An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

Short columns with molecularly imprinted monolithic stationary phases for rapid separation of diastereomers and enantiomers

“…4a, the separation of diastereomers on the prepared disk was similar with that obtained on the long columns and the separation factor of diastereomers on the disk was about 5.75. It turned out that a multiple adsorption/desorption process also occurs in the disk [12,30]. Subsequently, effect of temperature on separation factor on the disk was investigated in the range of 30-70 • C. With increasing temperature, the separation factor increased, which was in agreement with the previous report obtained on the long column [25].…”

“…Large through-pores present in this type of stationary phase allow mobile phases to flow through the sorbent with low flow resistance at high flow rates and convection becomes a dominant mass transport mechanism, which is much more rapid than diffusion in conventional stationary phases [7][8][9]. A variety of applications of rapid separation on this type of stationary phase have been reported [10][11][12]. In 1993, Matsui et al [13] employed the in situ polymerization technique to prepare molecularly imprinted monolithic polymer rods.…”

Application of immobilized enzyme reactor in on-line high performance liquid chromatography: A review

“…Infact when microparticulate supports are employed, the system operates under diffusionlimiting conditions because the substrate reaches the catalytic sites more slowly than the rate of enzymatic product formation [8]. Now with the introduction of monolithic supports it is possible to achieve a total absence of diffusion limitations so that the enzymatic kinetic parameters were flow-unaffected [9,10].…”

Synthesis of a monolithic, micro-immobilised enzyme reactor via click-chemistry

“…This extraordinary behaviour was explained by the enhanced diffusivity of substrate molecules in the monoliths where mass transfer was controlled by convection rather than diffusion. The monolithic minicolumn also contained a homogeneous pore structure with highly interconnected, micron-size, large pores of 1.5 microns in diameter [35][36][37]. Highly interconnected, micron-size, large pores can be also clearly seen in the SEM photo of our monolithic microcolumn.…”