Regular cylinder metallic card clothing has a limited carding efficiency. As a result of the limited dimensions, any measurement between the cylinder and flat area is difficult to make. In this study, an approach is first proposed to simulate the flow field and a fiber finite-element model on the moving surface of the teeth and produce a new design of misaligned-teeth card clothing, with the aim of improving the carding efficiency. A comparison is made between regular and misaligned-teeth card clothing types with respect to flow field simulation and fiber mechanical properties. The results show that the force resulting from the tangential velocity between the cylinder and flat is as great as 1.86 × 10−3 N, sufficient to pull fiber out of tufts, and that the tangential velocity (from 3880 to 2500 mm/s) plays a major role in this area, as opposed to the axial velocity (from 0 to 190 mm/s). Through this comparison, the misalignment design can result in a different tangential velocity distribution from that of traditional card clothing, which helps fibers between two lines of teeth move into neighboring lines of teeth, thereby increasing the likelihood that fibers will be carded. For fiber mechanical analysis, different air forces are loaded on fibers. This comparison shows that for fibers in the channel, the misalignment can help fibers move toward the teeth. Therefore, this misaligned-teeth card clothing is thought to prove more effective in practice.