An optimized method for Oil Red O staining with the salicylic acid ethanol solution

Abstract

Oil Red O (ORO) staining is a commonly used experimental technique to detect lipid content in cells or tissues. Freshly prepared ORO in 60% isopropanol is the most widely used method at present. However, isopropanol is volatile and harmful to the human body. It will also affect the interpretation of the results due to the formation of crystals and non-specific diffuse staining. In this paper, by screening and validation, we report a salicylic acid ethanol solution (containing 50% ethanol, 5%-10% salicylic acid) for the preparation of ORO solution, which has a better staining effect on lipid staining in cells and tissues, with a clean background and short dyeing time. What’s more, this ORO solution is non-toxic, convenient to prepare, and can be stored for a long time. Therefore, it is reliable, easy to operate, and can be widely popularized and applied in laboratories.

Distinct mechanisms of non-autonomous UPRERmediated by GABAergic, glutamatergic, and octopaminergic neurons

“…4B ). To further evaluate changes in lipid content, we utilized a more comprehensive dye, Oil Red O (ORO), which stains neutral lipids, cholesteryl esters, and lipoproteins 36 . Consistent with lipid droplet imaging, we observed a significant decrease in lipid content in glutamatergic, octopaminergic, or GABAergic xbp-1s animals using ORO ( Fig.…”

Response surface methodology optimization of extraction and enrichment conditions of total triterpenoid saponins from Celosiae Semen and evaluation of its lipid‐lowering activity

“…Cell viability was the percentage absorbance value of the treated cell relative to the untreated cell. Oil Red O, a fat-soluble dye that is usually used to stain neutral lipids, cholesterol esters and lipoproteins, has been long used for intracellular lipid staining and for tissue staining owing to its cellular permeability (Bharati et al, 2022;Du et al, 2023). Neutral lipids (mainly triglycerides) are stained with an orange-red tint, based on the higher solubility of Oil Red O in lipids compared with the dye solvents (Koopman et al, 2001).…”

ROS-responsive curcumin-encapsulated nanoparticles for AKI therapy via promoting lipid degradation in renal tubules

“…Oil Red O is a fat-soluble dye that selectively binds neutral triglycerides and lipids, allowing visualization of intracellular lipid droplets. 52,53 Significant cytoplasmic Oil Red O staining was observed in proximal tubular cells of AKI mouse kidneys, indicative of ectopic lipid accumulation and cellular damage. However, treatment with Cur and NPS BG @Cur significantly led to a notable reduction in Oil Red O staining intensity and lipid droplet deposition compared to AKI group kidneys.…”