An Introduction to Theory

Bruns, Daniela; Gabriel, Sonja

doi:10.14361/9783839453292-020

Cited by 1 publication

(1 citation statement)

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“…Our results demonstrated that two sets of primers, ITS1F/ITS4 and LROR/LR6, used to analyze the nucleotide regions of rRNA-ITS and 28S rRNA, respectively, accurately identi ed the wild mushroom sample isolates UBU_LS1 and UBU_LP2 as Lentinus squarrosulus and Lentinus polychrous, respectively. As previously reported, the nucleotide ITS region is variable [25] and was not appropriately used alone; thus, using different oligonucleotide primers in PCR for detecting and analyzing different DNA regions could be supplemented in mushrooms and other fungal species.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the prebiotic potential of crude polysaccharides extracted from wild Lentinus polychrous and Lentinus squarrosulus and their application in the development of a novel lyophilized synbiotic

Panya,

Kaewreamruaen,

Saenwang

et al. 2023

Preprint

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Background: Edible mushrooms, including wild mushrooms, are currently being investigated as natural sources to evaluate their prebiotic potential. This study aimed to evaluate the prebiotic potential of crude polysaccharides extracted from wild Lentinus squarrosulus UBU_LS1 and Lentinus polychrous UBU_LP2 and their application as cryoprotectants in the freeze-drying process to formulate a novel synbiotic product. Results: Wild edible mushrooms L. squarrosulus UBU_LS1 and L. polychrous UBU_LP2 contained high amounts of crude polysaccharides (CPS) after hot water extraction. Monosaccharide component analysis showed that CPS_UBU_LS1 and CPS_UBU_LP2 were typical heteropolysaccharides. CPS_UBU_LS1 and CPS_UBU_LP2 showed hydrolysis tolerance to the simulated human gastric acidic pH solution, indicating the possibility of CPS reaching the lower gastrointestinal tract. Antioxidant activity determined using the 1,1-diphenyl-2-picrylhydrazyl assay revealed that the CPS_UBU_LS1 and CPS_UBU_LP2 displayed greater antioxidant activities comparable with that of ascorbic acid. We observed that CPS_UBU_LS1 and CPS_UBU_LP2 have a high potential for stimulating growth in all probiotic strains. Moreover, both CPS compounds may be used as cryoprotectants in freeze drying, since the viability of the selected probiotic L. fermentum 47-7 exhibited cell survival of greater than 70% after 90 days of storage at 4 °C. Conclusion: Based on our results, the wild edible mushrooms Lentinus squarrosulusand Lentinus polychrous may be natural sources of prebiotics for the development of synbiotic products. Further evaluation of these prebiotics, such as the potential for modulation of human gut microbiota in healthy or diseased individuals, is being conducted by our research group.

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Section: Discussionmentioning

confidence: 99%