An investigation of the incidence of intersex fish in Seine-Maritime and Sussex region

Minier, Christophe; Caltot, G.; Leboulanger, F.; Hill, Elisabeth M.

doi:10.1051/analusis:2000280801

Cited by 57 publications

(46 citation statements)

References 17 publications

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“…Rutilus rutilus, Xiphias gladius, Acipenser gueldenstaedtii) and invertebrate species, as Mya arenaria, marine and freshwater mussels (e.g. Mytilus galloprovincialis, Tapes philippinarium, Elliptio complanata) (Minier et al 2000;Jobling et al 2002;De Metrio et al 2003;Gagné and Blaise 2003;Canesi et al 2004;Langston et al 2005;Sumpter 2005;Bjerregaard et al 2006;Jackson et al 2006;Porte et al 2006;Almeida et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Incidence of intersex in male clams Scrobicularia plana in the Guadiana Estuary (Portugal)

2009

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Endocrine disrupting chemicals (EDCs) known to be present in the marine environment, have great potential to influence reproductive status in aquatic organisms. A widespread incidence of intersex linked with EDCs exposure has been reported in molluscs and fish species. The species Scrobicularia plana is inherently gonochoristic; nonetheless, the feminisation of male clams was described in estuaries from the United Kingdom. Thus, this study reports the presence of oocytes in the testis of male clams of S. plana collected in the Guadiana River (Portugal). Samples were collected between June and September (2007), and gonad tissue analysed by light microscopy. Intersex with several degrees of intensity was detected during the first three months of sampling, in which the percentage of male clams affected varied between 5.9, 58.3 and 71.4%. Little is known about the causes of intersex in S. plana, being the urban, industrial and agricultural discharges possible major sources of induction. In the Guadiana River, the presence of several EDCs may be directly linked to the incidence of the intersex condition detected in Scrobicularia plana.

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Section: Introductionmentioning

confidence: 99%

Incidence of intersex in male clams Scrobicularia plana in the Guadiana Estuary (Portugal)

2009

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“…In single cases alkylphenols compounds have also been suggested as possible causative agents (Sheahan et al 2002). Induction of vitellogenin (vtg), a biomarker of estrogenic exposure, has been detected in various species of male fish collected downstream of sewage treatment works (STWs) (Jobling et al 1998;Flammarion et al 2000;van Aerle et al 2001;Gercken and Sordyl 2002;Hecker et al 2002;Folmar et al 2001;Harshbarger et al 2000;Minier et al 2000) and in caged fish placed near STW outlets (Harries et al 1997(Harries et al , 2000Larsson et al 1999). After high prevalences of intersex were first observed in roach (Rutilus rutilus) from UK rivers (Jobling et al 1998), other populations of fish with intersex males have been observed downstream of STWs (van Aerle et al 2001;Aravindakshan et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Reproductive disorders among wild populations of fish also include abnormal hormone levels (Folmar et al 1996(Folmar et al , 2002Jobling et al 2002a;Lavado et al 2004), asynchrony in the development of germ cells in males and females as well as reduced spawning and fertilising capacity of male fish (Aravindakshan et al 2004;Jobling et al 2002a, b). Observed feminisation in wild populations of fish does now include the freshwater species roach (Jobling et al 1998: Minier et al 2000, gudgeon (Gobio gobio) (van Aerle et al 2001), carp (Cyprinus carpio) (Folmar 1996;Solé et al 2002a, b), bream (Abramis brama L.) (Hecker et al 2002;Vethaak et al 2002), chub (Leucicus cephalus) (Flammarion et al 2000), shovelnose sturgeon (Scaphirhychus platyorynchus) (Harshbarger et al 2000), walleye (Stizostedion vitreum) (Folmar et al 2001), three spined stickleback (Gasterosteus aculeatus) and perch (Perca fluviatilis) (Gercken and Sordyl 2002) plus the marine species flounder (Platichthys flesus) (Lye et al 1997(Lye et al , 1998Allen et al 1999;Stentiford et al 2003), eelpout (Zoarces viviparus) (Mathiessen et al 2002;Stentiford et al 2003) and two species of sand gobies (Pomatoschistus minutes and P. lozanoi) (Mathiessen et al 2002). Many of the freshwater species which have been examined for signs of feminisation belong to the carp family.…”

