An Overview of Analytical Techniques Employed to Evidence Drug-DNA Interactions. Applications to the Design of Genosensors

González‐Ruiz, Víctor; Olives, Ana I.; Martín, M. Antonia; Ribelles, Pascual; Ramos, M. Teresa; Menéndez, J. Carlos

doi:10.5772/13586

Cited by 36 publications

(28 citation statements)

References 58 publications

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“…Ghosh et al decrease in fluorescence intensity was observed on addition of small quantities of both types of DNA. This decrease in fluorescence intensity is indicative of intercalation, where the drug molecules are inserted into the base stack of the DNA helix favoring radiationless deactivation of the excited states (Gonzalez-Ruiz et al, 2011). The quenching effect shows a good adjustment to the Stern-Volmer equation.…”

mentioning

confidence: 96%

Biophysical studies of mutated K562 DNA (erythroleukemic cells) binding to adriamycin and daunomycin reveal that mutations induce structural changes influencing binding behavior

Ghosh

Saha

Hossain

et al. 2013

Journal of Biomolecular Structure and Dynamics

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K562 cells are erythroleukemic cells derived from a chronic myeloid leukemia patient in blast crisis. Comparison of the genome from K562 cells and normal human genome has been very useful strategy, in uncovering eight genes, implicated in acute myeloid leukemia (AML). These genes carry mutations in K562 genome and the role of these mutations in the progression and treatment of AML is still not known. Consequences of these mutations on drug DNA binding are also not known exactly. In the present study, mutation induced structural changes in K562 genome, compared to normal genome, are identified by Fourier transform infra red (FTIR) and circular dichroism (CD) spectroscopy. These structural changes in native K562 DNA favor stronger binding with binding constants 2.0 × 10⁸ and 1.9 × 10⁹ M⁻¹ with antileukemic drugs adriamycin and daunomycin (DNM), respectively, compared to normal DNA. On binding, these drugs disrupt the native B form structure of normal DNA to a greater extent, compared to A-like structure of K562 DNA. Fluorescence and absorption studies reveal higher intercalation as well as mixed groove binding of these drugs with K562 DNA compared to normal DNA. Among the drugs, DNM has higher affinity for K562 DNA.

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mentioning

confidence: 96%

Biophysical studies of mutated K562 DNA (erythroleukemic cells) binding to adriamycin and daunomycin reveal that mutations induce structural changes influencing binding behavior

Ghosh

Saha

Hossain

et al. 2013

Journal of Biomolecular Structure and Dynamics

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“…Other recent literatures also reviewed that the study of DNA interaction with a small molecules is of high interest [7,[29][30][31][32]. Thus, the present theoretical analysis can be applied to understand bimolecular interaction and may also be implicated to the process of drug development in biomedical industries.…”

Section: Discussionmentioning

confidence: 84%

Drug-DNA Interaction: A Theoretical Study of the Stability of CP-DNA Binding with Thionine

Yunus¹,

Srivastava²,

Gupta³

2012

OJAppS

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The recent study on binding of small molecules to double stranded DNA suggested that the intercalation of a tricyclic heteroaromatic molecule, thionine, with natural DNA provided thermal stabilization to the complex. In the present study, we reported theoretical analysis of thionine binding with Clostridium perfringenes DNA duplex (CP-DNA) by using an amended Zimm and Bragg theory, to explain the melting behaviour and heat capacity of CP-DNA with and without thionine binding. The experimental models of Paul et al. (2010) have been used for the study. The sharpness of transition has been examined in terms of half width and sensitivity parameter (∆H/σ). The results of theoretical approach suggested that the various parameters such as transition profile, sharpness of the transition, heat capacity curve and half widths are in good agreement with the experimental measurements for binding of thionine. Therefore, the proposed theoretical analysis may be useful in order to understand interaction of small molecules to DNA that may be applied in the process of drug development and for designing more potential DNA binding therapeutic molecules.

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“…Sundaram et al (2012) reported that 1000-1150 and 1170-1280 cm À1 are important for Salmonella serotype classification. These spectral regions were sensitive to the effects of base pairing, base stacking and glycosidic bond rotation between the backbone and sugar pucker (Brewer et al 2002;Gonz alez-Ruiz et al 2011). Based on these results, we predicted that characteristic FT-IR spectral differences were caused by the inherent nature of genomic DNA, rather than the impurity of genomic DNA.…”

Section: Discussionmentioning

confidence: 92%

“…; González‐Ruiz et al . ). Based on these results, we predicted that characteristic FT‐IR spectral differences were caused by the inherent nature of genomic DNA, rather than the impurity of genomic DNA.…”

Section: Discussionmentioning

confidence: 97%

Rapid tool for identification of bacterial strains using Fourier transform infrared spectroscopy on genomic DNA

et al. 2019

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Aims We developed a new rapid and reliable method for identifying bacteria using a combination of Fourier transform infrared (FT‐IR) spectroscopy of bacterial genomic DNA and multivariate analysis. Methods and Results FT‐IR spectra of genomic DNA from four type strains of Pseudomonas spp., three type strains of Escherichia spp. and two type strains of Bacillus spp. were analysed in the 4000–400 cm−1 region. Spectral differences were found in the frequency regions of N–H stretching (amide I), C=O stretching vibrations (amide II) and PO2− ionized asymmetric and symmetric stretching. Partial least squares discriminant analysis of the FT‐IR spectra showed that the microbial strains could be discriminated by hierarchical clustering analysis. Conclusions FT‐IR spectral analysis of bacterial genomic DNA has potential for the rapid identification of bacteria at the genus and species levels. Significance and Impact of the Study This study reports a new bacterial identification method using multivariate analysis of FT‐IR spectra of bacterial genomic DNA.

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An Overview of Analytical Techniques Employed to Evidence Drug-DNA Interactions. Applications to the Design of Genosensors

Cited by 36 publications

References 58 publications

Biophysical studies of mutated K562 DNA (erythroleukemic cells) binding to adriamycin and daunomycin reveal that mutations induce structural changes influencing binding behavior

Biophysical studies of mutated K562 DNA (erythroleukemic cells) binding to adriamycin and daunomycin reveal that mutations induce structural changes influencing binding behavior

Drug-DNA Interaction: A Theoretical Study of the Stability of CP-DNA Binding with Thionine

Rapid tool for identification of bacterial strains using Fourier transform infrared spectroscopy on genomic DNA

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