I dedicate this work to my father, Ronaldo César Borges, and to mother, Janise Ruela Pereira Borges. I would like to thank my advisor, Professor Maria Cristina Ferreira de Oliveira, for receiving me as her PhD. candidate, for the patience during the last years, for the instructions when we were conducting this work and for the support during my evolution as a researcher. I am very pleased to be able to attend to meetings, discuss research and share ideas. I also would like to thank Prof. Bernd Hamann for receiving me as a visiting research scholar in UC Davis and for taking your time with me to supervise my research during my time in United States. I wish to acknowledge the finnancial support provided by FAPESP-the State of São Paulo Research Funding Agency, procs. 2012/00269-7 and 2013/26647-0. I also would like to thank the support of my friends from Uberlândia, São Carlos, Davis (Califórnia) and Lavras. There have been so many years in this journey and friends are always a part of that. I'll never forget the good and bad times and I'll take you in my heart for the rest of my life. "However difficult life may seem, there is always something you can do and succeed at. " (Stephen Hawking