An optical method to detect tissue scattering: theory, experiments and biomedical applications

Yariv, Inbar; Duadi, Hamootal; Fixler, Dror

doi:10.1117/12.2508335

Cited by 7 publications

(28 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reconstructed phase image shows two distinguishable areas, separated by a circular border, which was reported in our previous papers [21][22][23][24]. The cyan circle, in the center of the phase image, represents the area closer to the radiation source, which is the single scattering regime.…”

Section: Resultssupporting

confidence: 67%

“…The IMOPE technique is built on three fundamental segments: a theoretical model (described in detail in [24]), an experimental setup and the GS algorithm [26]. The theoretical model constructs the connection between the reduced scattering coefficient (s') of a medium and its reemitted light phase distribution.…”

Section: Iterative Multi-plane Optical Properties Extraction (Imope)mentioning

confidence: 99%

“…We have presented previously the iterative multi-plane optical property extraction (IMOPE) technique [19][20][21][22][23][24][25]. The IMOPE technique is a noninvasive nanophotonics technique for extracting the scattering properties of a medium based on a single light source.…”

Section: Introductionmentioning

confidence: 99%

“…The IMOPE was initially applied in a transmission mode where its ability to detect organic nanoparticles within tissues [19] and the quantitative signature of milk components (lactose and milk protein) [20] was reported. Next, the reflection-based IMOPE was constructed, where its theoretical model [24] was This work is licensed under a Creative Commons Attribution 4.0 License. For more information, see https://creativecommons.org/licenses/by/4.0/.…”

Section: Introductionmentioning

confidence: 99%

“…First, single layer tissue-like phantoms were measured by the IMOPE, for calibration purposes. As was reported before [22][23][24][25], two distinguishable regions were visible in the phase images and were marked manually as two spatial regions that correlated to two scattering regimes. Here, a methodology for finding these two regions automatically based on RMS computations from the phase images is presented.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Depth Scattering Characterization of Multi-Layer Turbid Media Based on Iterative Multi-Plane Reflectance Measurements

2020

Self Cite

View full text Add to dashboard Cite

Overcoming opaque barriers beyond the penetration depth is based on light-matter interaction, with applications such as biomedical optics, material science and security. In wave propagation, scattering is the amount of disorder, mostly treated as a disturbance. Scattered light variations allow overcoming opaque barriers. In order to separate turbid medium layers, absorption-based contrast is commonly used. This paper presents the two-layer study of a noninvasive scattering-based technique. The iterative multiplane optical property extraction (IMOPE) technique assesses scattering by reconstructing the light phase. The reflection-based IMOPE detected different scattering layers with 0.2mm-1 sensitivity at different depths, behind layers up to 6mm thick.

show abstract

Section: Resultssupporting

confidence: 67%

Section: Iterative Multi-plane Optical Properties Extraction (Imope)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Depth Scattering Characterization of Multi-Layer Turbid Media Based on Iterative Multi-Plane Reflectance Measurements

2020

Self Cite

View full text Add to dashboard Cite

show abstract

Noninvasive Nanodiamond Skin Permeation Profiling Using a Phase Analysis Method: Ex Vivo Experiments

et al. 2022

View full text Add to dashboard Cite

Carbon-based nanoparticles (NPs) are widely used in nanotechnology. Among them, nanodiamonds (NDs) are suitable for biotechnology and are especially interesting for skin delivery and topical treatments. However, noninvasive detection of NDs within the different skin layers or analyzing their penetration ability is complicated due to the turbid nature of the tissue. The iterative multiplane optical properties extraction (IMOPE) technique detects differences in the optical properties of the measured item by a phase-image analysis method. The phase image is reconstructed by the multiplane Gerchberg–Saxton algorithm. This technique, traditionally, detects differences in the reduced scattering coefficients. Here, however, due to the actual size of the NDs, the IMOPE technique’s detection relies on absorption analysis rather than relying on scattering events. In this paper, we use the IMOPE technique to detect the presence of the NDs within tissue-like phantoms. In addition, we perform ex vivo pigskin experiments to estimate the penetration of the NDs to the different skin layers and show that their presence reduces at deeper layers. The significance signal of the NDs within the epidermis, dermis, and fat layers gradually reduces, with t test significance values that are smaller than 10–4, 10–3, and 10–2, respectively. The IMOPE results are corroborated by TEM results and Franz-cell experiments. These results confirm that the IMOPE profiled the skin-permeation of the NDs noninvasively.

show abstract

Media Characterization under Scattering Conditions by Nanophotonics Iterative Multiplane Spectroscopy Measurements

et al. 2019

Self Cite

View full text Add to dashboard Cite

Characterizing materials is preferably done by multiple wavelengths. In opaque materials, the scattering poses a challenge due to the additional complexity to the spectroscopic measurements. We have previously demonstrated an iterative multiplane method for characterizing materials using the reflection from turbid media. Initial studies were performed in the red wavelength regime (632.8 nm) which is optimal for biomedical applications. However, in order to differentiate between materials, it is better to use multiple wavelengths, as spectroscopy may detect the material fingerprint. In this paper, our iterative multiplane optical property extraction (IMOPE) technique is presented in the blue regime (473 nm). Agar-based solid phantom measurements were conducted and compared to our theoretical model. Compatibility between experiments in the red and blue wavelengths shows the robustness of our technique.

show abstract

An optical method to detect tissue scattering: theory, experiments and biomedical applications

Cited by 7 publications

References 40 publications

Depth Scattering Characterization of Multi-Layer Turbid Media Based on Iterative Multi-Plane Reflectance Measurements

Depth Scattering Characterization of Multi-Layer Turbid Media Based on Iterative Multi-Plane Reflectance Measurements

Noninvasive Nanodiamond Skin Permeation Profiling Using a Phase Analysis Method: Ex Vivo Experiments

Media Characterization under Scattering Conditions by Nanophotonics Iterative Multiplane Spectroscopy Measurements

Contact Info

Product

Resources

About