An iTRAQ-Based Quantitative Proteomic Analysis of Plasma Proteins in Preterm Newborns With Retinopathy of Prematurity

Zasada, Magdalena; Suski, Maciej; Bokiniec, Renata; Szwarc-Duma, Monika; Borszewska−Kornacka, Maria Katarzyna; Madej, Józef; Bujak-Giżycka, Beata; Madetko‐Talowska, Anna; Revhaug, Cecilie; Baumbusch, Lars Oliver; Saugstad, Ola Didrik; Pietrzyk, Jacek J; Kwinta, Przemko

doi:10.1167/iovs.18-24914

Cited by 12 publications

(11 citation statements)

References 48 publications

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“…Previous studies, including ours, also noted (i) elevated cord plasma levels of IL-6, C3a, and C5a, and (ii) events in the intrauterine environment that trigger spontaneous preterm delivery are significantly associated with an increased risk of ROP progression. 13 , 32 , 33 Taken together, these findings suggest that pathophysiologic events that predispose preterm neonates to ROP begin before delivery and that therapeutic strategies to decrease the risk of ROP may need to be implemented during pregnancy (e.g., specific treatment with antibiotics, anti-inflammatory drugs, and/or antiangiogenic drugs). Additionally, our findings strongly support the theory of Lee and Dammann 34 regarding the important etiological role of antenatal factors, especially prenatal and perinatal infection/inflammation, in ROP.…”

Section: Discussionmentioning

confidence: 95%

Inflammatory and Angiogenic Mediators in Amniotic Fluid Are Associated With the Development of Retinopathy of Prematurity in Preterm Infants

Woo

Park

Hong

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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Purpose To investigate whether elevated levels of inflammatory/angiogenic and growth mediators in amniotic fluid (AF) and the presence of intra-amniotic infection are associated with the occurrence and progression of retinopathy of prematurity (ROP) in preterm infants. Methods This retrospective cohort study included 175 premature singleton infants who were born between 23+0 and 32+0 weeks. AF obtained via amniocentesis was cultured, and endoglin, endostatin, insulin-like growth factor-binding protein (IGFBP)-2, IGFBP-3, IGFBP-4, IL-6, IL-8, matrix metalloproteinase-8, matrix metalloproteinase-9, and vascular endothelial growth factor receptor-1 levels were assayed by ELISA. The primary outcome measures included the occurrence of any stage ROP, severe ROP (stage ≥3), and vision-threatening type 1 ROP requiring treatment. Results Multiple logistic regression analyses revealed that there are significant associations between elevated AF endoglin levels and ROP occurrence; between elevated AF endoglin, endostatin, and IGFBP-2 levels and severe ROP; and between high AF endoglin, IL-6, and IL-8 levels and vision-threatening ROP requiring treatment, after adjusting for potential postnatal confounders. Using stepwise regression analyses, antenatal prediction models based on these AF biomarkers and prenatal factors were developed for the ROP outcomes, which had good discriminatory power (area under the curves, 0.731–0.863). However, we found that intra-amniotic infection is not associated with ROP occurrence and progression. Conclusions Elevated levels of inflammatory (IL-6 and IL-8) and angiogenic (endoglin and IGFBP-2) mediators in the AF, but not the presence of intra-amniotic infection, are independently associated with the occurrence and progression of ROP in preterm infants. These findings suggest that the pathophysiologic events that predispose preterm neonates to ROP may begin before delivery.

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Section: Discussionmentioning

confidence: 95%

Inflammatory and Angiogenic Mediators in Amniotic Fluid Are Associated With the Development of Retinopathy of Prematurity in Preterm Infants

Woo

Park

Hong

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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“…A second blood sample (peripheral venous blood) was taken at 36 PMA. The plasma samples were further used for Combinatorial Peptide Ligand Libraries- isobaric Tag for Relative and Absolute Quantitation (CPLL-iTRAQ) quantitative analysis as previously described [8, 10].…”

Section: Methodsmentioning

confidence: 99%

“…The articles presented that proteome differences are highly gestational age-dependent. Recently, we also published data describing the differences in plasma protein abundances in prematurely born infants with and without retinopathy of prematurity (ROP) [10].…”

Section: Introductionmentioning

confidence: 99%

Comparative two time-point proteome analysis of the plasma from preterm infants with and without bronchopulmonary dysplasia

et al. 2019

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Background In this study, we aimed to analyze differences in plasma protein abundances between infants with and without bronchopulmonary dysplasia (BPD), to add new insights into a better understanding of the pathogenesis of this disease. Methods Cord and peripheral blood of neonates (≤ 30 weeks gestational age) was drawn at birth and at the 36th postmenstrual week (36 PMA), respectively. Blood samples were retrospectively subdivided into BPD(+) and BPD(−) groups, according to the development of BPD. Results Children with BPD were characterized by decreased afamin, gelsolin and carboxypeptidase N subunit 2 levels in cord blood, and decreased galectin-3 binding protein and hemoglobin subunit gamma-1 levels, as well as an increased serotransferrin abundance in plasma at the 36 PMA. Conclusions BPD development is associated with the plasma proteome changes in preterm infants, adding further evidence for the possible involvement of disturbances in vitamin E availability and impaired immunological processes in the progression of prematurity pulmonary complications. Moreover, it also points to the differences in proteins related to infection resistance and maintaining an adequate level of hematocrit in infants diagnosed with BPD.

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“…Within the last decade proteomics has enabled researchers to identify predictive biomarkers of NEC in preterm infants using buccal swabs [ 9 ]. More specifically, plasma proteomics has previously identified proteins that may play a role in the development of retinopathy of prematurity [ 10 ]. However, to date there has been limited research into plasma protein biomarkers in predicting other outcomes in preterm infants.…”

Section: Introductionmentioning

confidence: 99%

The use of proteomics for blood biomarker research in premature infants: a scoping review

et al. 2021

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Over the last decade, the use of proteomics in the setting of prematurity has increased and has enabled researchers to successfully identify biomarkers for an array of associated morbidities. The objective of this scoping review was to identify the existing literature, as well as any knowledge gaps related to proteomic biomarker discoveries in the setting of prematurity. A scoping review was conducted using PubMed, Embase and Medline databases following the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines. The study selection process yielded a total of 700 records, of which 13 studies were included in this review. Most studies used a tandem Mass Spectrometry (MS/MS) proteomics approach to identify key biomarkers. The corresponding studies identified proteins associated with retinopathy of prematurity (ROP), bronchopulmonary dysplasia (BPD), necrotising enterocolitis (NEC), late onset sepsis (LOS) and gestational age. This scoping review demonstrates the limited use of proteomics to identify biomarkers associated with severe complications of prematurity. Further research is warranted to identify biomarkers of other important morbidities associated with prematurity, such as intraventricular haemorrhage (IVH) and cerebral palsy, and to investigate the mechanisms associated with these outcomes.

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An iTRAQ-Based Quantitative Proteomic Analysis of Plasma Proteins in Preterm Newborns With Retinopathy of Prematurity

Cited by 12 publications

References 48 publications

Inflammatory and Angiogenic Mediators in Amniotic Fluid Are Associated With the Development of Retinopathy of Prematurity in Preterm Infants

Inflammatory and Angiogenic Mediators in Amniotic Fluid Are Associated With the Development of Retinopathy of Prematurity in Preterm Infants

Comparative two time-point proteome analysis of the plasma from preterm infants with and without bronchopulmonary dysplasia

The use of proteomics for blood biomarker research in premature infants: a scoping review

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