An overview on molecular markers for detection of ochratoxigenic fungi in coffee neans

Fungaro, Maria Helena Pelegrinelli; Sartori, Daniele

doi:10.1590/s1516-89132009000700001

Cited by 7 publications

(3 citation statements)

References 30 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results are partly agreed with that of Sartori et al (2006) found ochratoxin gene amplicon band at 372 bp. While, Fungaro and sartori (2009) and Schmidt et al (2004) found that A. ochraceous gene was expression OCR-A gene at 260 bp. The obvious variance in the above result may be due to technical aspects.…”

Section: Expression Of the Structure Genesmentioning

confidence: 95%

Detection of aflatoxins by HPLC and the expression of biosynthetic nor-1 gene of aflatoxin and ocrA gene of ochratoxin

Hassan

Nada

Elsayed

2015

Benha Veterinary Medical Journal

View full text Add to dashboard Cite

Due to the high toxicity of aflatoxins and its effects on public health and animal wealth, food and feed. The determination of mycotoxins in commodities is the only way of control. The identification of aflatoxigenic Aspergillus isolates through detection of nor-1 gene was tried. Ochratoxin has been widely detected in cereal-derived products. Its production by A. ochraceous and its biosynthetic gene ocrA was evaluated. For this purpose, 500 different cereal samples were collected from different markets in Sharkia Province. Five strains of Aspergillus spp. have been screened for their ability to produce aflatoxins after cultivation in Czapek,s Dox media. Aflatoxin B1was detected in average amount 4.9-200 ng/µl through using HPLC technique. A Real time PCR (RT-PCR) technique was applied to determine the gene expression of structural nor-1, which catalyzed the first step in aflatoxins biosynthetic pathway.

show abstract

Section: Expression Of the Structure Genesmentioning

confidence: 95%

Detection of aflatoxins by HPLC and the expression of biosynthetic nor-1 gene of aflatoxin and ocrA gene of ochratoxin

Hassan

Nada

Elsayed

2015

Benha Veterinary Medical Journal

View full text Add to dashboard Cite

show abstract

“…For plant phytopathogens, morphological characterization is still the most used method of fungal identification to this day (Carnielli-Queiroz et al, 2019). However, PCR is already being used to identify the species of fungi in food samples, causing a difference in the final quantity of the product (Fungaro & Sartori, 2009). Another example is the detection of the fungus that causes the disease in the pine.…”

Section: F Verticillioides Detection On Maize Seeds With Different In...mentioning

confidence: 99%

Detection and transmission of Fusarium verticillioides in corn seeds according to the plant stage

et al. 2021

View full text Add to dashboard Cite

The objective of this study was to evaluate the transmissibility of F. verticillioides, in corn seeds, when inoculated in different phenological stages. The plants were inoculated with the pathogen at the following stages: V7, V9, R1, R2, R4, and R6. The experiments were conducted in pots, in a completely randomized design, with six replications. The fungus infection rate in the seeds was verified through the health test (Blotter Test). The significance of the contrast between the phenological stages and in the two experiments (rainy season and dry season), and productivity showed plant-seed transmission of F. verticillioides in all phenological stages. The PCR technique was able to reveal up to the lowest level, 1% incidence of F. verticillioides in corn seeds. There was a significant difference between the times of plant inoculation and the incidence of the fungus in the seeds. Plants inoculated in the reproductive phase transmitted F. verticillioides for seeds with the highest rate of infection, in the two experiments, in the rainy season (V7 - 48; V9 - 46; R1 - 77.33; R2 - 84; R4 - 96; R6 - 96.67; and Test - 14.67%), and dry season (V7 - 46.67; V9 - 66; R1 - 75.33; R2 - 95.33; R4 - 98; and R6 - 97.33%). In this way, the present work demonstrated that in the reproductive phenological stages R2, R4, and R6, the corn plant was more susceptible to infection with F. verticillioides, and that it also resulted in a decrease in grain productivity.

show abstract

“…Aspergillus and Penicillium species are the major producers of ochratoxins. Ochratoxin A (OTA) is a mycotoxin that is receiving increasing attention worldwide because of its severe nephrotoxicity [8].…”

Section: Introductionmentioning

confidence: 99%

Molecular Screening of Fungal Isolates of Palm Oil from South Eastern Nigeria for Aflatoxin and Ochratoxin Biosynthetic Genes Using Multiplex Polymerase Chain Reaction (mPCR)

Nwachukwu

Amadi

Ogwo

et al. 2019

MRJI

View full text Add to dashboard Cite

In Nigeria and many other developing countries of the world, the incidence of mycotoxin- contamination of foods and food products has attracted attention and stirred a lot of concern for food safety. This work aims at detection of aflatoxigenic and ochratoxigenic synthetic genes from fungal isolates of palm oil as a veritable means for the evaluation of foods for possible mycotoxin contamination. In this study, fungal isolates from palm oil samples collected from the five states of South-east geopolitical zone in Nigeria were screened for aflatoxin and ochratoxin biosynthetic genes using Multiplex Polymerase Chain Reaction (mPCR). The assay relied on three sets of primers that amplify aflatoxgenic Aspergillus, ochratoxigenic Aspergillus and Penicillium species under optimized PCR conditions. Optimum multiplex PCR assay was standardized for simultaneous detection of toxigenic Aspergillus and ochratoxin producing Penicillium species targeting AflR, AflS and pks genes involved in aflatoxin and ochratoxin metabolic pathways respectively. AflR primer pair gave specific amplification for aflatoxigenic A. flavus but did not give amplification for A. niger and P. chrysogenum. While AflS and pks gave amplification for only aflatoxigenic and ochratoxigenic A. niger and P. chrysogenum. In the evaluation and monitoring of mycotoxin-producing fungi during the processing of food and feed commodities, Multiplex PCR approach could be a veritable tool to supplement the conventional analytical techniques.

show abstract

An overview on molecular markers for detection of ochratoxigenic fungi in coffee neans

Abstract: ABSTRACT

Cited by 7 publications

References 30 publications

Detection of aflatoxins by HPLC and the expression of biosynthetic nor-1 gene of aflatoxin and ocrA gene of ochratoxin

Detection of aflatoxins by HPLC and the expression of biosynthetic nor-1 gene of aflatoxin and ocrA gene of ochratoxin

Detection and transmission of Fusarium verticillioides in corn seeds according to the plant stage

Molecular Screening of Fungal Isolates of Palm Oil from South Eastern Nigeria for Aflatoxin and Ochratoxin Biosynthetic Genes Using Multiplex Polymerase Chain Reaction (mPCR)

Contact Info

Product

Resources

About