An Overview of <i>In Vitro</i>, <i>In Vivo</i>, and Computational Techniques for Cancer‐Associated Angiogenesis Studies

Rahman, Heshu Sulaiman; Tan, Bee Ling; Othman, Hemn Hassan; Chartrand, Max Stanley; Pathak, Yashwant; Mohan, Syam; Abdullah, Rasedee; Alitheen, Noorjahan Banu

doi:10.1155/2020/8857428

Cited by 9 publications

(7 citation statements)

References 105 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All of these techniques are constrained by the necessity for surgical resection and result in a restricted distribution area; hence, they cannot control the full range of infiltrative BT. Furthermore, the regulatory process for such devices is time-consuming, necessitating a fresh testing and approval process for each combination of delivery method and medicine [ 95 ]. Local delivery systems, however, provide a direct, well-tolerated, and effective therapy in certain individuals and may serve as the cornerstone of a successful multimodal strategy.…”

Section: Possible Route Of Administrationmentioning

confidence: 99%

Involvement of Resveratrol against Brain Cancer: A Combination Strategy with a Pharmaceutical Approach

et al. 2022

View full text Add to dashboard Cite

A brain tumor (BT) is a condition in which there is growth or uncontrolled development of the brain cells, which usually goes unrecognized or is diagnosed at the later stages. Since the mechanism behind BT is not clear, and the various physiological conditions are difficult to diagnose, the success rate of BT is not very high. This is the central issue faced during drug development and clinical trials with almost all types of neurodegenerative disorders. In the first part of this review, we focus on the concept of brain tumors, their barriers, and the types of delivery possible to target the brain cells. Although various treatment methods are available, they all have side effects or toxic effects. Hence, in the second part, a correlation was made between the use of resveratrol, a potent antioxidant, and its advantages for brain diseases. The relationship between brain disease and the blood–brain barrier, multi-drug resistance, and the use of nanomedicine for treating brain disorders is also mentioned. In short, a hypothetical concept is given with a background investigation into the use of combination therapy with resveratrol as an active ingredient, the possible drug delivery, and its formulation-based approach.

show abstract

Section: Possible Route Of Administrationmentioning

confidence: 99%

Involvement of Resveratrol against Brain Cancer: A Combination Strategy with a Pharmaceutical Approach

et al. 2022

View full text Add to dashboard Cite

show abstract

“…El cultivo de células animales in vitro constituye una de las técnicas más eficaces para la investigación de procesos celulares cuyos resultados han generado gran impacto en el diagnóstico y tratamiento de diversas enfermedades (20)…”

Section: Modelos In Vitro Para Estudios De Cáncer De Vías Digestivas ...unclassified

Estrategias para evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas

Ferrebuz

Sánchez-Quitian

Salcedo

et al. 2022

Revista Investig. Salud Univ. Boyacá

View full text Add to dashboard Cite

Introducción: Los polifenoles son compuestos que se encuentran naturalmente en los alimentos como frutas, verduras, té, vino y chocolates, se le atribuye beneficios a la salud humana por su capacidad antioxidante, entre otras. El cáncer las vías digestivas se encuentran entre la tercera y quinta causa de muerte para la población, aumentando el interés por realizar los estudios dirigidos a encontrar compuestos polifenólicos que ayuden en su prevención o tratamiento. Objetivo: identificar las estrategias disponibles para la evaluación de polifenoles en células de cáncer de vías digestivas. Metodología: Se realizó una búsqueda de literatura en bases de datos como Ovid, Pubmed, Science Direct y Elsevier Journal y se seleccionaron artículos donde se reporta el efecto biológico de los polifenoles sobre líneas celulares de cáncer de vías digestivas publicados entre el 2012 y 2022. Resultados: Actualmente estudios reportan el uso de un buen número de líneas celulares como modelos in vitro para estudios de polifenoles en cáncer resaltando las líneas AGS y HT-29, además de técnicas para la caracterización de los polifenoles como el ensayo 2,2-Difenil-I-Picril Hidrazilo (DPPH), sin embargo, para evaluar el efecto biológico se identifican diversas pruebas que deben ser analizadas antes de su implementación. Conclusiones: En la literatura se identifica que existen varias alternativas y estrategias para la evaluación de extractos vegetales en cultivos in vitro de cáncer de vías digestivas, no obstante, antes de pasar al diseño experimental deben tenerse en cuenta una serie de consideraciones para garantizar la utilidad de los resultados.

