An investigation of some organic chemicals as cathodoluminescent dyes using the scanning electron microscope

Mets, M. De; Lagasse, A.

doi:10.1111/j.1365-2818.1971.tb03698.x

Cited by 33 publications

(15 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This characteristic of CL suggests a possibility for CL analysis as a new analytical device in biology. However, it has been difficult to utilize CL in biology because CL from biological specimens is generally very weak and easily fades under a high electron beam current (De Mets and Lagasse, 1971). Therefore, a new system which can effectively collect CL signal and minimize the beam effect has been required.…”

Section: Discussionmentioning

confidence: 99%

“…Although CL is essentially a similar phenomenon to conventional fluorescence elicited under ultraviolet light, it has some intrinsic advantages over the latter (Bröcker and Pfefferkorn, 1979;Pfefferkorn et al, 1980): (1) CL can be amplified electronically and measured quantitatively; (2) the depth of focus and the resolution in the CL mode is much higher than in the conventional fluorescence microscope; and (3) some substances exhibit luminescence only under electron irradiation. Although many attempts (Basu, 1983;Bond et al, 1974;Boyde and Reid, 1983;Bröcker and Pfefferkorn, 1979;Cavellier and Berry, 1985;Cavellier et al, 1978;De Mets, 1974;De Mets and Lagasse, 1971;Herbst and Hoder, 1978;Hörl, 1972Hörl, , 1978Hurter et al, 1981;Pearce and Hays, 1966;Schmidt et al, 1975;Soni et al, 1975) were made to utilize CL in biology, its applications have been limited because CL from organic chemicals is a very weak signal and easily fades under high beam current (De Mets and Lagasse, 1971).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Analytical color fluorescence electron microscopy of adrenal cortex

Nakano

Koike

Ogawa

1997

Microsc. Res. Tech.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Analytical color fluorescence electron microscopy of adrenal cortex

Nakano

Koike

Ogawa

1997

Microsc. Res. Tech.

View full text Add to dashboard Cite

“…As for the quickly changeable CL emission of adrenal cortex, we could not identify its origin. Though the relationship between molecular structure and CL luminescence yield has not been established yet (12,13), our data that substances having a double bond in B ring have a capacity of emitting CL is thought to offer one example of this relationship. In order to identify the origin of the LD of zona fasciculata cells more sophisticated devices are required.…”

Section: Color CL Imagesmentioning

confidence: 97%

Color cathodoluminescence images and cathodoluminescence spectra analysis of biological materials.

Itoh

Fujimoto

Koike

et al. 1986

Acta Histochem. Cytochem

View full text Add to dashboard Cite

In an attempt to detect cathodoluminescence (CL), an Akashi scanning electron microscope (SEM) ISI-DS130 was remodeled into a cathodoluminescence electron microscope (CEM or fluorescence electron microscope FEM). A newly shaped mirror, combining three half ellipsoidal mirrors, was located over the specimen holder of the top stage for color CL use. The bottom stage was modified with a half ellipsoidal mirror, photomultiplier tube (PMT) and monochrometer, so that not only CL images, but also CL spectra could be obtained.An effective specimen-preparation method, known as the simple cryo-SEM method (24), was devised for CL observation.With these instruments and methods, color CL images and CL spectra of the biological substances were analyzed.Ito cells of vitamin A (vit A)-loaded rat liver and zona fasciculata cells of rat adrenal cortex emitted intense CL from their lipid droplets (LD). In order to identify the origin of this CL emission, analysis of the color CL images and the CL spectra were available.Cathodoluminescence (CL) is a light emission, in the visible, near ultraviolet and infrared regions under the bombardment of an electron beam (31). CL phenomenon is similar to fluorescence or phosphorescence excited by ultraviolet light, but it has some intrinsic advantages over the fluorescence as described by Brocker et al. (6,31) : 1) CL can be amplified and measured quantitatively, 2) The depth of focus and the resolution in the CL mode are higher than in the fluorescence microscope. 3) Some substances exhibit luminescence only under electron irradiation. Furthermore, CL of organic chemicals stems from loosely bound it electrons; it shows the information of the chemical bond. Just as secondary electrons (SE), backscattered electrons, Auger electrons and X-rays are often used for observing or analysing substances under scanning electron microscope (SEM), so CL should become an effective analytical tool.Although CL studies as an electron probe can be traced to 1952, the CL applications to biological and medical fields have not made remarkable advances.

show abstract

“…Manger & Bessis (1970) have found an increased cathodoluminescence of blood cells, y-globulin, albumin and dried plasma after treatment with formaldehyde vapour. Some fluorescence microscopy stains and products with scintillation properties have been examined in the SEM and their cathodoluminescence yield was determined (De Mets & Lagasse, 1971). Similar experiments have been made on herbicides (Falk, 1972).…”

Section: Introductionmentioning

confidence: 97%