir. Bu inceleme neticesinde, mikroalgler kullanılarak biyodizel, biyoetanol, metan ve mikroalg kalıntılarının yakılması veya gazlaştırılması ile ısı ve elektrik üretiminin söz konusu olabileceği tespit edilmiştir. Mevcut teknolojiler ile biyoyakıt üretimi için alg yetiştirilmesinin tam ölçekli uygulamaları oldukça pahalıdır. Alg üretim maliyetlerini azaltmak için bölgesel enerji santralleri veya endüstriyel baca gazı ve atıksu arıtma tesisleri etkili ve entegre bir şekilde kullanılabilir.Microalgae, a sustainable and environmentally friendly alternative energy source for the industrial economy and community consumption, has come to the forefront in recent years due to the rapid depletion of oil reserves and consequent negative effects of greenhouse gases on the environment. In this study, the chemical composition of microalgae affecting the selection of biofuel production processes, factors affecting microalgae cultivation, cultivated systems, biofuel production processes and its economic analysis are examined. The content of microalgae affects the conversion processes, and the obtained biofuels from microalgae show differences. As a result of this investigation, it has been concluded that the heat and electricity production may be obtained from burning or gasification of microalgae residues. Biodiesel, bioethanol and methane can also be produced using microalgae. The full-scale applications of algae cultivation for biofuel production with existing technologies are quite expensive. To reduce algal production costs, the regional power plants or the industrial flue gas and the wastewater treatment plants should be used in an effectively and integrally.

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An integrative process for obtaining lipids and glucose from Chlorella vulgaris biomass with a single treatment of cell disruption

Cited by 35 publications

References 33 publications

Evaluation of disruption/permeabilization methodologies for Microcystis aeruginosa as alternatives to obtain high yields of microcystin release

Evaluation of disruption/permeabilization methodologies for Microcystis aeruginosa as alternatives to obtain high yields of microcystin release

A two‐enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets

Sustainable and Eco-Friendly Raw Materials for Biofuels: Microalgae

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