show abstract

“…Nevertheless, this cytokine did not stimulate the migration or tube formation of HMEC-1 cells[ 44 ]. Assays performed to evaluate cell motility and tube formation on a matrix are widely used to study angiogenesis in vitro [ 70 ]. However, it would be necessary to carry out ex vivo or in vivo assays to study further if PTHrP has or not a direct action on endothelial cells with the aim to promote angiogenesis.…”

Section: Effects Of Pthrp On Tumor-associated Angiogenesismentioning

confidence: 99%

Involvement of parathyroid hormone-related peptide in the aggressive phenotype of colorectal cancer cells

Díaz¹,

Carriere²,

Martín³

et al. 2021

WJG

View full text Add to dashboard Cite

Colorectal cancer (CRC) remains one of the leading causes of mortality from malignant diseases worldwide. In general terms, CRC presents high heterogeneity due to the influence of different genetic and environmental factors; also, the neoplastic cells are strongly influenced by the extracellular matrix and several surrounding cells, known together as the tumor microenvironment (TME). Bidirectional communication takes place between the tumor and the TME through the release of autocrine and paracrine factors. Parathyroid hormone-related peptide (PTHrP) is a cytokine secreted by a wide variety of tissues and is able to regulate several cellular functions both in physiological as well as in pathological processes. It exerts its effects as a paracrine/autocrine factor, although its mode of action is mainly paracrine. It has been shown that this peptide is expressed by several tumors and that the tumor secretion of PTHrP is responsible for the malignant humoral hypercalcemia. Eight years ago, when our research group started studying PTHrP effects in the experimental models derived from intestinal tumors, the literature available at the time addressing the effects of PTHrP on colorectal tumors was limited, and no articles had been published regarding to the paracrine action of PTHrP in CRC cells. Based on this and on our previous findings regarding the role of PTH in CRC cells, our purpose in recent years has been to explore the role of PTHrP in CRC. We analyzed the behavior of CRC cells treated with exogenous PTHrP, focalizing in the study of the following events: Survival, cell cycle progression and proliferation, migration, chemoresistance, tumor-associated angiogenesis, epithelial to mesenchymal transition program and other events also associated with invasion, such us the induction of cancer stem cells features. This work summarizes the major findings obtained by our investigation group using in vitro and in vivo CRC models that evidence the participation of PTHrP in the acquisition of an aggressive phenotype of CRC cells and the molecular mechanisms involved in these processes. Recently, we found that this cytokine induces this malignant behavior not only by its direct action on these intestinal cells but also through its influence on cells derived from TME, promoting a communication between CRC cells and surrounding cells that contributes to the molecular and morphological changes observed in CRC cells. These investigations establish the basis for our next studies in order to address the clinical applicability of our findings. Recognizing the factors and mechanisms that promote invasion in CRC cells, evasion to the cytotoxic effects of current CRC therapies and thus metastasis is decisive for the identification of new markers with the potential to improve early diagnosis and/or to predict prognosis, to predetermine drug resistance and to provide treatment guidelines that include targeted therapies for this disease.

show abstract

An Overview of In Vitro, In Vivo, and Computational Techniques for Cancer‐Associated Angiogenesis Studies

Cited by 9 publications

References 105 publications

Involvement of Resveratrol against Brain Cancer: A Combination Strategy with a Pharmaceutical Approach

Involvement of Resveratrol against Brain Cancer: A Combination Strategy with a Pharmaceutical Approach

Estrategias para evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas

Involvement of parathyroid hormone-related peptide in the aggressive phenotype of colorectal cancer cells

Contact Info

Product

Resources

About