An iodothyronine deiodinase from Chlamys farreri and its induced mRNA expression after LPS stimulation

Wu, Tiantian; Shi, Xiaowei; Zhou, Zhi; Wang, Lingling; Wang, Mengqiang; Wang, Leilei; Huang, Mengmeng; Yang, Chuanyan; Song, Linsheng

doi:10.1016/j.fsi.2012.05.011

Cited by 19 publications

(9 citation statements)

References 29 publications

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“…Furthermore, these acidic metabolites are the endogenous ligands of B. floridae TH receptors and they control metamorphosis in this species, supporting the notion that even in invertebrate chordates, this distinct, inactivating deiodinative pathway is physiologically relevant (Paris et al 2008(Paris et al , 2010. Interestingly, these invertebrate chordate deiodinase homologs could have an even more ancient origin, and there is evidence that invertebrates like the scallop (Chlamys farreri; Wu et al 2012) and the echinoderm (Strongylocentrotus purpuratus; Heyland et al 2006) may express putative deiodinases. Thus, deiodinase functional diversity in extant vertebrates seems to stem from a common ancestral molecular scaffold that could have already been present in the ancient metazoa.…”

Section: Phylogeny Of Deiodinasessupporting

confidence: 53%

Iodothyronine deiodinases: a functional and evolutionary perspective

Orozco¹,

Valverde-R²,

Olverá³

et al. 2012

Journal of Endocrinology

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From an evolutionary perspective, deiodinases may be considered pivotal players in the emergence and functional diversification of both thyroidal systems (TS) and their iodinated messengers. To better understand the evolutionary pathway and the concomitant functional diversification of vertebrate deiodinases, in the present review we summarized the highlights of the available information regarding this ubiquitous enzymatic component that represents the final, common physiological link of TS. The information reviewed here suggests that deiodination of tyrosine metabolites is an ancient feature of all chordates studied to date and consequently, that it precedes the integration of the TS that characterize vertebrates. Phylogenetic analysis presented here points to D1 as the oldest vertebrate deiodinase and to D2 as the most recent deiodinase gene, a hypothesis that agrees with the notion that D2 is the most specialized and finely regulated member of the family and plays a key role in vertebrate neurogenesis. Thus, deiodinases seem to be major participants in the evolution and functional expansion of the complex regulatory network of TS found in vertebrates.

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Section: Phylogeny Of Deiodinasessupporting

confidence: 53%

Iodothyronine deiodinases: a functional and evolutionary perspective

Orozco¹,

Valverde-R²,

Olverá³

et al. 2012

Journal of Endocrinology

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“…In vertebrates, this kind of enzyme plays a key role in the peripheral regulation of THs actions catalyzing the conversion of T 4 to the active form T 3 (Alves et al, 2017). Although the thyroid system appeared at the beginning of vertebrate evolution, some studies reported thyroxine deiodinases in invertebrates and social amoebas (Heyland et al, 2006;Lobanov et al, 2007;Wu et al, 2012;Singh et al, 2014). Our study is the first report identifying a thyroxine deiodinase in a microalga species with capacity to synthetize TH-like compounds (Heyland and Moroz, 2005) representing one of the most ancestral deiodinase that later propagated in eukaryote lineages.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta)

et al. 2018

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Iodine metabolism is essential for the antioxidant defense of marine algae and in the biogeochemical cycle of iodine. Moreover, some microalgae can synthetize thyroid hormone-like compounds that are essential to sustain food webs. However, knowledge regarding iodine-related molecular processes in microalgae is still scarce. In this study, a de novo transcriptome of Tisochrysis lutea cultured under high iodide concentrations (5 mM) was assembled using both long and short reads. A database termed IsochrysisDB was established to host all genomic information. Gene expression analyses during microalgal growth showed that most of the antioxidant-(aryl, ccp, perox, sod1, sod2, sod3, apx3, ahp1) and iodide-specific deiodinase (dio) genes increased their mRNA abundance progressively until the stationary phase to cope with oxidative stress. Moreover, the increase of dio mRNA abundance in aging cultures indicated that this enzyme was also involved in senescence. Cell treatments with iodide modified the expression of perox whereas treatments with iodate changed the transcript levels of gpx1 and ccp. To test the dependence of perox on iodide, microalgae cells were treated with hydrogen peroxide (H 2 O 2 ) either in presence or absence of iodide observing that several genes related to reactive oxygen species (ROS) deactivation (perox, gpx1, apx2, apx3, ahp1, ahp2, sod1, sod3, and aryl) were transcriptionally activated although with some temporal differences. However, only the expression of perox was dependent on iodide levels indicating this enzyme, acquired by horizontal gene transfer (HGT), could act as a haloperoxidase. All these data indicate that T. lutea activates coordinately the expression of antioxidant genes to cope with oxidative stress. The identification of a phase-regulated deiodinase and a novel haloperoxidase provide new clues about the origin and evolution of thyroid signaling and the antioxidant role of iodine in the marine environment.

