An Open Tubular CEC Column of Excellent Separation Efficiency for Proteomic Analysis

Cheong, Won Jo; Zaidi, Shabi Abbas; Kim, Yune Sung

doi:10.5012/bkcs.2014.35.10.3115

Cited by 11 publications

(18 citation statements)

References 39 publications

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“…The difference in the electrophoretic mobilities of proteins and PNE coating combined to play roles for the separation of these proteins. In comparison with the previous work aiming at the separation of proteins by OT‐CEC , the PNE‐coated column showed good separation ability for acidic and basic proteins. More importantly, the column can resolve the glycoforms of rhEPO.…”

Section: Discussionmentioning

confidence: 69%

Poly(norepinephrine)‐coated open tubular column for the separation of proteins and recombination human erythropoietin by capillary electrochromatography

Xue

Zhang

et al. 2017

J of Separation Science

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Recombinant human erythropoietin is an important therapeutic protein with high economic interest due to the benefits provided by its clinical use for the treatment of anemias associated with chronic renal failure and chemotherapy. In this work, a poly(norepinephrine)‐coated open tubular column was successfully prepared based on the self‐polymerization of norepinephrine under mild alkaline condition, the favorable film forming and easy adhesive properties of poly(norepinephrine). The poly(norepinephrine) coating was characterized by scanning electron microscopy and measurement of the electro‐osmotic flow. The thickness of the coating was about 431 nm. The electrochromatographic performance of the poly(norepinephrine)‐coated open tubular column was evaluated by separation of proteins. Some basic and acidic proteins including two variants of bovine serum albumin and two variants of β‐lactoglobulin achieved separation in the poly(norepinephrine)‐coated open tubular column. More importantly, the column demonstrated separation ability for the glycoforms of recombinant human erythropoietin. In addition, the column demonstrated good repeatability with the run‐to‐run, day‐to‐day, and column‐to‐column relative standard deviations of migration times of proteins less than 3.40%.

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Section: Discussionmentioning

confidence: 69%

Poly(norepinephrine)‐coated open tubular column for the separation of proteins and recombination human erythropoietin by capillary electrochromatography

Xue

Zhang

et al. 2017

J of Separation Science

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“…De Smet and Lynen produced via a single step in situ polymerization process a porous poly(styrene‐divinylbenzene‐vinylsulfonic acid) layer, introduced to a 3‐(trimethoxysilyl) propylmethacrylate‐modified wall, achieving a layer thickness of 400–600 nm. Cheong and coworkers prepared MIP columns by in situ polymerization of 4‐aminostyrene, ethylene glycol dimethacrylate, AIBN in ACN/2‐propanol using PEG (MW 10 000) as the template molecule . PEG was selected as template molecule for compatibility with solvent system of reaction mixture and was used for the formation of pores.…”

Section: Preparation Approaches For Ot‐cec Coatingsmentioning

confidence: 99%

Open tubular‐capillary electrochromatography: Developments and applications from 2013 to 2015

et al. 2016

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Open tubular CEC (OT-CEC) separates analyte mixtures by a combination of electrophoretic, electro-osmotic, and/or chromatographic effects. OT-CEC research is an active and growing field, with studies encompassing a wide range of investigations related to new strategies for chemical modification of the inner surface of the capillary, leading to the introduction of novel stationary phase coatings. This review has examined the literature on OT-CEC from 2013 to August 2015 and highlights the developments in the fabrication of highly selective stationary phases, based on materials that include cyclodextrin chiral selectors, graphene and graphene oxide, metal-organic frameworks, molecularly imprinted polymers, nanoparticles, nanolatex particles, nanocomposites, in situ generated polymers, block polymers, tentacle-type polymers, polyelectrolyte multilayers, polysaccharides, phospholipids, and proteins. This review, while considering the development of novel OT-CEC coating materials, specifically examines different immobilization or coating methodologies and approaches and also discusses the separation mechanisms that occur with these new materials. These OT-CEC coatings are intended mainly to separate low molecular weight molecules relevant to the pharmaceutical, agricultural, and food industries as well as for use in environmental monitoring.

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“…The OT-CEC column of this study shows comparable performance. An OT-CEC column has been recently prepared in our laboratory to show very high separation efficiency (more than 200 000 plates/m) in separation of cytochrome C tryptic digest where the MIP technique was incorporated in formation of an organic monolith layer on the inner wall of the silica capillary [34]. The separation performances of some of the above studies and the current study are collectively compared in Fig.…”

Section: Comparison Of Separation Performance With Those Of Previous mentioning

confidence: 99%

“…Pretreatment of the fused-silica capillary (50 cm length, 360 m od, and 50 m id) was carried out according to the reported procedure [33,34]. Briefly, 0.1 M NaOH solution was filled in the capillary, end-capped with septa and kept for 24 h at room temperature, then at 55ЊC overnight.…”

Section: Pretreatmentmentioning

confidence: 99%

“…CEC columns can also be employed in multidimensional separation [33]. Our research group has reported the application of molecularly imprinted polymer (MIP) incorporated OT-CEC column for proteomic analysis of cytochrome C tryptic digest [34] with excellent separation efficiency, but the peptide peaks were assembled in a narrow range of retention time and the peak capacity was rather limited in that study.…”

Section: Introductionmentioning

confidence: 99%

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Open tubular capillary column for the separation of cytochrome C tryptic digest in capillary electrochromatography

Ali

Cheong

2015

J of Separation Science

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A silica capillary of 50 μm internal diameter and 500 mm length (416 mm effective length) was chemically modified with 4-(trifluoromethoxy) phenyl isocyanate in the presence of dibutyl tin dichloride as catalyst. Sodium diethyl dithiocarbamate was reacted with the terminal halogen of the bound ligand to incorporate the initiator moiety, and in situ polymerization was performed using a monomer mixture of styrene, N-phenylacrylamide, and methacrylic acid. The resultant open tubular capillary column immobilized with the copolymer layer was used for the separation of tryptic digest of cytochrome C in capillary electrochromatography. The sample was well eluted and separated into many components. The elution patterns of tryptic digest of cytochrome C were studied with respect to pH and water content in the mobile phase. This preliminary study demonstrates that open tubular capillary electrochromatography columns with a modified copolymer layer composed of proper nonpolar and polar units fabricated by reversible addition-fragmentation transfer polymerization can be useful as separation media for proteomic analysis.

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An Open Tubular CEC Column of Excellent Separation Efficiency for Proteomic Analysis

Cited by 11 publications

References 39 publications

Poly(norepinephrine)‐coated open tubular column for the separation of proteins and recombination human erythropoietin by capillary electrochromatography

Poly(norepinephrine)‐coated open tubular column for the separation of proteins and recombination human erythropoietin by capillary electrochromatography

Open tubular‐capillary electrochromatography: Developments and applications from 2013 to 2015

Open tubular capillary column for the separation of cytochrome C tryptic digest in capillary electrochromatography

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