An integrative strategy for discovery of functional compound combination from Traditional Chinese Medicine: Danhong Injection as a model

Wang, Zhixiong; Wan, Haofang; Tong, Xiuzhen; Yang, Jiehong; Zhang, Ling; Shao, Chongyu; Ding, Zhongxiang; Wan, Haitong; Li, Chang

doi:10.1016/j.biopha.2021.111451

Cited by 17 publications

(8 citation statements)

References 34 publications

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“…A unique four-step strategy comprising of 'Global profiling', 'Chemical structural classification', 'Intra-group screening', and 'Component-knockout optimization' was adopted to screen and optimize the functional compound combination (FCC) from traditional Chinese medicine (TCM). The holistic neuroprotective effects of the FCC consisting of quercetin indicated antioxidative and anti-inflammatory responses in PC12 cells to save them from oxidative stress, as reported by Wang et al [71]. The group hypothesized that the reported strategy could offer an alternative to discover phytochemicals of biological importance, such as quercetin used in traditional medicines.…”

Section: Quercetin As a Neuroprotective Agentmentioning

confidence: 70%

Quercetin: A Bioactive Compound Imparting Cardiovascular and Neuroprotective Benefits: Scope for Exploring Fresh Produce, Their Wastes, and By-Products

Bhat

2021

Biology

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Quercetin, a bioactive secondary metabolite, holds incredible importance in terms of bioactivities, which has been proved by in vivo and in vitro studies. The treatment of cardiovascular and neurological diseases by quercetin has been extensively investigated over the past decade. Quercetin is present naturally in appreciable amounts in fresh produce (fruits and vegetables). However, today, corresponding to the growing population and global demand for fresh fruits and vegetables, a paradigm shift and focus is laid towards exploring industrial food wastes and/or byproducts as a new resource to obtain bioactive compounds such as quercetin. Based on the available research reports over the last decade, quercetin has been suggested as a reliable therapeutic candidate for either treating or alleviating health issues, mainly those of cardiovascular and neurological diseases. In the present review, we have summarized some of the critical findings and hypotheses of quercetin from the available databases foreseeing its future use as a potential therapeutic agent to treat cardiovascular and neurological diseases. It is anticipated that this review will be a potential reference material for future research activities to be undertaken on quercetin obtained from fresh produce as well as their respective processing wastes/byproducts that rely on the circular concept.

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Section: Quercetin As a Neuroprotective Agentmentioning

confidence: 70%

Quercetin: A Bioactive Compound Imparting Cardiovascular and Neuroprotective Benefits: Scope for Exploring Fresh Produce, Their Wastes, and By-Products

Bhat

2021

Biology

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“…Danhong injection (DHI) is a modern Chinese medical product that is prepared from Salvia miltiorrhiza Bunge [Lamiaceae] (Danshen) and Carthamus tinctorius L. [Asteraceae] (Honghua). DHI has multiple pharmacological properties, including anti-oxidant, anti-apoptotic, anti-inflammatory, anti-coagulation, and pro-angiogenesis, and is widely applied in cardio- and cerebro-vascular diseases ( Feng et al, 2019 ; Wang et al, 2021 ). In our previous study, the protective effect of DHI on DOX-induced cardiotoxicity was preliminarily confirmed in H9c2 cells ( Yi et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Danhong injection attenuates doxorubicin-induced cardiotoxicity in rats via suppression of apoptosis: network pharmacology analysis and experimental validation

Wang

Feng

et al. 2022

Front. Pharmacol.