Section: Introductionmentioning

confidence: 99%

Gonad histology and vitellogenin concentrations in brown trout (Salmo trutta) from Danish streams impacted by sewage effluent

et al. 2006

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Brown trout (Salmo trutta) collected from a number of Danish streams impacted by sewage effluent were examined for alterations to gonadal development and induction of vitellogenin synthesis. Among fish collected in June/July 2000/2001 and November 2002 higher levels of plasma vitellogenin were found in males from six streams impacted by sewage effluent compared to males from their respective reference sites. A direct non-competitive ELISA was developed for brown trout vitellogenin in order to perform the vitellogenin measurements. Intersex in females with no apparent relation to sewage effluent exposure was observed at all sites. In one stream, male brown trout with a very high level of vitellogenin were concomitantly found to have a high degree of vacuolation of the testes and a presence of only the early spermatogenic stage, spermatogonia. The cause of these alterations to the testis structure is unclear. However, as a high level of plasma vitellogenin in these males indicated estrogenic exposure, the vacuolation might also be a result of endocrine disruption causing delayed or disrupted spermatogenesis.

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“…However, the condition has now been detected in several wild freshwater and migratory species, including roach Rutilus rutilus (Jafri & Ensor 1979, Purdom et al 1994, Jobling et al 1998, gudgeon Gobio gobio (van Aerle et al 2001), barbel Barbus plebejus (Vigano et al 2001), chub Leuciscus cephalus (Minier et al 2000), bream Abramis brama (Slooff & Kloowijk-Vandijk 1982), white perch Morone americana (Kavanagh et al 2002), stickleback Gasterosteus aculeatus (authors' pers. obs.…”

Section: Introductionmentioning

confidence: 99%

First reported cases of intersex (ovotestis) in the flatfish species dab Limanda limanda: Dogger Bank, North Sea

Stentiford¹,

Feist²

2005

Mar. Ecol. Prog. Ser.

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This paper provides the first report of the intersex (ovotestis) condition in the flatfish species dab Limanda limanda. Specimens presenting the condition were collected from the North Dogger Bank in the North Sea during the United Kingdom National Marine Monitoring Programme in 2003. Two of the individuals collected (14.3% of the males sampled) presented externally as males but upon histological examination were seen to contain both male and female gonadal tissues. Previtellogenic oocytes were suspended within a matrix of spermatogonia and spermatocytes. One of the specimens also presented dilated testicular follicles filled with masses of mature spermatozoa. The significance of this finding in the United Kingdom's sentinel offshore monitoring species is discussed with relation to potential offshore effects of exposure to endocrine disrupting chemicals (EDCs). KEY WORDS: Flatfish · Endocrine disruption · Limanda limanda · Ovotestis · Ovary · Testis · Reproduction Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 301: [307][308][309][310] 2005 EDCs extend beyond inland river systems to coastal waters. With the exception of the reports of plasma vitellogenin and ovotestis in male Mediterranean swordfish Xiphias gladius (Fossi et al. 2001 and De Metrio et al. 2003, respectively), little evidence has so far been presented on endocrine disruption in the offshore environment.This study provides the first report of intersex in the United Kingdom National Marine Monitoring Programme's (NMMP) offshore sentinel flatfish species dab Limanda limanda. Two fish presenting the condition were present in a sample of 50 dab collected from the Dogger Bank, an NMMP sampling station in the central North Sea, during 2003. This discovery marks the first histologically confirmed case of intersex in a marine fish species collected from an offshore site. MATERIALS AND METHODSDab Limanda limanda were captured using 30 min tows of a standard Granton trawl on the Northern Dogger Bank, North Sea (55°03' 53'' N, 02°04' 26'' E), as part of the NMMP, in July 2003. The sex, size (total length) and presence of external signs of disease were recorded for 50 fish using methodology specified by the International Council for the Exploration of the Sea (ICES) (Bucke et al. 1996). Following external disease assessment, fish were sacrificed by a blow to the head, followed immediately by severing of the spinal chord. Samples of liver, kidney, spleen and gonad were fixed in neutral buffered formalin for 24 h followed by transfer to industrial methylated spirit. Fixed samples were processed to wax in a vacuum infiltration processor using standard protocols. Sections were cut at 3 to 5 µm on a rotary microtome and resulting tissue sections were mounted onto glass slides before staining with haematoxylin and eosin (H & E). Stained sections were analysed by light microscopy (Eclipse E800, Nikon) and digital images of histological features were obtained using the Lucia™ Screen Measurement System (Nik...

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An investigation of the incidence of intersex fish in Seine-Maritime and Sussex region

Cited by 57 publications

References 17 publications

Incidence of intersex in male clams Scrobicularia plana in the Guadiana Estuary (Portugal)

Incidence of intersex in male clams Scrobicularia plana in the Guadiana Estuary (Portugal)

Gonad histology and vitellogenin concentrations in brown trout (Salmo trutta) from Danish streams impacted by sewage effluent

First reported cases of intersex (ovotestis) in the flatfish species dab Limanda limanda: Dogger Bank, North Sea

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