An Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae) Baculovirus Inoculum Derived from Tissue Culture

INFECTIVITY OF Oryctes NUDIVIRUS PRODUCED ON CELL CULTURE DSIR HA-1179 AGAINST LARVAE AND ITS EFFECTS ON FEEDING OF NEONATES OF RHINOCEROS BEETLE, Oryctes rhinoceros

“…As this technology enables the production of OrNV with high purity, it has been widely used by various researchers to release the OrNV to control rhinoceros beetles in both coconut and oil palm plantations (Crawford and Sheehan, 1984;Zelazny et al, 1987). Our previous study showed that the Malaysian OrNV types A, B, and C have been successfully produced on insect cell line DSIR-HA-1179 and all OrNV inocula were proven highly pathogenic on adults rhinoceros beetle.…”

Complete Genome Sequences of Six Isolates of the Oryctes rhinoceros Nudivirus

“…OrNV isolates ( Table 1 ) were cultured and maintained in adherent cells of the Heteronychus arator DSIR-HA-1179 cell line ( 3 ) as previously described ( 2 , 4 ). Infected cells were confirmed by microscopy, detached, collected by centrifugation, resuspended in water, and lysed by sonication, using 5 rounds of 20 s (MSE Sanyo Soniprep 150 sonicator; amplitude, 6.5 μm; 23 KHz).…”

Oryctes virus—Time for a new look at a useful biocontrol agent

“…Any active virus distribution system will need to provide reliable inoculum. CertiWed inoculum can be prepared by sterile Wltration (Zelazny et al, 1987) or from infected scarab cell cultures (Crawford and Sheehan, 1984) but stable formulations are required for distribution through the tropics.…”