An optimized visual loop mediated isothermal amplification assay for efficient detection of minute virus of mice with hydroxynaphthol blue dye

Zhang, Xuliang; Zhao, Yingfeng; Ma, Chang; Wu, Wei; Dong, Min; You, Jinwei; Liu, Jie; Yun, Shifeng

doi:10.1016/j.jviromet.2022.114575

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…As the concentration of Mg 2+ in the solution decreases during the LAMP process, the color of the HNB solution changes from purple to blue [ 20 ]. Furthermore, HNB does not affect the LAMP reaction [ 57 ]; hence, it is widely used as a reliable indicator [ 60 , 61 , 62 , 63 , 64 ].…”

Section: Monitoring Methods For Lampmentioning

confidence: 99%

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Principles and Applications of Loop-Mediated Isothermal Amplification to Point-of-Care Tests

Park

2022

Biosensors

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For the identification of nucleic acids, which are important biomarkers of pathogen-mediated diseases and viruses, the gold standard for NA-based diagnostic applications is polymerase chain reaction (PCR). However, the requirements of PCR limit its application as a rapid point-of-care diagnostic technique. To address the challenges associated with regular PCR, many isothermal amplification methods have been developed to accurately detect NAs. Isothermal amplification methods enable NA amplification without changes in temperature with simple devices, as well as faster amplification times compared with regular PCR. Of the isothermal amplifications, loop-mediated isothermal amplification (LAMP) is the most studied because it amplifies NAs rapidly and specifically. This review describes the principles of LAMP, the methods used to monitor the process of LAMP, and examples of biosensors that detect the amplicons of LAMP. In addition, current trends in the application of LAMP to smartphones and self-diagnosis systems for point-of-care tests are also discussed.

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Section: Monitoring Methods For Lampmentioning

confidence: 99%

“…Using HNB, 10 4 copies of minute viruses of mice were detected; the value was 100-fold lower than that of the conventional PCR [ 64 ]. In another case, using HNB-based LAMP, 0.04 pg of Chlamydia trachomatis was detected with 95% specificity and 90–100% sensitivity [ 61 ].…”

Section: Monitoring Methods For Lampmentioning

confidence: 99%