An N-Myristoylated Globin with a Redox-Sensing Function That Regulates the Defecation Cycle in Caenorhabditis elegans

Tilleman, Lesley; Henau, Sasha De; Pauwels, M.; Nagy, Nóra Veronika; Pintelon, Isabel; Braeckman, Bart P.; Wael, Karolien De; Doorslaer, Sabine Van; Adriaensen, Dirk; Timmermans, Jean‐Pierre; Moëns, Luc; Dewilde, Sylvia

doi:10.1371/journal.pone.0048768

Cited by 15 publications

(13 citation statements)

References 94 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The remaining SRP genes include a Myb-like/SANT-like transcription factor (Sro94_g048900), a metallopeptidase (Sro489_g153380), a gene encoding a transmembrane protein containing an EF-hand domain (Sro1_g000260) and a globin (Sro2353_g324460) ( Figure 4C). The latter gene is especially interesting as, next to their oxygen binding capabilities, globins are increasingly implicated in redox signaling 65,66 . When exploring ROS signaling related genes, the conventional DE analysis uncovered an NADPH oxidase (NOX, Sro1572_g283390) with a pronounced MT+ specific response to SIP (Figure 3), catalyzing extracellular production of superoxide anions by moving an electron from NADPH through the plasma membrane to molecular oxygen.…”

Section: Resultsmentioning

confidence: 99%

Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatomSeminavis robusta

Bilcke

Berge

Decker

et al. 2020

Preprint

View full text Add to dashboard Cite

Sexual reproduction is a fundamental phase in the life cycle of most diatoms. Despite its role as a source of genetic variation, it is rarely reported in nature and its molecular foundations remain largely unknown.Here, we integrate independent transcriptomic datasets, in order to prioritize genes responding to sex inducing pheromones (SIPs) in the pennate diatom Seminavis robusta. We observe marked gene expression changes associated with SIP treatment in both mating types, including an inhibition of S-phase progression, chloroplast division, mitosis and cell wall formation. Meanwhile, meiotic genes are upregulated in response to SIP, including a sexually induced diatom specific cyclin (dsCyc). Our data further suggest an important role for reactive oxygen species, energy metabolism and cGMP signaling during the early stages of sexual reproduction. In addition, we identify several genes with a mating type specific response to SIP, and link their expression pattern with physiological responses such as the production of the attraction pheromone diproline and mate-searching behaviour in MT+. Combined, our results provide a model for early sexual reproduction in pennate diatoms and significantly expand the suite of target genes to detect sexual reproduction events in natural diatom populations.

show abstract

Section: Resultsmentioning

confidence: 99%

Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatomSeminavis robusta

Bilcke

Berge

Decker

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Although, palmitoylation occurs mainly close to the N-terminus of proteins, it has also been observed in other parts of proteins 87 , 88 . Globin proteins associated with the membrane have been identified in some bacteria 44 - 47 , in the nematode Caenorhabditis elegans 89 , in the shore crab Carcinus maenas 48 , and recently also in vertebrates 49 , 50 . However, there is no evidence that these globins share common ancestry suggesting that membrane-associated globins arouse independently several times in the course of evolution.…”

Section: Discussionmentioning

confidence: 99%

Amoebozoa Possess Lineage-Specific Globin Gene Repertoires Gained by Individual Horizontal Gene Transfers

Dröge¹,

Buczek

Suzuki³

et al. 2014

Int. J. Biol. Sci.

View full text Add to dashboard Cite

The Amoebozoa represent a clade of unicellular amoeboid organisms that display a wide variety of lifestyles, including free-living and parasitic species. For example, the social amoeba Dictyostelium discoideum has the ability to aggregate into a multicellular fruiting body upon starvation, while the pathogenic amoeba Entamoeba histolytica is a parasite of humans. Globins are small heme proteins that are present in almost all extant organisms. Although several genomes of amoebozoan species have been sequenced, little is known about the phyletic distribution of globin genes within this phylum. Only two flavohemoglobins (FHbs) of D. discoideum have been reported and characterized previously while the genomes of Entamoeba species are apparently devoid of globin genes. We investigated eleven amoebozoan species for the presence of globin genes by genomic and phylogenetic in silico analyses. Additional FHb genes were identified in the genomes of four social amoebas and the true slime mold Physarum polycephalum. Moreover, a single-domain globin (SDFgb) of Hartmannella vermiformis, as well as two truncated hemoglobins (trHbs) of Acanthamoeba castellanii were identified. Phylogenetic evidence suggests that these globin genes were independently acquired via horizontal gene transfer from some ancestral bacteria. Furthermore, the phylogenetic tree of amoebozoan FHbs indicates that they do not share a common ancestry and that a transfer of FHbs from bacteria to amoeba occurred multiple times.

show abstract

“…Besides GLB-12, 2 other globins appear to play a role in redox signaling, namely GLB-6 and GLB-26. 34,35 …”

Section: Globins Can Actively Drive Redox Signalingmentioning

confidence: 99%

“…Previous results of our group indicated that GLB-26 has a role in redox signaling. 34 GLB-26 is exclusively expressed in the stomato-intestinal and anal depressor muscle cell and in the head mesodermal cell, all of which appear to be associated with the regulation of the defecation cycle. We observed that the defecation cycle is normal in a glb-26 knockout strain, but is differentially affected in WT and glb-26 knockout worms when they are exposed to high concentrations of a ROS producing compound.…”

Section: Globins Can Actively Drive Redox Signalingmentioning

confidence: 99%

Globin-based redox signaling

Henau

Braeckman

2016

Worm

Self Cite

View full text Add to dashboard Cite

In recent years, moderate levels of reactive oxygen species (ROS) have become recognized as signaling cues that participate at all levels of cellular organization. Globins, with their redox-active heme iron and ubiquitous presence, seem ideally suited to participate in ROS metabolism. Here we comment on our recent findings that show the participation of a globin, GLB-12, in a redox signaling pathway in Caenorhabditis elegans. We found that GLB-12 produces superoxide, a type of ROS, after which this is converted to what appears to be a hydrogen peroxide gradient over the plasma membrane by the activity of intracellular and extracellular superoxide dismutases. In the first part, we discuss in more detail the different regulatory mechanisms that increase the effectiveness of this redox signal. In the second part, we comment on how specific structural and biochemical properties allow this globin to perform redox reactions. Interestingly, these properties are also observed in 2 other C. elegans globins that appear to be involved in redox biology. We therefore hypothesize that globins involved in redox signaling display similar structural and biochemical characteristics and propose that a subgroup of globins can be added to the group of proteins that play a vital role in redox signaling.

show abstract

An N-Myristoylated Globin with a Redox-Sensing Function That Regulates the Defecation Cycle in Caenorhabditis elegans

Cited by 15 publications

References 94 publications

Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatomSeminavis robusta

Mating type specific transcriptomic response to sex inducing pheromone in the pennate diatomSeminavis robusta

Amoebozoa Possess Lineage-Specific Globin Gene Repertoires Gained by Individual Horizontal Gene Transfers

Globin-based redox signaling

Contact Info

Product

Resources

About