An online coupled peritoneal macrophage/cell membrane chromatography and high‐performance liquid chromatography/mass spectrometry method to screen for anti‐inflammatory components from the Chinese traditional medicine <i>Chloranthus multistachys</i> Pei

Liu, Weifeng; Wei, Xing; Wang, Sicen; Fan, Ting; Niu, Xiaofeng; He, Langchong

doi:10.1002/bmc.2963

Cited by 12 publications

(5 citation statements)

References 28 publications

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“…Cells membrane chromatography (CMC), a chromatographic biological affinity method that uses specific cell membranes as the stationary phase, has been confirmed as a simple, specific, effective screening method based on the binding principle that the recognition and interaction of drug molecules and targets, receptors, channels on the cell membrane [ 14 ]. A novel peritoneal macrophage/cell membrane chromatography (PM/CMC)-HPLC/MS method was shown to be an effective screening system for the rapid detection, enrichment, and identification of anti-inflammatory components from TCM [ 14 ]. Herein, we carried out this effective screening method to screen anti-inflammatory components from CB.…”

Section: Discussionmentioning

confidence: 99%

Screening for anti-inflammatory components from Corydalis bungeana Turcz. based on macrophage binding combined with HPLC

Dong

Zhang

Zhao

et al. 2015

BMC Complement Altern Med

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BackgroundCorydalis bungeana Turcz. (CB; family: Corydalis DC.) is an anti-inflammatory medicinal herb used widely in traditional Chinese medicine (TCM) for upper respiratory tract infection, etc., but its anti-inflammatory active molecules are unknown. This study was designed to screen for the anti-inflammatory components from CB based on macrophage binding combined with HPLC.MethodsXylene-induced ear edema in mouse and carrageenan-induced hind-paw edema in rats were used to evaluate the anti-inflammatory activity of CB. The macrophage binding with high-performance liquid chromatography (HPLC) analysis and HPLC-MS were established to screen the potential active compounds. ELISA kits were performed to measure the levels of IL-6, IL-10, TNF-α and NO in RAW 264.7 macrophages culture media.ResultsThe alkaloid extract of CB could inhibit significantly xylene-induced ear edema in mouse and carrageenan-induced hind-paw edema in rats. Two components binded to RAW 264.7 cell were identified as 12-hydroxycorynoline and corynoline. Bioassays demonstrated that these two compounds significantly inhibited LPS-induced IL-6, IL-10, TNF-α and NO levels.ConclusionsThe results suggest that corynoline and 12-hydroxycorynoline contribute to the anti-inflammatory effects of the alkaloid extract of CB. Our findings suggest that these two compounds can be used as candidate for anti-inflammatory drugs.

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Section: Discussionmentioning

confidence: 99%

Screening for anti-inflammatory components from Corydalis bungeana Turcz. based on macrophage binding combined with HPLC

Dong

Zhang

Zhao

et al. 2015

BMC Complement Altern Med

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“…On this basis, increasing research has begun to explore CMC made of different cell membrane target receptors and coupled with GC–MS or HPLC–MS techniques to achieve high throughput screening. CMC of fibroblast growth factor receptor 4 (FGFR4), 80 epidermal growth factor receptor (EGFR), 81 α 1A receptors 82 and peritoneal macrophages (PMs) 83 have been coupled with other chromatographic techniques to screen a wider range of pharmacologically active components.…”

Section: Application Of 2d Chromatography In Screening Of Bioactive C...mentioning

confidence: 99%

Two‐dimensional chromatography in screening of bioactive components from natural products

Xiang

Qiu

et al. 2022

Phytochemical Analysis

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Introduction: Screening and analysis of bioactive components from natural products is a fundamental part of new drug development and innovation. Two-dimensional (2D) chromatography has been demonstrated to be an effective method for screening and preparation of specific bioactive components from complex natural products. Objective: To collect details of application of 2D chromatography in screening of natural product bioactive components and to outline the research progress of different separation mechanisms and strategies. Methodology: Three screening strategies based on 2D chromatography are reviewed, including traditional separation-based screening, bioactivity-guided screening and affinity chromatography-based screening. Meanwhile, in order to cover these aspects, selections of different separation mechanisms and modes are also presented.Results: Compared with traditional one-dimensional (1D) chromatography, 2D chromatography has unique advantages in terms of peak capacity and resolution, and it is more effective for screening and identifying bioactive components of complex natural products. Conclusion:Screening of natural bioactive components using 2D chromatography helps separation and analysis of complex samples with greater targeting and relevance, which is very important for development of innovative drug leads.

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“…Furthermore, mouse leukaemic monocyte macrophage cell line (RAW264.7) was usually used for the development of inflammatory model for studying of anti‐inflammatory drugs . In addition, mouse peritoneal macrophages have been used in bio‐membrane chromatography for screening potential active compounds from complex matrix . However, few studies about the interaction between macrophages and small molecules have been reported.…”

Section: Related Data In Face For Calculating Stoichiometry and Bindimentioning

confidence: 99%

Evaluation of interactions between RAW264.7 macrophages and small molecules by capillary electrophoresis

Wang

Zhang

et al. 2017

Electrophoresis

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In this study, the affinity interactions between RAW 264.7 macrophages and three small molecules including naringin, oleuropein and paeoniflorin were evaluated by affinity capillary electrophoresis (ACE), partial filling affinity capillary electrophoresis (PFACE) and frontal analysis capillary electrophoresis (FACE), respectively. The result indicated that ACE (varying concentrations of cell suspension were filled in the capillary as receptor) may not be suitable for the evaluation of interactions between cell and small molecules due to the high viscosity of cell suspension; PFACE can qualitatively evaluate the interaction, but the difference in viscosity between RAW264.7 suspension and buffer effects on the liner relationship between filling length and injection time, which makes the calculation of binding constant difficult. Furthermore, based on the PFACE results, naringin showed stronger interaction with macrophages than the other two molecules; taking advantage of the aggregation phenomenon of cell induced by electric field, FACE was successfully used to determine the stoichiometry (n = 5×10 ) and binding constant (K = 1×10 L/mol) of the interaction between RAW264.7 and naringin.

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An online coupled peritoneal macrophage/cell membrane chromatography and high‐performance liquid chromatography/mass spectrometry method to screen for anti‐inflammatory components from the Chinese traditional medicine Chloranthus multistachys Pei

Cited by 12 publications

References 28 publications

Screening for anti-inflammatory components from Corydalis bungeana Turcz. based on macrophage binding combined with HPLC

Screening for anti-inflammatory components from Corydalis bungeana Turcz. based on macrophage binding combined with HPLC

Two‐dimensional chromatography in screening of bioactive components from natural products

Evaluation of interactions between RAW264.7 macrophages and small molecules by capillary electrophoresis

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