2016
DOI: 10.1523/jneurosci.3482-15.2016
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An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons

Abstract: To investigate excitatory and inhibitory GABA actions in cortical neuronal networks, we present a novel optogenetic approach using a mouse knock-in line with conditional expression of channelrhodopsin-2 (ChR2) in GABAergic interneurons. During whole-cell recordings from hippocampal and neocortical slices from postnatal day (P) 2-P15 mice, photostimulation caused depolarization and excitation of interneurons and evoked barrages of postsynaptic GABAergic currents. Excitatory/inhibitory GABA actions on pyramidal … Show more

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Cited by 112 publications
(132 citation statements)
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“…Therefore, our modeling results (Fig. 4b) are in agreement with recent in vivo findings that while GABA acts as a mainly depolarizing transmitter during the first postnatal week, it exerts an inhibitory effect on the underlying network activity in the immature cortex and hippocampus 20,31 .…”
Section: Resultssupporting
confidence: 92%
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“…Therefore, our modeling results (Fig. 4b) are in agreement with recent in vivo findings that while GABA acts as a mainly depolarizing transmitter during the first postnatal week, it exerts an inhibitory effect on the underlying network activity in the immature cortex and hippocampus 20,31 .…”
Section: Resultssupporting
confidence: 92%
“…This simulated manipulation led to an increase in the cluster activity size only when GABAergic transmission was inhibitory at the network level. Therefore, our results support the recent in vivo findings 31,33 . Importantly, this finding implies that even when GABA depolarizes immature neurons, GABA still can attenuate the instability effect on network activity, thereby restricting cluster activity size.…”
Section: Discussionsupporting
confidence: 93%
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“…What are the consequences for the generation of early network activity? In order to address the question, Valeeva and colleagues recently employed an optogenetic strategy by selectively expressing channelrhodopsin-2 in GABAergic interneurons and recording glutamatergic PSCs (EPSCs) (Valeeva et al, 2016). The latter serve as a measure of activity of presynaptic glutamatergic neurons.…”
Section: Cellular and Network Effects Of Gabaergic Transmission In Thmentioning
confidence: 99%