An Internal View of the Spherical Body of <i>Treponema macrodentium</i> as Revealed by Scanning Electron Microscopy

Umemoto, Toshihiko; Namikawa, Isamu; Yoshii, Zensaku; Konishi, Hisanori

doi:10.1111/j.1348-0421.1982.tb00171.x

Cited by 12 publications

(11 citation statements)

References 5 publications

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“…1d). Thin crosssections of spherical bodies examined by TEM revealed they contained loosely associated electron dense material (Figs 1d-f), and consistent with features recorded for other spirochaetes (Umemoto & Namikawa, 1980;Umemoto et al, 1982;Wolf et al, 1993;Brorson & Brorson, 1998), the protoplasmic cylinders were always located immediately beneath the outer membrane (Figs 1d-f). Spherical bodies sized 2-3 mm diameter, each contained six to eight crosssections of the protoplasmic cylinder but the larger bodies (6-12 mm in diameter), showed an increase usually between 10 and 14 cross-sections per body.…”

Section: Internal Ultrastructure Of Spherical Bodiessupporting

confidence: 85%

Spherical body formation in the spirochaeteBrachyspira hyodysenteriae

Wood¹,

Seviour

Siddique

et al. 2006

FEMS Microbiology Letters

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When cultures of Brachyspira hyodysenteriae were grown under a wide range of in vitro conditions, at least 1% of the cells formed spherical bodies different to the normal helical form. This percentage increased considerably in aging cultures or following their incubation in caramelized media. Spherical body formation was initiated from a terminal localized swelling of the outer sheath followed by a retraction of the protoplasmic cylinder into the resulting swollen vesicle. As this occurred, the periplasmic flagella seemed to unwind from the protoplasmic cylinder. Once retracted, the protoplasmic cylinder was found to be wrapped in an organized manner around the inner surface of the membrane of the swollen vesicle. Although most were 2-3 microm in diameter, some much larger spherical bodies (6-12 microm diameter) were occasionally seen, with a corresponding increase in the visible number of peripheral protoplasmic cylinder cross-sections. Spherical bodies from older cultures did not contain protoplasmic cylinders arranged around the periphery, but instead were characterized by the presence of a centrally located, electron-dense body c. 0.5-0.8 mum in diameter. Brachyspira hyodysenteriae spherical bodies differ in both their structural organization and probable method of formation from similar structures described in other spirochaete genera.

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Section: Internal Ultrastructure Of Spherical Bodiessupporting

confidence: 85%

Spherical body formation in the spirochaeteBrachyspira hyodysenteriae

Wood¹,

Seviour

Siddique

et al. 2006

FEMS Microbiology Letters

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“…When the microorganisms from young colonies, for instance, 4-day-old colonies, to be morphologically quite similar to the "spherical bodies"produced abundantly by the oral treponeme strain G7201 in sterility test broth containing a high con centrationof sucrose (15,16).…”

Section: Resultsmentioning

confidence: 99%

“…A human oral treponeme, strain G7201, was isolated from a human oral cavity in our laboratory. These treponemes were subcultured in sterility test broth supplemented with 12.5% horse serum (16).…”

Section: Methodsmentioning

confidence: 99%

Colonial Morphology of Treponemes Observed by Electron Microscopy

Umemoto¹,

Namikawa²,

Yamamoto³

1984

Microbiology and Immunology

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The colonial morphology of three strains of cultivable, nonpathogenic treponemes including a human oral treponeme was examined by light and electron microscopy. Treponema phagedenis strains Kazan and Reiter produced large white colonies on the surface of solid media composed of sterility test broth, 0.9 to 3.1% agar, rifampin, and 12.5% rabbit or horse serum. A human oral treponeme, strain G7201, grew as diffused white zones on 0.9 to 3.1% agar plates. Under the cultural conditions employed agar concentrations slightly affected the time of appearance of colonies of the three strains of treponemes. When the colonies of these three strains were viewed by scanning electron microscopy, differences in their colonial morphology were observed. The 11‐day‐old colonies of human oral strain G7201 were very small, 5 to 15 μm in diameter, and had a slight irregular border. Kazan treponemes developed circular, entire and low convex colonies. Scanning and transmission electron microscopy revealed that the colonies of Reiter treponemes contained spherical forms almost up to 5 μm in diameter, each consisting of an outer membrane and a treponemal main body. They were very similar to the spherical bodies produced by strain G7201 in sucrose‐containing broth.

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“…Both spirochaetal structures were assembled underneath the membrane of the cyst-like structure; the axial fibrils wound around the protoplasmic cylinders (Figure 3e). To our knowledge, only one electron microscopic study has been published on a similar cyst-like structure (spherical body) formed by Treponema macrodentium (10). In the vicinity, but on the outside of the cyst-like structure, mature spirochaetes adhering to the membrane appeared to have been released by intracystic structures (Figure 3f), Until now it was thought that transverse fission is the main mode of spirochaetal reproduction (5,9) (Figure 2).…”

Section: Methodsmentioning

confidence: 99%

Unusual in vitro formation of cyst-like structures associated with human intestinal spirochaetosis

Gebbers

Marder

1989

Eur. J. Clin. Microbiol. Infect. Dis.

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Spirochaetes were isolated from rectal biopsies of three patients and successfully cultured. Enzymatic reactions and electron microscopy revealed spirochaetes resembling Brachyspira aalborgi. Examination of ultrathin sections of centrifugates of cultured spirochaetes yielded unusual cyst-like structures with an outer double membrane containing spirochaetes in different developmental stages. The protoplasmic cylinders seemed to originate from a large electron-dense focus in the cyst-like structures. The axial fibrils occurred as loosely distributed solitary structures in the cyst-like structures. Cysts were not found in the biopsy tissue. The encystment of the spirochaetes could be related to their protection, multiplication, spread and transmission.

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An Internal View of the Spherical Body of Treponema macrodentium as Revealed by Scanning Electron Microscopy

Cited by 12 publications

References 5 publications

Spherical body formation in the spirochaeteBrachyspira hyodysenteriae

Spherical body formation in the spirochaeteBrachyspira hyodysenteriae

Colonial Morphology of Treponemes Observed by Electron Microscopy

Unusual in vitro formation of cyst-like structures associated with human intestinal spirochaetosis

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