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“…To date, THs have been detected in other mollusks using ELISA and thin-layer chromatography (TLC) in the scallop C . farreri [ 14 ] and in the sea hare Aplysia californica [ 12 ], respectively. However, antibodies may cross-link with similar substrates when using the ELISA method and TLC has the disadvantage of low sensitivity and resolution ratio.…”

Section: Discussionmentioning

confidence: 99%

“…gigantea ) [ 13 ]. A deiodinase homolog was cloned and THs were detected in the hemolymph of the scallop Chlamys farreri by enzyme-linked immunosorbent assay (ELISA) [ 14 ]. In the Platyhelminthes, three TR homologs were cloned in Schistosoma mansoni and S .…”

Section: Introductionmentioning

confidence: 99%

Identification of Thyroid Hormones and Functional Characterization of Thyroid Hormone Receptor in the Pacific Oyster Crassostrea gigas Provide Insight into Evolution of the Thyroid Hormone System

Huang

et al. 2015

PLoS ONE

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Thyroid hormones (THs) play important roles in development, metamorphosis, and metabolism in vertebrates. During the past century, TH functions were regarded as a synapomorphy of vertebrates. More recently, accumulating evidence has gradually convinced us that TH functions also occur in invertebrate chordates. To date, however, TH-related studies in non-chordate invertebrates have been limited. In this study, THs were qualitatively detected by two reliable methods (HPLC and LC/MS) in a well-studied molluscan species, the Pacific oyster Crassostrea gigas. Quantitative measurement of THs during the development of C. gigas showed high TH contents during embryogenesis and that oyster embryos may synthesize THs endogenously. As a first step in elucidating the TH signaling cascade, an ortholog of vertebrate TH receptor (TR), the most critical gene mediating TH effects, was cloned in C. gigas. The sequence of CgTR has conserved DNA-binding and ligand-binding domains that normally characterize these receptors. Experimental results demonstrated that CgTR can repress gene expression through binding to promoters of target genes and can interact with oyster retinoid X receptor. Moreover, CgTR mRNA expression was activated by T4 and the transcriptional activity of CgTR promoter was repressed by unliganded CgTR protein. An atypical thyroid hormone response element (CgDR5) was found in the promoter of CgTR, which was verified by electrophoretic mobility shift assay (EMSA). These results indicated that some of the CgTR function is conserved. However, the EMSA assay showed that DNA binding specificity of CgTR was different from that of the vertebrate TR and experiments with two dual-luciferase reporter systems indicated that l-thyroxine, 3,3′,5-triiodothyronine, and triiodothyroacetic acid failed to activate the transcriptional activity of CgTR. This is the first study to functionally characterize TR in mollusks. The presence of THs and the functions of CgTR in mollusks contribute to better understanding of the evolution of the TH system.

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An iodothyronine deiodinase from Chlamys farreri and its induced mRNA expression after LPS stimulation

Cited by 19 publications

References 29 publications

Iodothyronine deiodinases: a functional and evolutionary perspective

Iodothyronine deiodinases: a functional and evolutionary perspective

Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta)

Identification of Thyroid Hormones and Functional Characterization of Thyroid Hormone Receptor in the Pacific Oyster Crassostrea gigas Provide Insight into Evolution of the Thyroid Hormone System

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