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Doxorubicin (DOX) is a potent chemotherapeutic agent that is used against various types of human malignancies. However, the associated risk of cardiotoxicity has limited its clinical application. Danhong injection (DHI) is a Chinese medicine with multiple pharmacological activities and is widely used for treating cardiovascular diseases. The aim of the present study was to evaluate the potential protective effect of DHI on DOX-induced cardiotoxicity in vivo and to investigate the possible underlying mechanisms. First, a sensitive and reliable HPLC−ESI-Q-TOF-MS/MS method was developed to comprehensively analyze the chemical compositions of DHI. A total of 56 compounds were identified, including phenolic acids, tanshinones, and flavonoids. Then, a DOX-induced chronic cardiotoxicity rat model was established to assess the therapeutic effect of DHI. As a result, DHI administration prevented the reduction in body weight and heart weight, and improved electrocardiogram performance. Additionally, the elevated levels of serum biochemical indicators were reduced, and the activities of oxidative enzymes were restored in the DOX-DHI group. Network pharmacology analysis further revealed that these effects might be attributed to 14 active compounds (e.g., danshensu, salvianolic acid A, salvianolic acid B, rosmarinic acid, and tanshinone IIA) and 15 potential targets (e.g., CASP3, SOD1, NOS3, TNF, and TOP2A). The apoptosis pathway was highly enriched according to the KEGG analysis. Molecular docking verified the good binding affinities between the active compounds and the corresponding apoptosis targets. Finally, experimental validation demonstrated that DHI treatment significantly increased the Bcl-2 level and suppressed DOX-induced Bax and caspase-3 expression in rat heart tissue. Furthermore, DHI treatment obviously decreased the apoptosis rate of DOX-treated H9c2 cells. These results indicate that DHI attenuated DOX-induced cardiotoxicity via regulating the apoptosis pathway. The present study suggested that DHI is a promising agent for the prevention of DOX-induced cardiotoxicity.

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“…However, few bioinformatics analyses examining the molecular mechanisms, differentially expressed genes (DEGs), and differentially accumulated metabolites (DAMs) of PG grown in different geographic regions have been conducted, and this has impeded the development and utilisation of PG. The molecular mechanisms of medicinal plants involve multiple targets, multiple pathways, and networked processes 27 . Thus, traditional research methods are often insufficient for acquiring a complete understanding of these mechanisms.…”

Section: Introductionmentioning

confidence: 99%

“…The molecular mechanisms of medicinal plants involve multiple targets, multiple pathways, and networked processes. 27 Thus, traditional research methods are often insufficient for acquiring a complete understanding of these mechanisms. Several transcriptomic and metabolomic methods have been used to study medicinal plants, and these studies have provided new insights into the growth, physiology, secondary metabolites, biosynthesis, and key genes of various medicinal plants.…”

mentioning

confidence: 99%

Integrated metabolomic and transcriptomic analysis reveals variation in the metabolites and genes of Platycodon grandiflorus roots from different regions

Chang

Pei

et al. 2022

Phytochemical Analysis

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Introduction Platycodon grandiflorum root (PG), a popular traditional Chinese medicine, contains considerable chemical components with broad pharmacological activities. The complexity and diversity of the chemical components of PG from different origins contribute to its broad biological activities. The quality of southern PG is superior to that of northern PG, but the mechanisms underlying these differences remain unclear. Objectives In order to study variation in the differentially accumulated metabolites (DAMs), differentially expressed genes (DEGs), as well as their interactions and signalling pathways among PG from Anhui and Liaoning. Methods The metabolomes based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) and the transcriptome based on high‐throughput sequencing technology were combined to comprehensively analyse PGn and PGb. Results A total of 6515 DEGs and 83 DAMs from the comparison of PG from Anhui and Liaoning were detected. Integrated analysis of metabolomic and transcriptomic data revealed that 215 DEGs and 57 DAMs were significantly enriched in 48 pathways according to KEGG pathway enrichment analysis, and 15 DEGs and 10 DAMs significantly enriched in the main pathway sesquiterpenoid and triterpenoid and phenylpropanoid biosynthesis might play a key role in complex response or regulatory processes. Conclusion Differences in PG from southern and northern China might thus stem from differences in environmental factors, such as precipitation, light duration, and humidity. The results of our study provide new insight into geographic variation in gene expression and metabolite accumulation and will enhance the utilisation of PG resources.

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An integrative strategy for discovery of functional compound combination from Traditional Chinese Medicine: Danhong Injection as a model

Cited by 17 publications

References 34 publications

Quercetin: A Bioactive Compound Imparting Cardiovascular and Neuroprotective Benefits: Scope for Exploring Fresh Produce, Their Wastes, and By-Products

Quercetin: A Bioactive Compound Imparting Cardiovascular and Neuroprotective Benefits: Scope for Exploring Fresh Produce, Their Wastes, and By-Products

Danhong injection attenuates doxorubicin-induced cardiotoxicity in rats via suppression of apoptosis: network pharmacology analysis and experimental validation

Integrated metabolomic and transcriptomic analysis reveals variation in the metabolites and genes of Platycodon grandiflorus roots from different regions

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