An Investigation into Bacterial Bloodstream Infections and Antibiotic Resistance Profiles in a Tertiary Hospital for a Ten-Year Period

Mataj, Valbona; Güney, Mustafa; Sığ, Ali Korhan; Güçlü, Aylin Üsküdar; Albay, Ali; Bedir, Orhan; Baysallar, Mehmet

doi:10.7754/clin.lab.2020.191033

Cited by 10 publications

(6 citation statements)

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“…Second, regarding MDR bacteria, the most common mechanism noticed in this study was the production of ESBL by Klebsiella spp., E. coli or Enterobacter (47% of all MDR cases). This observation became standard and was also reported by Mataj et al and Wang et al in Asia [15,16], Acma et al in America [17], and Arana et al in Europe [18].…”

Section: Discussionsupporting

confidence: 72%

Multi-Drug Resistant Bacteria as Aetiological Factors of Infections in a Tertiary Multidisciplinary Hospital in Poland

Poletajew

Pawlik²,

Bonder-Nowicka

et al. 2021

Antibiotics

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Global and local initiatives were recently undertaken to reduce the burden of antibiotic resistance. The aim of the study was to describe the incidence and the aetiology of bacterial infections among hospitalized patients with special attention paid to the multidrug resistant (MDR) bacteria. This retrospective study was based on prospectively collected data from 150,529 consecutive patients hospitalized in a tertiary multidisciplinary hospital in the years 2017–2019. All consecutive microbiological tests from any biological material performed in the analyzed period were included. Microbiological screening tests (n = 10,677) were excluded. The analysis was focused on aetiological factors of bacterial infections, especially the incidence of MDR bacteria and mechanisms of antibiotic resistance. There were 58,789 microbiological tests performed in the analyzed period. The highest testing rate was noticed for intensive care unit (mean of 3.1 tests per one patient), followed by neonatal intensive care unit (2.7), internal medicine (1.9), pediatrics (1.8), and urology (1.2). Among 58,789 tests, 7690 (13.1%) were positive. MDR bacteria were responsible for 1783 infections (23.2%). The most common antibiotic resistance mechanism reported was ESBL production by Klebsiella spp. or Escherichia coli or Enterobacter spp. isolates (47.3% of all MDR cases). ESBL cases were followed by MRSA (14.7%), VRE (14.2%) and MBL producing Klebsiella spp. (5.6%). Among all infections caused by MDR bacteria, 1175 (65.9%) were diagnosed after 72 h of hospitalization (hospital-acquired infections). Apart from AmpC and ESBL producing Escherichia coli, all MDR bacteria were significantly more common in hospital-acquired infection. MDR bacteria are aetiological factors of a significant portion of infections in hospitalized patients with no remarkable change in the incidence in recent years. Production of ESBL is the most common mechanism of antibiotic resistance and should be regarded as one of the most urgent problems in clinical microbiology.

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Section: Discussionsupporting

confidence: 72%

Multi-Drug Resistant Bacteria as Aetiological Factors of Infections in a Tertiary Multidisciplinary Hospital in Poland

Poletajew

Pawlik²,

Bonder-Nowicka

et al. 2021

Antibiotics

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“…On the other hand, BC data seems to be different, since gram negative ones counted with a huge superiority (15). Contrary results were observed in a ten-year BC study from Turkey, since coagulase-negative staphylococci (CoNS) took the largest counts (16). The Turkish National Antimicrobial Resistance Surveillance System (UAMDSS) focuses on antimicrobial resistance (AMR) of E. coli, K. pneumoniae, P. aeruginosa, S. aureus, S. pneumoniae, Enterococcus faecium/faecalis and A. baumannii that were isolated from CSF and BCs.…”

Section: Discussionmentioning

confidence: 93%

Distribution of clinical isolates obtained from sterile body fluids: a four-year retrospective data

