An LC–MS method for simultaneous determination of five iridoids from Zhi-zi-chi Decoction in rat brain microdialysates and tissue homogenates: Towards an in depth study for its antidepressive activity

Qu, Kankan; Zhao, Longshan; Luo, Xinyi; Zhang, Chenning; Hou, Pengyi; Bi, Kaishun; Chen, Xiaohui

doi:10.1016/j.jchromb.2014.03.032

Cited by 26 publications

(16 citation statements)

References 22 publications

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“…On the other hand, verapamil influenced the transportation of geniposide, while EDTA did not which indicated that the absorption of geniposide in vivo may involve the active efflux mechanism mediated by P-glycoprotein [113]. The discovery of gardenoside in the brain suggests that it can pass through the blood-brain barrier for therapeutic purposes [114]. The pharmacokinetics of geniposide in rats with type 2 diabetic rats had higher C max , larger area under curve (AUC), longer T max , lower clearance rate (CL), and shorter mean residence time (MRT) (p < 0.05), compared to the healthy rats.…”

Section: Iridoid Glycosidesmentioning

confidence: 99%

Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics

et al. 2020

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Iridoids are a class of active compounds that widely exist in the plant kingdom. In recent years, with advances in phytochemical research, many compounds with novel structure and outstanding activity have been identified. Iridoid compounds have been confirmed to mainly exist as the prototype and aglycone and Ι and II metabolites, by biological transformation. These metabolites have been shown to have neuroprotective, hepatoprotective, anti-inflammatory, antitumor, hypoglycemic, and hypolipidemic activities. This review summarizes the new structures and activities of iridoids identified locally and globally, and explains their pharmacokinetics from the aspects of absorption, distribution, metabolism, and excretion according to the differences in their structures, thus providing a theoretical basis for further rational development and utilization of iridoids and their metabolites.

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Section: Iridoid Glycosidesmentioning

confidence: 99%

Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics

et al. 2020

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“…Various chromatographic methods have been published for the determination of GE or GP in combination with other compounds in the biological matrix using HPLC‐UV or LC–MS/MS (Chen et al, ; Cheng, Lin, Lin, & Tsai, ; He et al, ; Hu et al, ; Huang et al, ; Peng et al, ; Qu et al, , ; Wang et al, ). Some methods have been established for simultaneous quantification of GE and GP.…”

Section: Introductionmentioning

confidence: 99%

A sensitive LC–MS/MS method for simultaneous quantification of geniposide and its active metabolite genipin in rat plasma and its application to a pharmacokinetic study

Shi

Pan

et al. 2017

Biomedical Chromatography

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Genipin (GP), an active metabolite of geniposide (GE), exhibits more potent pharmacological effects than its parent compound. In this paper, a sensitive LC-MS/MS method was developed and fully validated for the simultaneous determination of GE and GP in rat plasma. We found that GP degraded rapidly in rat plasma at room temperature as a result of irreversible binding with the endogenous nucleophiles in plasma. GP was stable when the sample's pH was ≤4.0. The degradation of GP in rat plasma was well prevented by immediate addition of 5% glacial acetic acid to the freshly collected plasma. The detection was performed on a tandem mass spectrometer coupled with electrospray ionization source in negative mode. Quantification was conducted by multiple reaction monitoring of the transitions [M + CH COO] m/z 447.3 → 225.3 for GE and [M - H] m/z 225.2 → 123.1 for GP. The method exhibited high sensitivity (LLOQ 1 ng/mL for GE and 0.2 ng/mL for GP) by selecting the acetate adduct ions as the precursor ions for GE. The robust developed method was successfully applied to a pharmacokinetic study in rats after oral administration of GE.

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“…In TCM, herb compounds containing G. jasminoides fruits, such as Zhi-zi-chi preparations [60], the Yueju pill and others, has been widely used for the treatment of depression in East Asian countries for hundreds of years [61]. Recently, the mechanism of action of these herb medicines has been gradual uncovered by pharmacological studies.…”

Section: Antidepressant Effect Of Genipinmentioning

confidence: 99%

“…Further research showed that iridoid compounds, geniposide and genipin, are the major bioactive ingredients for antidepressant activity in these herb compounds containing G. jasminoides fruits. The iridoid compounds could pass through the blood brain barrier (BBB) and distribute in the hippocampus, hypothalamus, premotor cortex, striatum, oblongata and cerebellum [60]. In addition, iridoid compound show significant selective MAO-B inhibition and are more potent than the other isolated compounds.…”

Section: Jasminoides Fruits Can Inhibit Mao-a/b and Dopamine β-Hydroxmentioning

confidence: 99%

Therapeutic Potential of Genipin in Central Neurodegenerative Diseases

Hölscher

2016

CNS Drugs

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Central neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD), are one of the biggest health problems worldwide. Currently, there is no cure for these diseases. The Gardenia jasminoides fruit is a common herbal medicine in traditional Chinese medicine (TCM), and a variety of preparations are used as treatments for central nervous system (CNS) diseases. Pharmacokinetic studies suggest genipin is one of the main effective ingredients of G. jasminoides fruit extract (GFE). Accumulated research data show that genipin possesses a range of key pharmacological properties, such as anti-inflammatory, neuroprotective, neurogenic, antidiabetic, and antidepressant effects. Thus, genipin shows therapeutic potential for central neurodegenerative diseases. We review the pharmacological actions of genipin for the treatment of neurodegenerative diseases of the CNS. We also describe the potential mechanisms underlying these effects.

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An LC–MS method for simultaneous determination of five iridoids from Zhi-zi-chi Decoction in rat brain microdialysates and tissue homogenates: Towards an in depth study for its antidepressive activity

Cited by 26 publications

References 22 publications

Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics

Iridoids: Research Advances in Their Phytochemistry, Biological Activities, and Pharmacokinetics

A sensitive LC–MS/MS method for simultaneous quantification of geniposide and its active metabolite genipin in rat plasma and its application to a pharmacokinetic study

Therapeutic Potential of Genipin in Central Neurodegenerative Diseases

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