An LC–MS–MS method for the determination of cyclizine in human serum

Quantification of cyclizine and norcyclizine in human plasma by liquid chromatography–tandem mass spectrometry (LC–MS/MS)

“…We were unable to locate any LC-MS/MS assays in the literature involving both cyclizine and norcyclizine. Cyclizine, but not norcyclizine, has been determined in human plasma by LC-MS/MS as reported by the Walker and Kanfer group [10], and an LC-MS/MS antihistamine screening assay, which includes cyclizine, has been described [11]. As no existing methods were suitable for our purposes, the aim of the current work was to develop and validate a rapid and simple LC-MS/MS assay for simultaneous determination of cyclizine and norcyclizine concentrations in human plasma to be used for a clinical study.…”

“…2). Positive mode ionisation and the MRM transition 267/167 for cyclizine have also been used in the two previously reported LC-MS/MS assays for cyclizine [10,11].…”

“…Processed samples stored in the autosampler at 15 • C for 56 h deviated less than 7% from freshly prepared samples. In the literature, cyclizine has been reported to be stable in serum for up to 4 weeks at −10 • C and 4 • C [8,10] and able to tolerate three freeze-thaw cycles [10]. No data regarding norcyclizine stability in plasma could be found, but norcyclizine was reported to be stable in urine for 4 weeks at −10 • C and for 2 weeks at 4 • C [8].…”

Ultrasensitive flow-injection electrochemical method for determination of histamine in tuna fish samples

“…The method was validated with respect to parameters including linearity, limit of quantitation (LOQ), limit of detection (LOD), precision, accuracy, ruggedness/robustness, recovery and selectivity (Mohammadi, Kanfer, Sewram, & Walker, 2005;Mohammadi et al, 2007).…”

Application of Adsorptive Voltammetry for the Detection of Sub-nano Molar Cyclizine in Biological Fluids and Tablets Using Fast Fourier Transform Continuous Cyclic Voltammetry in a Flowing System

“…1,2 Colorimetry, 3 potentiometry 4 second derivative UV spectrophotometry, 5 HPLC, [6][7][8] solid phase extraction, 9 GC, 10 micellar RPLC, 11 LC-MS, 12 LC-MS-MS 13 and CZE 14 are applied for determination of cyclizine. The lack of the requisite sensitivity and/or selectivity, large sample volumes and complex procedures are the limitations of some of these methods.…”