An investigation of chemical content, enzyme inhibitory propert, antioxidant and antibacterial activity of Aristolochia bodamae Dingler (develiotu) (Aristolochiaceae) root extracts from Samsun, Turkey

Abstract

The present work aimed to evaluate the phytochemical contents of the methanol (ME), ethanol (ET), water (W), acetone (AC), ethyl acetate (EA), and hexane (HE) of Aristolochia bodamae root (ABR) as well as inhibition potential, antioxidative, and antimicrobial activities. The chemical composition analysis and antioxidant activity of ABR extracts were performed by commonly used assays. Antibacterial activities of ABR extracts were tested against three gram‐positive and three gram‐negative bacteria using disk diffusion and microdilution methods. The inhibition potential of the extract was adopted for urease and esterases. The extraction yield (27.10%), total phenolic (47.53 mg gallic acid equivalent/g), and flavonoid (9.74 mg quercetin equivalent/g) content of ABRME were found higher than other extracts. Vanillic acid, caffeic acid, epicatechin, p‐coumaric acid, ferulic acid, and protocatechuic acid were recognized as major components. ABRET, ABRHE, and ABREA extracts had a more effective total antioxidant activity than that of other extracts. The lipid peroxidation inhibition, H2O2, and superoxide scavenging activities of ABRME were found to be 35.94, 35.94, and 3.98 µg/mL, respectively. The ABRET exhibited a reduction force with the A0.5 values of 48.77 µg/mL. The inhibition levels of ABRAC and ABREA to esterases were determined to be more effective than galantamine. ABRME was identified as a more potent urease inhibitor (IC50: 188.42 µg/mL) than thiourea. ABRET demonstrated the antibacterial activity with the highest inhibition zone of 14.4 mm against Staphylococcus aureus. The ABRET found the lowest MIC value against Bacillus cereus (128 µg/mL). In these in vitro studies, it has been found that ABR extracts were a good source of antioxidant, antibacterial, and inhibition potent when evaluated together with phenolic components.

Ecological control of mycotic pathogens in tomato crops - alternatives to synthetic pesticides

“…Which focused on the species A. clematitis L., it was shown that by means of a conventional extraction method and using 60% ethyl alcohol as solvent, the maximum amount of polyphenols recovered from this species was 11.04 mg caffeic acid/g dried plant (Benmehdi et al, 2017). In the case of Aristolochia bodamae Dingler, using the Soxhlet extraction method and the same type of solvent mentioned in the previous study, it was possible to obtain three times the amount of polyphenols, namely 31.11 mg GAE/g, compared to the value recorded for A. clematitis L. (Benmehdi et al, 2017;Ozen et al, 2019). Nile et al (2021) observed a variation in the total content of polyphenols depending on the parts of the C. majus L. plant, which ranged from 7.76 to 23.67 mg/g.…”

Valorization of Polyphenols from Stryphnodendron adstringens Bark for Use as a Sustainable Inhibitor of Nitrogen Volatilization in Soil

“…The results can be seen in Table and the chromatogram in Figure S1. These compounds have already had their antioxidant activities studied, − which proves that these polyphenolic compounds have high potential as enzymatic inhibitors. Barbatimão is a promising species because it has a high concentration of polyphenols in the bark.…”

Biological Activities of Organic Extracts of the Genus Aristolochia: A Review from 2005 to 2021

“…A variety of traditional uses for species of the genus Aristolochia were found in the literature. Of the traditional uses cited, the most common uses are anticancer (33 articles) , antibacterial (31 articles) [23,25,29,30,38,39,45,49,, antioxidants (18 articles) [22,25,29,33,34,46,55,60,61,70,73,74,[77][78][79][80][81][82], snake anti-venom (13 articles) [21,22,39,40,76,[83][84][85][86][87][88][89][90], anti-inflammatory (11 articles) [22,40,46,47,74,86,[91][92][93][94]…”