An overview on display systems (phage, bacterial, and yeast display) for production of anticancer antibodies; advantages and disadvantages

Mahdavi, Seyedeh Zahra Bahojb; Oroojalian, Fatemeh; Eyvazi, Shirin; Hejazi, Maryam; Baradaran, Behzad; Pouladi, Nasser; Tohidkia, Mohammad Reza; Mokhtarzadeh, Ahad

doi:10.1016/j.ijbiomac.2022.03.113

Cited by 59 publications

(33 citation statements)

References 243 publications

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“…Although antibodies have been widely used as targeting probes, they have several limitations for in vivo imaging, including difficult modification, low binding efficiency, and slow metabolism. 14,15 In contrast, antibody mimetic peptides have gained increasing attention in MR-based molecular imaging due to their fabulous features including easy synthesis, convenient modification and fast binding. 16 We herein report the construction of a collagen-targeting MRI peptide contrast agent for in vivo imaging of hepatic fibrosis (Fig.…”

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confidence: 99%

A robust collagen-targeting MRI peptide contrast agent for in vivo imaging of hepatic fibrosis

et al. 2023

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confidence: 99%

A robust collagen-targeting MRI peptide contrast agent for in vivo imaging of hepatic fibrosis

et al. 2023

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“…In addition, fusion and incorporation of the phage coat protein with its fusion partner occur in the periplasm. This feature is an advantage for several proteins, including secreted heterologous proteins but a disadvantage for cytoplasmic proteins or molecules that have an inhibitory effect on the secretion process, which cannot pass through the inner membrane and cannot be transported to the periplasm to complete the folding and be finally displayed correctly on the phage surface [ 81 ]. To overcome the deficiency of M13, scientists have focused on virulent phage T7 in the field of pathogenic microorganisms.…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

Phage Display-Derived Peptides and Antibodies for Bacterial Infectious Diseases Therapy and Diagnosis

Zhao

Nie

et al. 2023

Molecules

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The emergence of antibiotic-resistant-bacteria is a serious public health threat, which prompts us to speed up the discovery of novel antibacterial agents. Phage display technology has great potential to screen peptides or antibodies with high binding capacities for a wide range of targets. This property is significant in the rapid search for new antibacterial agents for the control of bacterial resistance. In this paper, we not only summarized the recent progress of phage display for the discovery of novel therapeutic agents, identification of action sites of bacterial target proteins, and rapid detection of different pathogens, but also discussed several problems of this technology that must be solved. Breakthrough in these problems may further promote the development and application of phage display technology in the biomedical field in the future.

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“…Additionally, biological display systems ensure strict expression regulation and easy library amplification at low cost as well as rapid information decoding of candidates via polymerase chain reaction (PCR) and sequencing. Currently, representative display platforms are phage, bacteria, yeast, ribosome, mRNA, and mammalian cell display systems [17,18]. More importantly, the microplates and microarray chips of libraries or target molecules can be integrated with the above physical detection instruments to establish a high-throughput screening platform, which enables rapid and automatic screening of thousands of samples for affinity toward one or more molecules in parallel [19].…”

Section: High-throughput Screening Using Biological Display Systemsmentioning

confidence: 99%

High‐throughput screening and biological display technology: Applications in molecular imaging

2023

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Molecular imaging plays important roles in many fields, including disease diagnosis, therapeutic efficacy evaluation, intraoperative imaging guidance, drug metabolism monitoring, and patient selection for appropriate treatment. As a key component, the targeting ligand determines the specificity, affinity, and in vivo performance of molecular imaging probes. In this review, high‐throughput screening and biological display platforms for the discovery of ligands applicable to molecular imaging are briefly reviewed. Basic information on ligand development for molecular imaging is first introduced, followed by a presentation of various selection platforms and typical or iterative cases. The features, advantages, limitations, and application scope of screening and display platforms are compared and discussed. Last, a basic selection strategy and a perspective for protein‐based ligands are provided.

show abstract

An overview on display systems (phage, bacterial, and yeast display) for production of anticancer antibodies; advantages and disadvantages

Cited by 59 publications

References 243 publications

A robust collagen-targeting MRI peptide contrast agent for in vivo imaging of hepatic fibrosis

A robust collagen-targeting MRI peptide contrast agent for in vivo imaging of hepatic fibrosis

Phage Display-Derived Peptides and Antibodies for Bacterial Infectious Diseases Therapy and Diagnosis

High‐throughput screening and biological display technology: Applications in molecular imaging

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