An Isolated Perfused Rat Kidney Model for the Evaluation of the Effect of Glucose on Renal Tubular Epithelial Morphology

Zhou, Chong; Yool, Andrea J.; Byard, Roger W.

doi:10.1111/1556-4029.13229

Cited by 3 publications

(7 citation statements)

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“…This consisted of ten Sprague Dawley rats, with body weights ranging from 206 to 420 g (mean 284.8 g) which were perfused for 1 and 2 h with Krebs‐Henseleit buffer in the absence of added triglycerides or ketones. Nonspecific tubular epithelial vacuolizations consistent with osmotic nephrosis were observed in all cases, with the unstained area per high power field ranging from 3.14 to 10.59% (mean 5.73%) in the kidneys perfused for 1 h, and 12.33–33.75% (mean 22.15%) in the kidneys perfused for 2 h. Separation of tubules from the basement membrane was focally observed in some cases, but no discrete subnuclear vacuolizations were present .…”

Section: Resultsmentioning

confidence: 77%

“…The control group was taken from a previous study using the same perfusion system with the same concentration of constituents making up the Krebs‐Henseleit buffer . This consisted of ten Sprague Dawley rats, with body weights ranging from 206 to 420 g (mean 284.8 g) which were perfused for 1 and 2 h with Krebs‐Henseleit buffer in the absence of added triglycerides or ketones.…”

Section: Resultsmentioning

confidence: 99%

“…This model has previously been validated for the investigation of drug effects on glomerular function , renal vasculature , cellular metabolism and gluconeogenesis . A recent study has also shown that it is a viable model for the investigation of renal tubular epithelial histology, showing the induction of osmotic nephrosis subsequent to hyperglycemic perfusate with a statistically significant increase in vacuolization in the hyperglycemia treatment as compared with control perfused kidneys .…”

Section: Discussionmentioning

confidence: 99%

“…Adult Sprague Dawley rats (>200 g each) were euthanized using carbon dioxide (CO 2 ) inhalation. The kidneys were subsequently cannulated through the renal artery via the previously described dissection techniques and placed into a thermostatically controlled cabinet at 34–37.5°C. The cannulated kidney was connected to the recirculating system containing 180 mL of perfusate pumped using a peristaltic pump at approximately 32–38 mL/min with continuous adjustments as necessary to maintain renal artery perfusion pressure at 110 ± 20 mmHg.…”

Section: Methodsmentioning

confidence: 99%

“…However, all studies to date have involved postmortem tissues, which have made the determination of a direct association between basal vacuolization and hyperlipidemia in isolation challenging. The use of a perfused kidney model has allowed separation of possible causal factors, with minimization of other confounding metabolic abnormalities that are often present in these conditions in vivo .…”

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confidence: 99%

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The Etiology of Basal Vacuolizations in Renal Tubular Epithelial Cells Evaluated in an Isolated Perfused Kidney Model

Zhou

Yool

Byard

2016

Journal of Forensic Sciences

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To determine whether basal lipid vacuolization characteristic of ketoacidosis could be induced with short-term hypertriglyceridemia, adult Sprague Dawley rat kidneys were perfused in an isolated perfused kidney model with, and without, 11.3 mM (10 g/L) of triglycerides in Krebs-Henseleit buffer, for 1 and 2 h (n = 5/group). Additional treatments included perfusion with triglycerides with 20 mM of β-hydroxybutyrate and 2 mM of acetoacetate (n = 5) and perfusion with triglycerides with 70 mM of glucose (n = 1). Basal vacuolization was produced in all groups, but differed in morphology to that reported in postmortem studies. There was no further increase in vacuolization after 2 h of perfusion compared to 1 h (p = 0.24), and the addition of ketones did not alter the morphology or extent of vacuolization. This study using an ex vivo model has confirmed that isolated hypertriglyceridemia is sufficient to cause basal lipid vacuolization in renal tubular epithelial cells, but with different morphology to vacuoles observed in lethal ketoacidosis at autopsy.

