An overview of methods for assessment of free radical activity in biology

Jackson, Malcolm J.

doi:10.1017/s0029665199001317

Cited by 57 publications

(39 citation statements)

References 22 publications

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“…Work from our laboratory has previously shown that patients with type 1 diabetes are susceptible to increased blood levels of free radicals as documented by EPR spectroscopy, the most direct method of measuring free radical species [27]. The present study has consistently confirmed this observation and in addition demonstrates that ascorbate prophylaxis attenuates exercise-induced oxidative stress.…”

Section: Discussionsupporting

confidence: 84%

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

et al. 2008

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Aims/hypothesis Patients with type 1 diabetes mellitus are more susceptible than healthy individuals to exercise-induced oxidative stress and vascular endothelial dysfunction, which has important implications for the progression of disease. Thus, in the present study, we designed a randomised doubleblind, placebo-controlled trial to test the original hypothesis that oral prophylaxis with vitamin C attenuates rest and exercise-induced free radical-mediated lipid peroxidation in type 1 diabetes mellitus. Methods All data were collected from hospitalised diabetic patients. The electron paramagnetic resonance spectroscopic detection of spin-trapped α-phenyl-tert-butylnitrone (PBN) adducts was combined with the use of supporting markers of lipid peroxidation and non-enzymatic antioxidants to assess exercise-induced oxidative stress in male patients with type 1 diabetes (HbA 1c 7.9±1%, n=12) and healthy controls (HbA 1c 4.6±0.5%, n=14). Following participant randomisation using numbers in a sealed envelope, venous blood samples were obtained at rest, after a maximal exercise challenge and before and 2 h after oral ingestion of 1 g ascorbate or placebo. Participants and lead investigators were blinded to the administration of either placebo or ascorbate treatments. Primary outcome was the difference in changes in free radicals following ascorbate ingestion. Results Six diabetic patients and seven healthy control participants were randomised to each of the placebo and ascorbate groups. Diabetic patients (n=12) exhibited an elevated concentration of PBN adducts (p<0.05 vs healthy, n=14), which were confirmed as secondary, lipid-derived oxygen-centred alkoxyl (RO·) radicals (a nitrogen =1.37 mT and aβ hydrogen =0.18 mT). Lipid hydroperoxides were also selectively elevated and associated with a depression of retinol and lycopene (p<0.05 vs healthy). Vitamin C supplementation increased plasma vitamin C concentration to a similar degree in both groups (p<0.05 vs presupplementation) and attenuated the exercise-induced oxidative stress response (p<0.05 vs healthy).There were no selective treatment differences between groups in the primary outcome variable. Conclusions/interpretation These findings are the first to suggest that oral vitamin C supplementation provides an Diabetologia

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Section: Discussionsupporting

confidence: 84%

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

et al. 2008

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“…MDA, a lipid peroxidation end product, is an indicator of oxidative stress (Jackson, 1999). In this study, the MDA levels of the brain were examined by spectrophotometric assay.…”

Section: L-nbp Decreased Oxidative Stress In 3xtg-ad Micementioning

confidence: 99%

L-3-n-Butylphthalide Improves Cognitive Impairment and Reduces Amyloid- in a Transgenic Model of Alzheimer's Disease

Peng¹,

Sun²,

Hon³

et al. 2010

Journal of Neuroscience

126

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Alzheimer's disease (AD) is an age-related, progressive neurodegenerative disorder that occurs gradually and results in memory, behavior, and personality changes. L-3-n-butylphthalide (L-NBP), an extract from seeds of Apium graveolens Linn (Chinese celery), has been demonstrated to have neuroprotective effects on ischemic, vascular dementia, and amyloid-␤ (A␤)-infused animal models. In the current study, we examined the effects of L-NBP on learning and memory in a triple-transgenic AD mouse model (3xTg-AD) that develops both plaques and tangles with aging, as well as cognitive deficits. Ten-month-old 3xTg-AD mice were given 15 mg/kg L-NBP by oral gavage for 18 weeks. L-NBP treatment significantly improved learning deficits, as well as long-term spatial memory, compared with vehicle control treatment. L-NBP treatment significantly reduced total cerebral A␤ plaque deposition and lowered A␤ levels in brain homogenates but had no effect on fibrillar A␤ plaques, suggesting preferential removal of diffuse A␤ deposits. Furthermore, we found that L-NBP markedly enhanced soluble amyloid precursor protein secretion (␣APPs), ␣-secretase, and PKC␣ expression but had no effect on steady-state full-length APP. Thus, L-NBP may direct APP processing toward a non-amyloidogenic pathway and preclude A␤ formation in the 3xTg-AD mice. The effect of L-NBP on regulating APP processing was further confirmed in neuroblastoma SK-N-SH cells overexpressing wild-type human APP 695 (SK-N-SH APPwt). L-NBP treatment in 3xTg-AD mice also reduced glial activation and oxidative stress compared with control treatment. L-NBP shows promising preclinical potential as a multitarget drug for the prevention and/or treatment of Alzheimer's disease.

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“…É importante salientar que as substâncias produzidas pela peroxidação são instáveis e existe ausência de níveis detectáveis em alguns fluidos e tecidos (Jackson, 1999).…”

Section: Determinação Da Lipoperoxidação Pela Produção De Tbars Nos Tunclassified

Propriedades antioxidantes em subproduto do pedúnculo de caju (Anacardium occidentale L.): efeito sobre a lipoperoxidação e o perfil de ácidos graxos poliinsaturados em ratos

Broinizi

Andrade-Wartha

Silva

et al. 2008

Rev. Bras. Cienc. Farm.

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An overview of methods for assessment of free radical activity in biology

Cited by 57 publications

References 22 publications

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

L-3-n-Butylphthalide Improves Cognitive Impairment and Reduces Amyloid- in a Transgenic Model of Alzheimer's Disease

Propriedades antioxidantes em subproduto do pedúnculo de caju (Anacardium occidentale L.): efeito sobre a lipoperoxidação e o perfil de ácidos graxos poliinsaturados em ratos

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