An Inter-Subspecies Cloned Buffalo (Bubalus bubalis) Obtained by Transferring of Cryopreserved Embryos via Somatic Cell Nuclear Transfer

Abstract

ContentsThe aim of this study was to explore the feasibility of cryopreservation of inter-subspecies cloned embryos in buffalo. In our experiment, river buffalo ear fibroblast nucleus was fused into swamp buffalo oocyte cytoplasm. The blastocyst formation rate for nuclear transfer of freshly thawed cells was not different from those of growing cells, confluent or serumstarved cells. A total of 122 cloned blastocysts derived from cryopreserved fibroblasts were cryopreserved and thawed, 37 were survived, the cryosurvival rate was 30.3%. The survived blastocysts were transferred into 15 recipient buffalos. Five of the recipients established pregnancy, but four of them aborted on day 53, 59, 145 and 179 of gestation respectively. One crossbred buffalo (Murrah · Swamp buffalo (2n = 49) received three embryos delivered a 40.5 kg female calf by natural delivery on day 320 of gestation. Up to now (13-month old), the cloned calf has been growing well with no abnormity observed. These results demonstrated that cryopreservation of inter-subspecies cloned embryos is feasible to produce buffalo offspring.

Extranuclear Inheritance of Mitochondrial Genome and Epigenetic Reprogrammability of Chromosomal Telomeres in Somatic Cell Cloning of Mammals

“…(3) sheep [21][22][23][24][25][26]; (4) pigs [27][28][29][30][31][32][33][34][35][36][37][38][39][40][41]; (5) equids-domestic horses [42][43][44][45][46] and mules [47]; (6) water buffaloes-Chinese swamp buffaloes [48,49] and Indian river/riverine buffaloes [50][51][52][53]; (7) one-humped or dromedary camels [7,[54][55][56]; (8) two-humped or Bactrian camels [57]; (9) domestic cats [58][59][60][61][62][63]; (10) domestic dogs…”

Epigenetic manipulation to improve mouse SCNT embryonic development

“…Dolly, the first cloned mammal produced by SCNT, was born in 1996 ( Wilmut et al, 1997 ). Since then, about 25 mammalian species have been cloned by SCNT ( Wakayama et al, 1998 ; Lanza et al, 2000 ; McCreath et al, 2000 ; Onishi et al, 2000 ; Loi et al, 2001 ; Chesné et al, 2002 ; Forsberg et al, 2002 ; Keefer et al, 2002 ; Loi et al, 2002 ; Shin et al, 2002 ; Galli et al, 2003 ; Woods et al, 2003 ; Zhou et al, 2003 ; Gómez et al, 2004 ; Lee et al, 2005a ; Lee et al, 2005b ; Lagutina et al, 2005 ; Lan et al, 2006 ; Li et al, 2006 ; Berg et al, 2007 ; Green et al, 2007 ; Kim et al, 2007 ; Shi et al, 2007 ; Gómez et al, 2008 ; Folch et al, 2009 ; Hoshino et al, 2009 ; Wani et al, 2010 ; Yang et al, 2010 ; Hwang et al, 2013 ; Wani et al, 2017 ; Lu et al, 2018 ; Gavin et al, 2020 ), including our successful cloning of non-human primates ( Liu et al, 2018b ; Liu et al, 2019 ). Indeed, SCNT has great potential applications in many fields, such as agro-biotechnology, endangered species conservation, disease model production, and regenerative medicine ( Kim et al, 2007 ; Rogers et al, 2008 ; Gómez et al, 2009 ; Hickey et al, 2011 ; Chung et al, 2014 ; Lu et al, 2014 ; Yamada et al, 2014 ; Chung et al, 2015 ; Lu et al, 2015 ).…”

Buffalo (Bubalus bubali) Late Embryo and Foetus Development: A Morphological Analysis

“…Recent studies have focus on mean fields as: artificial insemination, in vitro fertilization (Meena and Das ), development and reprogramming of buffalo nucleus transfer embryos (Yang et al. ; Luo et al. ).…”

“…Recent studies have focus on mean fields as: artificial insemination, in vitro fertilization (Meena and Das 2006), development and reprogramming of buffalo nucleus transfer embryos (Yang et al 2010;Luo et al 2013). The early gestation stage in buffalo was described by Hafez and Hafez (2004) and Anwar and Ullah (1998) showing embryonic development at 7 days after implantation, and Morini et al (2013Morini et al ( , 2015 described the developmental change in organs of early embryos (10-60 days of pregnancy).…”