An organoid model derived from human adipose stem/progenitor cells to study adipose tissue physiology

Mandl, Markus; Viertler, Hans P.; Hatzmann, Florian M.; Brucker, Camille; Großmann, Sonja; Waldegger, Petra; Rauchenwald, Tina; Mattesich, Monika; Zwierzina, Marit; Pierer, Gerhard; Zwerschke, Werner

doi:10.1080/21623945.2022.2044601

Cited by 21 publications

(17 citation statements)

References 44 publications

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“…134 Additionally, it is possible to recognize and study the physiological and histological problems in people with obesity without using human adipose tissue, which is obtained through autopsy and surgical operations. 135 In the future, we believe that this will serve as the catalyst to broaden the combined application of in vitro patient-derived iPSC models and in vivo rodent models to aid in our understanding of the various facets of obesity and other metabolic diseases. 136 New insights are continuously offered to us by technological breakthroughs.…”

Section: Hpsc-derived Models Of Adipose Organoidsmentioning

confidence: 99%

Epigenetics in obesity: Mechanisms and advances in therapies based on natural products

Chen,

Wang,

Chen

et al. 2024

Pharmacology Res & Perspec

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Obesity is a major risk factor for morbidity and mortality because it has a close relationship to metabolic illnesses, such as diabetes, cardiovascular diseases, and some types of cancer. With no drugs available, the mainstay of obesity management remains lifestyle changes with exercise and dietary modifications. In light of the tremendous disease burden and unmet therapeutics, fresh perspectives on pathophysiology and drug discovery are needed. The development of epigenetics provides a compelling justification for how environmental, lifestyle, and other risk factors contribute to the pathogenesis of obesity. Furthermore, epigenetic dysregulations can be restored, and it has been reported that certain natural products obtained from plants, such as tea polyphenols, ellagic acid, urolithins, curcumin, genistein, isothiocyanates, and citrus isoflavonoids, were shown to inhibit weight gain. These substances have great antioxidant potential and are of great interest because they can also modify epigenetic mechanisms. Therefore, understanding epigenetic modifications to target the primary cause of obesity and the epigenetic mechanisms of anti‐obesity effects with certain phytochemicals can prove rational strategies to prevent the disease and develop novel therapeutic interventions. Thus, the current review aimed to summarize the epigenetic mechanisms and advances in therapies for obesity based on natural products to provide evidence for the development of several potential anti‐obesity drug targets.

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Section: Hpsc-derived Models Of Adipose Organoidsmentioning

confidence: 99%

Epigenetics in obesity: Mechanisms and advances in therapies based on natural products

Chen,

Wang,

Chen

et al. 2024

Pharmacology Res & Perspec

View full text Add to dashboard Cite

show abstract

“…Both models can contribute to the study of obesity, including the endocrine disruptor effect described for many compounds (reviewed by [ 99 ]). As there is always room for improvement, recently developed adipogenesis models can take anti-obesity research even further, such as the case of 3D organoids derived from human adipose stem cells for adipose tissue physiology studies [ 100 ].…”

Section: Alternative Methods To Animal Testing In Adipogenesismentioning

confidence: 99%

Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches

Pamplona

Zoehler²,

Shigunov³

et al. 2022

Life

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The study of adipogenesis is essential for understanding and treating obesity, a multifactorial problem related to body fat accumulation that leads to several life-threatening diseases, becoming one of the most critical public health problems worldwide. In this review, we propose to provide the highlights of the adipogenesis study based on in vitro differentiation of human mesenchymal stem cells (hMSCs). We list in silico methods, such as molecular docking for identification of molecular targets, and in vitro approaches, from 2D, more straightforward and applied for screening large libraries of substances, to more representative physiological models, such as 3D and bioprinting models. We also describe the development of physiological models based on microfluidic systems applied to investigate adipogenesis in vitro. We intend to identify the main alternative models for adipogenesis evaluation, contributing to the direction of preclinical research in obesity. Future directions indicate the association of in silico and in vitro techniques to bring a clear picture of alternative methods based on adipogenesis as a tool for obesity research.

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“…Three-dimensional adipose cell cultures in spheroids and other types of support matrix offer the potential to develop an organoid that better represents the intrinsic three-dimensional structure and function of adipose tissue, with vascularization, immune cells and progenitors. Several systems have been described that offer the opportunity for investigating more complex physiology in vitro, although these systems do not yet fully recapitulate adipose tissue and indeed are comprised of single cell types or mixtures of cells from primary tissue isolation [43][44][45][46]. These three-dimensional models show improved differentiation with larger lipid droplets and may offer high throughput applications with better modelling of tissue.…”

Section: Modelling Adipose Tissuementioning

confidence: 99%

The genetics of adipose tissue metabolism

Dumbell,

Cox

2024

R. Soc. Open Sci.

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An organoid model derived from human adipose stem/progenitor cells to study adipose tissue physiology

Cited by 21 publications

References 44 publications

Epigenetics in obesity: Mechanisms and advances in therapies based on natural products

Epigenetics in obesity: Mechanisms and advances in therapies based on natural products

Alternative Methods as Tools for Obesity Research: In Vitro and In Silico Approaches

The genetics of adipose tissue metabolism

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