An o-aminobenzoic acid film-based immunoelectrode for detection of the cardiac troponin T in human serum

Mattos, Alessandra B.; Freitas, Tatianny A.; Kubota, Lauro T.; Dutra, Rosa F.

doi:10.1016/j.bej.2012.12.005

Cited by 28 publications

(10 citation statements)

References 36 publications

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“…1iv compares the analytical response of the immunosensor using square wave voltammograms of the quantum dots on bare and graphene-modified electrodes. The peak current (at −0.8 V) was found to be three times greater with the graphene-modified 4.69 μA ng −1 mL −2 CV = 5.6%; n = 5 r = 0.992 [75] (continued on next page) [96] (continued on next page) electrode than with the bare electrode (only with quantum dots), exhibiting an acceptable response for further biosensing of PSA. According to Yang et al [55], graphene sheets offer a very large surface area for the immobilization of several quantum dots, thus increasing the sensitivity of the immunosensor.…”

Section: Cancer Biomarkersmentioning

confidence: 98%

“…Increased levels of cTnT (higher than 0.3 ng mL −1 ) are highly specific to cardiac injury and are significantly associated with an increased risk of re-infarction and death, which facilities early diagnosis [93]. As shown in Table 2, Fonseca et al [93], Gomes-Filho et al [94], Silva et al [95] and Mattos et al [96] developed immunosensors based on gold nanoparticles, carboxylated CNTs, amine-functionalized CNTs and o-aminobenzoic films, respectively, for immobilizing antibodies specific to cTnT, with an LOD ranging from 0.0015 to 0.033 ng mL −1 . All immunosensors were used for serum samples with high sensitivity.…”

Section: Cardiac Biomarkersmentioning

confidence: 99%

“…Troponins are the most specific and sensitive biomarkers of myocardial cell injury. They, they have been used to test for acute myocardial infarction [96]. Troponins such as cTnT and cTnI can control the calcium-mediated interactions between actin and myosin in cardiac and skeletal muscles; cTnT is expressed in skeletal muscle to a lesser extent, whereas cTnI has not been identified outside of the myocardium [99].…”

Section: Cardiac Biomarkersmentioning

confidence: 99%

See 2 more Smart Citations

Critical overview on the application of sensors and biosensors for clinical analysis

Justino

Duarte

Rocha-Santos

2016

TrAC Trends in Analytical Chemistry

133

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A B S T R A C TSensors and biosensors have been increasingly used for clinical analysis due to their miniaturization and portability, allowing the construction of diagnostic devices for point-of-care testing. This paper presents an up-to-date overview and comparison of the analytical performance of sensors and biosensors recently used in clinical analysis. This includes cancer and cardiac biomarkers, hormones, biomolecules, neurotransmitters, bacteria, virus and cancer cells, along with related significant advances since 2011. Some methods of enhancing the analytical performance of sensors and biosensors through their figures of merit are also discussed.

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Section: Cancer Biomarkersmentioning

confidence: 98%

Section: Cardiac Biomarkersmentioning

confidence: 99%

Section: Cardiac Biomarkersmentioning

confidence: 99%

See 1 more Smart Citation

Critical overview on the application of sensors and biosensors for clinical analysis

Justino

Duarte

Rocha-Santos

2016

TrAC Trends in Analytical Chemistry

133

View full text Add to dashboard Cite

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“…Mattos et al. 34 have reported the use of a carboxylic film obtained through electropolymerization of o ‐aminobenzoic acid ( o ‐ABA) on a glassy carbon electrode (GCE) to covalently bind antibodies against cTnT by their amino reactive groups in the side chains onto the electrode surface. Chronoamperometric detection of cTnT was carried out at − 0.05 V vs. Ag/AgCl using peroxidase conjugated mouse monoclonal antibodies against cTnT and H 2 O 2 as the enzymatic substrate.…”

Section: Electrochemical Biosensors For Cardiovascular Markersmentioning

confidence: 99%

Electrochemical Biosensors for the Determination of Cardiovascular Markers: a Review

2014

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Nowadays there is a growing interest in the development of devices for the fast and reliable detection and quantification of biomarkers associated with cardiovascular diseases (CVDs). The main objective is not only an early diagnosis of these diseases but also predicting the risk that an individual can suffer CVD. Electrochemical biosensors can play a key role in the development of low cost, portable and rapid sensing platforms for this purpose. This review discusses critically the state of the art in the development, applications and potential suitability of electrochemical biosensors for the determination of cardiovascular biomarkers as well as the required characteristics that these sensors should fulfill to be finally accepted in the clinical community.

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“…In this regard, the biosensor development for selective troponin detection becomes a valuable guide for diagnosis of lesions of myocardial cells. [41][42][43][44][45] In this work, we investigated the formation of a new modified electrode based on electropolymerization of 3-aminophenol on surface of fluorine doped tin oxide (FTO). The electrochemical and morphologic properties of the modified electrode were studied, with subsequent application in the biosensor development for detection of the cardiac marker troponin T, following two ways: label-free electrochemical transduction and optical probe by labelling with quantum dots.…”

Section: Introductionmentioning

confidence: 99%

Bioelectrode Applied to Diagnosis of Cardiac Disease

Lp¹,

Ferreira²,

Af³

et al. 2014

j nanosci nanotechnol

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This paper describes the assembly of a bioelectrode based on poly(3-aminophenol) and anti-troponin T antibody for recognition of troponin T, which is a specific biomarker for diagnosis of acute myocardial infarction. This disease causes loss of cellular components, allowing the output of molecules such as troponin T. This proteic component acts as biomarker for diagnosis of acute myocardial infarction due to their high sensitivity and specificity. Poly(3-aminophenol) was electrodeposited onto fluorine doped tin oxide (FTO) coated glass and characterized by spectroscopic methods (UV-Visible, fluorescence, infrared), electrochemical methods (cyclic voltammetry and electrochemical impedance spectroscopy) and morphological methods (laser interferometry, field emission scanning electronic microscopy, and atomic force microscopy). UV/Vis analysis indicated that poly(3-aminophenol) presents extension of conjugation, in according with fluorescence studies. Electrochemical studies indicated that poly(3-aminophenol) electrodeposited in FTO is a material with passivating characteristics for anions and capacity of retaining cationic compounds. Laser interferometry showed that poly(3-aminophenol) covers the FTO surface with a thickness off 375 ± 75 nm. Surface images by FE-SEM and AFM have shown a full coverage on the FTO by the polymer film. The incorporation of anti-troponin T antibody on FTO electrode modified with poly(3-aminophenol) allowed effective and selective detection of cardiac biomarker troponin T, by electrochemical impedance spectroscopy (label free) and by photoluminescence, based on CdSe/ZnS quantum dots. This research shows the step by step assembly of the bioelectrode, used for detection of troponin T by impedimetric and fluorescence methods, opening the opportunity for its use in the diagnosis of others diseases.

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An o-aminobenzoic acid film-based immunoelectrode for detection of the cardiac troponin T in human serum

Cited by 28 publications

References 36 publications

Critical overview on the application of sensors and biosensors for clinical analysis

Critical overview on the application of sensors and biosensors for clinical analysis

Electrochemical Biosensors for the Determination of Cardiovascular Markers: a Review

Bioelectrode Applied to Diagnosis of Cardiac Disease

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