An Overview of HLA Typing for Hematopoietic Stem Cell Transplantation

Little, Ann‐Margaret

doi:10.1007/978-1-59745-223-6_3

“…HLA genes are highly polymorphic and have a significant impact on the outcome of organ transplant (SOT), hematopoietic stem cell transplantation (HSCT), and pharmacogenetics. 1–6 In HSCT, serious complications such as graft rejection and graft-versus-host disease could be minimized by matching HLA alleles of donor and recipient prior to transplant. 7 In the case of pharmacogenomics, several drugs such as abacavir and carbamazepine may cause diverse reactions, as these drugs appear to react with the antigen-binding groove of certain HLA alleles causing T-cell mediated hypersensitivity.…”

Section: Introductionmentioning

confidence: 99%

HLA-A, -B, -C, -DRB1, and -DQB1 Allele Lineages and Haplotype Frequencies among Saudis

Osman

¹

,

Gonzalez-Galarza

²

,

Mubasher

³

et al. 2014

Immunol Immunogenet Insights

3

2

0

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ABSTRACT:There are few reported studies on Saudi population for human leukocyte antigens (HLA) genes. We investigated allele lineages (two-digit) and haplotype frequencies of HLA-A, -B, -C, -DRB1, and -DQB1 loci in 499 healthy unrelated individuals, selected from potential bone marrow transplant (BMT) families' donors at King Fahad Medical City (KFMC), Saudi Arabia (SA). Genotyping was performed by Sequence Specific Oligonucleotide Probe (SSOP) utilizing a Luminex-based method. Allele lineages and haplotype frequencies were evaluated along with principal component analysis (PCA) to compare findings with previously reported data on Arab related populations. A total of 18 allele lineages for HLA-A, 28 for -B, 14 for -C, 13 for -DRB1, and 5 for -DQB1 were detected. High values for linkage disequilibrium indicators were found for B:C (D′ = 0.86599) and DRB1:DQB1 (D′ = 0.89468) loci. Additionally, PCA results confirmed previous findings on this population, but also indicated some genetic distances from other Arab related populations. The present study helps in further investigations of this population in anthropological analysis and HLA-associated disease studies.KEY WORDS: HLA, allele, frequencies, Saudis CITATION: osman et al. hla-a, -B, -c, -dRB1, and -dQB1 allele lineages and haplotype Frequencies among saudis.

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“…HLA genes are highly polymorphic and have a significant impact on the outcome of organ transplant (SOT), hematopoietic stem cell transplantation (HSCT), and pharmacogenetics. [1][2][3][4][5][6] In HSCT, serious complications such as graft rejection and graft-versus-host disease could be minimized by matching HLA alleles of donor and recipient prior to transplant. 7 In the case of pharmacogenomics, several drugs such as abacavir and carbamazepine may cause diverse reactions, as these drugs appear to react with the antigen-binding groove of certain HLA alleles causing T-cell mediated hypersensitivity.…”

Section: Introductionmentioning

confidence: 99%

HLA-A, -B, -C, -DRB1, and -DQB1 Allele Lineages and Haplotype Frequencies among Saudis

Osman

¹

,

Gonzalez-Galarza

²

,

Mubasher

³

et al. 2014

III

View full text Add to dashboard Cite

ABSTRACT:There are few reported studies on Saudi population for human leukocyte antigens (HLA) genes. We investigated allele lineages (two-digit) and haplotype frequencies of HLA-A, -B, -C, -DRB1, and -DQB1 loci in 499 healthy unrelated individuals, selected from potential bone marrow transplant (BMT) families' donors at King Fahad Medical City (KFMC), Saudi Arabia (SA). Genotyping was performed by Sequence Specific Oligonucleotide Probe (SSOP) utilizing a Luminex-based method. Allele lineages and haplotype frequencies were evaluated along with principal component analysis (PCA) to compare findings with previously reported data on Arab related populations. A total of 18 allele lineages for HLA-A, 28 for -B, 14 for -C, 13 for -DRB1, and 5 for -DQB1 were detected. High values for linkage disequilibrium indicators were found for B:C (D′ = 0.86599) and DRB1:DQB1 (D′ = 0.89468) loci. Additionally, PCA results confirmed previous findings on this population, but also indicated some genetic distances from other Arab related populations. The present study helps in further investigations of this population in anthropological analysis and HLA-associated disease studies.KEY WORDS: HLA, allele, frequencies, Saudis CITATION: osman et al. hla-a, -B, -c, -dRB1, and -dQB1 allele lineages and haplotype Frequencies among saudis.

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