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Section: Resultsmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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The Etiology of Basal Vacuolizations in Renal Tubular Epithelial Cells Evaluated in an Isolated Perfused Kidney Model

Zhou

Yool

Byard

2016

Journal of Forensic Sciences

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The exacerbated hypokalemia in membranous glomerulonephritis due to proximal tubular injury: a neglect issue from a case report and literature review

Chang¹,

Hou

2023

BMC Nephrol

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Background Membranous glomerulonephritis is the most common primary etiology for the nephrotic syndrome in adults. Beyond the clinical hallmark of nephrotic syndrome such as fluid overloading, dyslipidemia and hypoalbuminemia, the dysregulated homeostasis of potassium and its possible mechanism is seldomly discussed, and its association with the clinical course of membranous GN is lacking. Case presentation A 65 year-old female attended to our emergent department for progressive lower leg edema after taking 15-h of flight. Hypoalbuminemia and hyperlipidemia were both noted, and 24-h urinary total protein was up to 17,950 mg/day. Elevated creatin-phospho-kinase developed at the initial presentation with hypokalemia due to excressive renal excretion. Glycosuria without elevated glycated Hemoglobin occurred. The pathology of kidney biopsy revealed subepithelial immunocomplex deposits with spike formation in the electron microscopy and the positive anti-Phosphlipase A2 receptor antibodies(PLA-2R) with hallmark of membranous glomerulonephritis. In the light microscopy, the vacuolization of proximal tubules was noted, which contributed to the potassium wasting. After 1 year following up duration, the patient’s proteinuria persisted after maintenance treatment with calcineurin inhibitor. Conclusion Hypokalemia is a neglected issue in the membranous glomerulonephritis. Unlike the previous literature, our patient had the vacuolization of proximal tubule at the initial presentation with hypokalemia, which might contribute the potassium wasting. The proximal tubular damage with hypokalemia might be a predictive factor for membranous glomerulonephritis.

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Changes of Renal Morphology and Function in Rats with Muscle Injury after Induced Skeletal Exercise

2020

IJST

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Chronic kidney disease is a progressive disease characterized by chronic renal failure, which can lead to end-stage renal disease over time. Lipid metabolism disorder is a common disease in patients with chronic kidney disease. Lipid can directly or indirectly enhance the progress of renal damage through direct renal cytotoxicity or promoting renal arteriosclerosis. Therefore, it is of great significance to explore the research progress of renal morphology and function in rats with muscle injury induced by skeletal exercise. The purpose of this study is to explore the degree of renal injury and the changes of renal morphology and function after bone exercise, and to find out the relationship between them. In this study, four groups of experimental subjects were set up, which were blank control group, 12-hour exercise group, 24-hour exercise group and 48-hour exercise group. The results showed that the serum uric acid value of the rats after 48 hours of exercise was the highest, reaching 348.1 umol / L, while the serum creatinine index of the blank control group was the highest, which was 112.4 umol / L, which decreased with the increase of exercise time. The highest value of serum urea nitrogen in the blank control group was 5.53 mmol / L. The weight of rats decreased with the increase of exercise time, and the weight loss of rats after 48 hours of exercise was 6.1g. In the quantitative measurement of urinary protein, we can draw such a conclusion that the longer the exercise time, the higher the content of urinary protein, and with the passage of time, the content of urinary protein will return to the normal range, and the measurement results of red blood cell number also increase with the increase of exercise time. Changes of Renal Morphology and Motor FunctionInternational Journal of Sports Technology 34 Acute Kidney InjuryAcutekidneyinjury (Ard) is a syndrome that causes rapid decline in renal function due to various clinical manifestations. It may include multiple organs and systems. Incidence rate is 4%-20% in hospital patients, while in ICU, the morbidity rate in ICU is 30%, and the mortality rate is over 50%. This phenomenon caused by acute kidney injury has become a global medical and social problem. Investigation shows that about 30% of complex acute kidney damage has potential kidney disease, 25.6% secondary to baseline, 34.3% after major surgery, 47.5% with complications after septic shock, 26.9% with cardiac shock and 19% with drug-induced renal damage [4]. Compared with acute renal failure, the complex acute renal damage highlights the importance of early diagnosis and timely treatment of the syndrome. In recent years, it has been widely used and recognized by the medical community.Acute renal damage includes prenatal nitrite, idiopathic acute renal failure and acute tubular necrosis. Aki was described as early as ancient Greece, but there was no index of modern medicine at that time, and the diagnosis was only based on "reduced urine volume". With the development of pathology and biochemistry, mode...

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An Isolated Perfused Rat Kidney Model for the Evaluation of the Effect of Glucose on Renal Tubular Epithelial Morphology

Cited by 3 publications

References 18 publications

The Etiology of Basal Vacuolizations in Renal Tubular Epithelial Cells Evaluated in an Isolated Perfused Kidney Model

The Etiology of Basal Vacuolizations in Renal Tubular Epithelial Cells Evaluated in an Isolated Perfused Kidney Model

The exacerbated hypokalemia in membranous glomerulonephritis due to proximal tubular injury: a neglect issue from a case report and literature review

Changes of Renal Morphology and Function in Rats with Muscle Injury after Induced Skeletal Exercise

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