An N‐methylpolyvinylpyridinium cationic polymer for capillary coating in electrophoresis of proteins and peptides

Sebastiano, Roberto; Mendieta, Martha Elena; Contiello, Nunzia; Citterio, Attilio; Righetti, Pier Giorgio

doi:10.1002/elps.200800672

Cited by 16 publications

(18 citation statements)

References 36 publications

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“…Barium carbonate was prepared by precipitating barium hydroxide with carbon dioxide; the precipitate was filtered, washed and dried. PVPy-Me iodine was synthesized according to the previously described procedure [14]. SAM and MTA were obtained from Omniabios S.r.L.…”

Section: Chemicals and Materialsmentioning

confidence: 99%

“…In the second approach, we employed a novel cationic polymeric coating, consisting of a N-methylpolyvinylpyridinium (PVPy-Me) quaternary ion. This coating, combined with an isoelectric, aspartic acid as BGE, had been shown by us to offer excellent separations of low-to high-Mr proteins, of low-to high-pI values, coupled to very high run-to-run repeatability [14] and full ESI MS compatibility [15].…”

Section: Introductionmentioning

confidence: 98%

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Analysis of trace degradation products (decarboxylated diastereoisomers) of S‐adenosylmethionine by electrophoresis in capillaries with cationic coatings (N‐methylpolyvinylpyridinium or divalent barium)

et al. 2010

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Commercial preparations of S-adenosylmethionine (SAM) when analyzed in uncoated capillaries show a minute impurity believed to be decarboxylated (dc) SAM. By using two types of cationic coatings, thus reducing the electro-endo-osmotic flow (EOF), it was possible to separate this impurity into two diastereoisomers of dcSAM. The coatings evaluated for this purpose were: (i) N-methylpolyvinylpyridinium, used under reversed EOF at acidic conditions (pH 4.0) and (ii) deposition of divalent barium at alkaline pH values (pH 9.4), providing reduced EOF. Under these conditions, it was possible to separate this impurity into two diastereoisomers, which by chemical synthesis were indeed proven to be dcSAM. It was further demonstrated that, in the alkylation of 5'-methylthioadenosine by 3-bromopropylamine in bromidric acid to dcSAM, another minute impurity was present, proven, via mass spectrometry, to consist of S-(5'-adenosyl)-3-thiopropylamine (decarboxylated and demethylated (dc-SAH)). The LOD for the two dcSAM diastereoisomers was assessed as 17.5 μg/mL and their LOQ as 25.5 μg/mL. By the barium-based protocol it was possible to quantify the dcSAM, present in a commercial sample of SAM, as a 0.1% impurity.

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Section: Chemicals and Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Analysis of trace degradation products (decarboxylated diastereoisomers) of S‐adenosylmethionine by electrophoresis in capillaries with cationic coatings (N‐methylpolyvinylpyridinium or divalent barium)

et al. 2010

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“…It possesses the following beneficial properties: shortened analysis time, improved efficiency, reduced sample consumption, and easier automation [1][2][3]. It possesses the following beneficial properties: shortened analysis time, improved efficiency, reduced sample consumption, and easier automation [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Polysiloxane graft poly(2‐vinylpyridine) copolymer‐based capillary coating for separation of dsDNA

2016

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Polyether-modified polysiloxane graft poly(2-vinylpyridine) (PMP-g-P2VP) copolymer, which was confirmed by NMR spectra and GPC, was synthesized for separation of dsDNA by capillary electrophoresis. EOF measurement results showed that the synthesized copolymer-coated capillaries in this report could suppress EOF effectively compared to the bare fused-silica capillaries. The effectiveness of PMP-g-P2VP polymer as a media for DNA analysis was discussed. Significant improvement in resolution of dsDNA analysis was demonstrated compared to the conventional polymer matrix. Results showed that 100-bp DNA ladder samples achieved separation in a 30 cm effective capillary length at 2.0% concentration by using this copolymer. Finally, the relationships between morphologies of copolymer in glass surface and the separation efficiencies were studied by scanning electron microscope (SEM).

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“…Nowadays, increasing attention has been shifted toward coating the capillary wall. As it has been defined by Lucy [10], coating can be broadly categorized as: covalently linked polymeric coating [11][12][13][14][15][16]; physically adsorbed polymer coatings [17][18][19][20][21]; or small molecule additives [22,23]. Among different strategies to coat the capillary surface and control the wall-protein interactions, polymer used as separation coating attracts increasing attention due to their unique properties.…”

Section: Introductionmentioning

confidence: 99%

A novel PEG coating immobilized onto capillary through polydopamine coating for separation of proteins in CE

Zeng

Zhou

et al. 2010

Electrophoresis

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The antifouling PEG-immobilized capillary was introduced for the protein separation in CE through mussel adhesive protein inspired polydopamine coating for the first time. The polydopamine, formed by spontaneous oxidative polymerization of dopamine at alkaline in the inner surface of capillary, was exploited to immobilize amine-functionalized PEG onto the capillary surface. During the process, polydopamine-graft-PEG copolymer was formed via Michael addition or Schiff base reactions. The polymer coating was observed using X-ray photoelectron spectroscopy and SEM. And both of them indicated the formation of the polymer coating. A comparative study of EOF showed that the novel coating could provide effective suppression of EOF and minimized adsorption of proteins. As a consequence, fast and efficient separations of three proteins such as lysozyme, cytochrome c, and ribonuclease A were obtained within a broad pH range. Furthermore, the long-term stability of polydopamine-graft-PEG coating in consecutive protein separation runs and the high separation efficiency proved that this novel coating was capable of minimizing protein adsorption during the capillary separation. The successful capillary performance also was demonstrated in the separation of protein mixture and milk powder samples at acidic pH.

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An N‐methylpolyvinylpyridinium cationic polymer for capillary coating in electrophoresis of proteins and peptides

Cited by 16 publications

References 36 publications

Analysis of trace degradation products (decarboxylated diastereoisomers) of S‐adenosylmethionine by electrophoresis in capillaries with cationic coatings (N‐methylpolyvinylpyridinium or divalent barium)

Analysis of trace degradation products (decarboxylated diastereoisomers) of S‐adenosylmethionine by electrophoresis in capillaries with cationic coatings (N‐methylpolyvinylpyridinium or divalent barium)

Polysiloxane graft poly(2‐vinylpyridine) copolymer‐based capillary coating for separation of dsDNA

A novel PEG coating immobilized onto capillary through polydopamine coating for separation of proteins in CE

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