Duran

Ati̇k

Özen

et al. 2021

Journal of Health Sciences and Medicine

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Aim: Infections of sterile body fluids (SBFs) require rapid and accurate diagnosis and treatment, since their morbidities and mortalities are high. To achieve this goal, definite epidemiologic data is absolutely required, since empiric and preemptive treatments are mainly based on this. The aim of this study was to evaluate infectious agents isolated from SBFs, susceptibility results and molecular analysis (PCR) data, retrospectively. Material and Method: Clinical samples of SBFs (Cerebrospinal, pleural, peritoneal, pericardial and synovial fluids) obtained from January 2017 to December 2020 in Atatürk City Hospital (tertiary center) were included. Identification of bacterial and fungal agents and antibiotic susceptibility were done by conventional and automated system (BD Phoenix™, Becton Dickinson Co., Sparks, MD, USA). Löwenstein-Jensen media and BACTEC Mycobacteria Growth Indicator Tube 960 (Becton Dickinson Co., Sparks, MD, USA) were used for mycobacterial analysis. Bosphore Viral Meningitis Panel Multiplex PCR Kit (Anatolia Geneworks, İstanbul, Turkey) were applied to detect HSV-1, HSV-2, VZV, Enterovirus and/or Parechovirus. Results: A total of 221 (9.74%) organisms were detected among 2269 samples. Particularly common gram negative bacterial agents covered the top of the list (Escherichia coli, Pseudomonas spp., Klebsiella spp. and Acinetobacter baumannii-Acinetobacter calcoaceticus complex). Staphylococcus aureus was the most frequent gram positive strain, followed by enteroccocci. Most of the A. baumannii isolates were multidrug resistant, Pseudomonas spp. showed over than 20% resistance rate to ceftazidime, cefepime and piperacillin-tazobactam. All enterococci were vancomycin-susceptible, one S. aureus strain was methicillin-resistant. All Mycobacterium tuberculosis complex isolates were found to be susceptible to first-line anti-tuberculosis drugs. Conclusions: Continuous laboratory surveillance even in local phase is important to guide clinicians. Even though our data did not show significant changes, improvements on laboratory capabilities and clinical awareness must be done. Isolation rates might be underestimated due to requirement of improvements in our laboratory, especially about sampling, anaerobe transportation and usage of blood culture vials.

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“…Furthermore, the role of Staphylococcus epidermis in bacteremia is well-known as a bio lm-producing organism with abilities to colonize percutaneous or implanted medical devices and access the bloodstream [26]. This correlates with an international study conducted in both USA and France, where coagulase-negative staphylococci were the main cause of bloodstream infections [27]. Enterobacter and Klebsiella pneumoniae were predominant in sputum cultures.…”

Section: Discussionmentioning

confidence: 69%

Antibiotic sensitivity pattern of bacterial pathogens in two tertiary care hospitals in Damascus, Syria: A cross-sectional study

Abdelwahed¹,

Jassem²,

Kakaje

et al. 2022

Preprint

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Antibiotics resistance is a major concern as it renders antibiotics ineffective, which would have disastrous effects on patient care. The contribution of the Middle Eastern countries in limiting antimicrobial resistance is diminished due to instability and deteriorating healthcare infrastructure. This study aims to report on the antimicrobial resistance pattern in two tertiary care hospitals in Damascus, the capital of Syria in samples of blood, sputum, urine and other. A cross-sectional study based on reports of bacterial cultures was performed between April and November in 2020 in microbiology labs of Damascus Hospital and Al-Razi Hospital. Identification of bacteria was performed using standard microbiological procedures. Susceptibility testing was performed using disk diffusion method. Interpretation of the results was done according to the guidelines of the Clinical and Laboratory Standard Institute. Data were analyzed using the statistical package for social sciences version 26. 412 cultures were positive for pathogens. Most frequently isolated pathogens were Enterobacter, Staphylococcus aureus, and Escherichia coli. Enterobacter was completely sensitive to doripenem and cefoperazone-sulbactam. The resistance rates of Staphylococcus aureus to vancomycin and linezolid were 23.5% and 24.5%, respectively. Escherichia coli was resistant to ciprofloxacin in 72.4% of samples. Klebsiella pneumonia had shown various resistance rates to all used antibiotics. Females had significantly higher frequency of Escherichia coli, Enterobacter, and Klebsiella pneumoniae in urine samples, while males had significantly higher frequency of Pseudomonas aeruginosa and Proteus mirabilis. This study showed the high prevalence of antimicrobial resistance in Damascus Hospital and Al-Razi Hospital, two of major hospitals in Damascus. The decreasing potency of antimicrobials is concerning and may require urgent interventions, such as effective surveillance, and development of national guidelines for prescribing antibiotics.

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An Investigation into Bacterial Bloodstream Infections and Antibiotic Resistance Profiles in a Tertiary Hospital for a Ten-Year Period

Cited by 10 publications

References 0 publications

Multi-Drug Resistant Bacteria as Aetiological Factors of Infections in a Tertiary Multidisciplinary Hospital in Poland

Multi-Drug Resistant Bacteria as Aetiological Factors of Infections in a Tertiary Multidisciplinary Hospital in Poland

Distribution of clinical isolates obtained from sterile body fluids: a four-year retrospective data

Antibiotic sensitivity pattern of bacterial pathogens in two tertiary care hospitals in Damascus, Syria: A cross-sectional study

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