An interaction between a C-type lectin receptor and leukocyte cell-derived chemotaxin 2 of ayu, Plecoglossus altivelis

Prokaryotic expression, purification, and refolding of leukocyte cell-derived chemotaxin 2 and its effect on gene expression of head kidney-derived macrophages of a teleost fish, ayu (Plecoglossus altivelis)

“…Recently, studies on the immune response in some fish showed that LECT2 transcripts increased dramatically after bacterial infections, shedding new lights on the function of this gene in fish [8,9]. Most recently, we found for the first time that ayu LECT2 mature peptide (aLECT2m) could interact with the single extracellular C-type lectin domain of a C-type lectin receptor (CLR) [10], which is one of pattern recognition receptors (PRRs) present on fish immune cells [11]. Therefore, it would be interesting to make clear whether aLECT2m could regulate ayu macrophages.…”

Molecular characterization and functional analysis of a novel C-type lectin receptor-like gene from a teleost fish, Plecoglossus altivelis

“…1 385 and 2). PaCD209L is predicted to be a type II transmembrane protein with a [11]. In this study, the PaCD209L transcripts were widely distributed in ayu tissues, 416 with high abundance in the brain and heart (Fig.…”

“…However, 51 some CRDs reportedly bind to carbohydrates in a calcium-independent manner [3]. 52 To date, many fish CLR genes have been sequenced, such as a mannose receptor [11]. 58 Fish CLRs have been reported to be involved in pathogen-associated molecular patter The bacteria-binding activity of rPaCD209L was assayed as previously described [21].…”

“…59ns (PAMPs) recognition, phagocytosis and clearance of bacteria by phagocytic cells 60[5][6][7][8][9][10][11]. Although the functions of CLRs have been extensively studied in mammals, of fish CLRs in regulation of the immune system keeps less known.…”

The establishment of a library screening method based on yeast two-hybrid system and its use to determine the potential interactions of liver proteins in ayu, Plecoglossus altivelis

“…CLR, a monomeric transmembrane receptor in the surface of fish leukocytes [16,17], is possibly involved in the induction of leukocyte intracellular signals.…”