An isologous antigen-antibody reaction with human neutrophiles, related to neonatal neutropenia.

Lalezari, Parviz; Bernard, G

doi:10.1172/jci105480

Cited by 86 publications

(49 citation statements)

References 16 publications

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“…Family segregation studies document that HGA-3 is not apparently associated with HLA nor with the NA-NB or 5a-5b an-tigens. Absorption studies indicated that the HGA-3 antigens were present only on mature granulocytes, making them comparable in that regard to the neutrophil-specific NA-NB antigens (6,7).…”

Section: Resultsmentioning

confidence: 99%

New Granulocyte Antigens Demonstrated by Microgranulocytotoxicity Assay

Thompson¹,

Overlin²,

Herbick³

et al. 1980

J. Clin. Invest.

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A B S T R A C T Although granulocyte transfusionsand e. Absorptions indicated that these antigens were present on mature granulocytes but absent from platelets, lymphocytes, monocytes, and myeloid precursors. A single antigen of another separate locus, HGA-1, was also identified. Absorptions revealed a quite different distribution for HGA-1 than HGA-3, this antigen being detected on monocytes and myeloblasts as well as on mature granulocytes. Independent segregation of the three loci from HLA, from the NA-NB and the 5a-5b antigens, and from themselves was confirmed in informative families.Finally, it seems likely that other antigens will be identified because several other sera that react with both monocytes and granulocytes have been detected.

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Section: Resultsmentioning

confidence: 99%

New Granulocyte Antigens Demonstrated by Microgranulocytotoxicity Assay

Thompson¹,

Overlin²,

Herbick³

et al. 1980

J. Clin. Invest.

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“…All tests were performed using 12 consecutive two-fold dilutions of the test plasma in 0.9% NaCl; the same dilutions of plasma obtained from healthy donors were used as controls. The morphological identity of the cells agglutinated by the antibody was determined by microscopic examination of incubation mixtures that had been dried on microscope slides and prepared by Wright's stain (3). Techniques for the preparation of various blood and tissue cells and their application in antibody absorption procedures were those previously reported (3).…”

Section: Experi Mentalsmentioning

confidence: 99%

“…The morphological identity of the cells agglutinated by the antibody was determined by microscopic examination of incubation mixtures that had been dried on microscope slides and prepared by Wright's stain (3). Techniques for the preparation of various blood and tissue cells and their application in antibody absorption procedures were those previously reported (3). The lymphocytotoxicity test was performed according to the method of Mittal, Mickey, Singal, and Terasaki (13).…”

Section: Experi Mentalsmentioning

confidence: 99%

“…Methods for DEAE-cellulose column chromatography and 2-mercaptoethanol treatment of the antibody are described in an earlier report (3).…”

Section: Experi Mentalsmentioning

confidence: 99%

“…Neonatal neutropenia is a disorder of the newborn resulting from maternal isoimmunization by fetal neutrophils (1)(2)(3). Although identification of the immunological basis of this condition has had important clinical implications, characterization of the antibodies involved and the corresponding antigens has wider application.…”

Section: Introductionmentioning

confidence: 99%

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NB1, a new neutrophil-specific antigen involved in the pathogenesis of neonatal neutropenia

Lalezari¹,

Murphy²,

Allen³

1971

J. Clin. Invest.

144

110

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A B S T R A C T A new human antigen is reported which is present only on blood neutrophils. A neutrophil-specific antigen, designated NAI, has previously been identified in two unrelated families, and was shown to be involved in fetomaternal incompatibility and the development of isoimmune neonatal neutropenia in five newborns. In the present paper, a second antigen, designated NB1, is identified in four families with seven affected children. Antibodies that react with this second antigen are shown to produce selective agglutination of neutrophils but not other blood cells. They are neither absorbed by cells prepared from solid tissues nor by non-neutrophilic blood cells.By family and population studies, NB is shown to be distinct from NA, representing an independent genetic locus. 68% of the New York population are homozygous for NB1, 29% heterozygous, and 3% negative. The NB locus is shown to be independent from those of HL-A and other known leukocyte antigens. No evidence for linkage between NA, NB, and red cell antigens was obtained.

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Granulocytotoxic antibodies in a patient with propylthiouracil-induced agranulocytosis

Guffy¹

1984

Archives of Internal Medicine

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Agranulocytosis developed in a patient who was receiving propylthiouracil. Using a microgranulocytotoxicity assay, serum taken from the patient was shown to be strongly granulocytotoxic when tested against the patients granulocytes and those obtained from two of eight normal subjects. Tests for granulocyte agglutinins and for lymphocytotoxicity were negative. Granulocytotoxic activity decreased as the patient's peripheral granulocyte count recovered. Cytotoxicity was shown to be mediated by a complement-dependent IgM antibody.

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An isologous antigen-antibody reaction with human neutrophiles, related to neonatal neutropenia.

Cited by 86 publications

References 16 publications

New Granulocyte Antigens Demonstrated by Microgranulocytotoxicity Assay

New Granulocyte Antigens Demonstrated by Microgranulocytotoxicity Assay

NB1, a new neutrophil-specific antigen involved in the pathogenesis of neonatal neutropenia

Granulocytotoxic antibodies in a patient with propylthiouracil-induced agranulocytosis

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