An ovine chitinase-like molecule, chitinase-3 like-1 (YKL-40), is upregulated in the abomasum in response to challenge with the gastrointestinal nematode, Teladorsagia circumcincta

Knight, Pamela; Pate, Judith A.; Smith, William D.; Miller, H. R. P.

doi:10.1016/j.vetimm.2007.07.005

Cited by 10 publications

(9 citation statements)

References 32 publications

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“…The chitinase-like molecule, CHI3L1 , has been shown to be up-regulated in the abomasum of sheep in response to T. circumcincta challenge of previously infected animals [55]. CHIA expression levels were also examined in the same study, and while expression was observed up-regulation of transcripts was not significant.…”

Section: Discussionmentioning

confidence: 99%

Signatures of selection in sheep bred for resistance or susceptibility to gastrointestinal nematodes

et al. 2014

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BackgroundGastrointestinal nematodes are one of the most serious causes of disease in domestic ruminants worldwide. There is considerable variation in resistance to gastrointestinal nematodes within and between sheep breeds, which appears to be due to underlying genetic diversity. Through selection of resistant animals, rapid genetic progress has been demonstrated in both research and commercial flocks. Recent advances in genome sequencing and genomic technologies provide new opportunities to understand the ovine host response to gastrointestinal nematodes at the molecular level, and to identify polymorphisms conferring nematode resistance.ResultsDivergent lines of Romney and Perendale sheep, selectively bred for high and low faecal nematode egg count, were genotyped using the Illumina® Ovine SNP50 BeadChip. The resulting genome-wide SNP data were analysed for selective sweeps on loci associated with resistance or susceptibility to gastrointestinal nematode infection. Population differentiation using FST and Peddrift revealed sixteen regions, which included candidate genes involved in chitinase activity and the cytokine response. Two of the sixteen regions identified were contained within previously identified QTLs associated with nematode resistance.ConclusionsIn this study we identified fourteen novel regions associated with resistance or susceptibility to gastrointestinal nematodes. Results from this study support the hypothesis that host resistance to internal nematode parasites is likely to be controlled by a number of loci of moderate to small effects.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-637) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Signatures of selection in sheep bred for resistance or susceptibility to gastrointestinal nematodes

et al. 2014

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“…Previously described work has established that there were significant differences in anti-parasite responses between the "naïve" and "immune" groups used in this study [4,11]. Samples of host material collected post-mortem included abomasal fold (anterior to the fundic region) for RNA extraction [17]. RNA was extracted as described previously [17] and assessed for quality and quantity using an Agilent 2100 Bioanalyzer (Agilent Technologies UK Ltd, Edinburgh, U.K.) according to ARK-Genomics standard protocols [18].…”

Section: Methodsmentioning

confidence: 99%

“…Samples of host material collected post-mortem included abomasal fold (anterior to the fundic region) for RNA extraction [17]. RNA was extracted as described previously [17] and assessed for quality and quantity using an Agilent 2100 Bioanalyzer (Agilent Technologies UK Ltd, Edinburgh, U.K.) according to ARK-Genomics standard protocols [18]. …”

Section: Methodsmentioning

confidence: 99%

Novel gene expression responses in the ovine abomasal mucosa to infection with the gastric nematode Teladorsagia circumcincta

Knight

Griffith

Pemberton

et al. 2011

Vet Res

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Infection of sheep with the gastric nematode Teladorsagia circumcincta results in distinct Th2-type changes in the mucosa, including mucous neck cell and mast cell hyperplasia, eosinophilia, recruitment of IgA/IgE producing cells and neutrophils, altered T-cell subsets and mucosal hypertrophy. To address the protective mechanisms generated in animals on previous exposure to this parasite, gene expression profiling was carried out using samples of abomasal mucosa collected pre- and post- challenge from animals of differing immune status, using an experimental model of T. circumcincta infection. Recently developed ovine cDNA arrays were used to compare the abomasal responses of sheep immunised by trickle infection with worm-naïve sheep, following a single oral challenge of 50 000 T. circumcincta L3. Key changes were validated using qRT-PCR techniques. Immune animals demonstrated highly significant increases in levels of transcripts normally associated with cytotoxicity such as granulysin and granzymes A, B and H, as well as mucous-cell derived transcripts, predominantly calcium-activated chloride channel 1 (CLCA1). Challenge infection also induced up-regulation of transcripts potentially involved in initiating or modulating the immune response, such as heat shock proteins, complement factors and the chemokine CCL2. In contrast, there was marked infection-associated down-regulation of gene expression of members of the gastric lysozyme family. The changes in gene expression levels described here may reflect roles in direct anti-parasitic effects, immuno-modulation or tissue repair. (Funding; DEFRA/SHEFC (VT0102) and the BBSRC (BB/E01867X/1)).

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“…Chitinase-like proteins are a characteristic feature of multiple helminth infection models [16], [17]. Chitinase 3-like 1 (CHI3L1), also known as human cartilage glycoprotein 39 (HCgp-39), and YKL-40, is a human chitinase-like chitin-binding lectin with no chitinase enzymatic activity [16], [17].…”

Section: Introductionmentioning

confidence: 99%

“…Chitinase 3-like 1 (CHI3L1), also known as human cartilage glycoprotein 39 (HCgp-39), and YKL-40, is a human chitinase-like chitin-binding lectin with no chitinase enzymatic activity [16], [17]. It is expressed by numerous cell types and has been associated with collagen and extra-cellular matrix formation [18] as well as with a wide range of diseases characterised by inflammation and tissue remodelling including asthma, arthritis, numerous cancers and liver fibrosis [19], [20], [21].…”

Section: Introductionmentioning

confidence: 99%

Chitinase 3-Like 1 Protein Levels Are Elevated in Schistosoma haematobium Infected Children

et al. 2012

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BackgroundCurrently there are few studies characterising the nature and aetiology of human schistosome-related inflammatory processes. The aim of this study was to determine the relationship between Chitinase 3-like 1 (CHI3L1), also known as YKL-40, a molecule associated with inflammatory processes, and schistosome infection, morbidity and systemic cytokine levels.MethodsSerological levels of CHI3L1 and a panel of cytokines (IFN-y, IL-4/5/6/9/10/13 and 17) were measured in two Zimbabwean populations resident in a high and low schistosome infection area. CHI3L1 levels were related to schistosome infection, haematuria status and cytokine levels after allowing for confounding variables. The effect of antihelminthic treatment with praziquantel on CHI3L1 levels was determined in 246 participants 6 weeks post-treatment.ResultsCHI3L1 levels increased with age in both areas but were significantly higher in the high infection areas compared to the low infection area. CHI3L1 levels were also higher in infected compared to uninfected individuals with this difference being significant in the youngest age group. Curative antihelminthic treatment resulted in a significant decrease in CHI3L1 levels. Of the cytokines, only IL-10 and IL-17 had a significant association with CHI3L1 levels, and this association was negative.ConclusionsSerum CHI3L1 levels differ between infected and uninfected people before and after antihelminthic treatment. The greatest difference occurs in the youngest age group, in keeping with the period when schistosome-related pathological processes are initiated. Following from previous studies in non-infectious diseases showing that CHI3L1 is a biomarker for the inflammatory process, this study suggests that the potential for CHI3L1 as a biomarker for schistosome-related pathology should be explored further.

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An ovine chitinase-like molecule, chitinase-3 like-1 (YKL-40), is upregulated in the abomasum in response to challenge with the gastrointestinal nematode, Teladorsagia circumcincta

Cited by 10 publications

References 32 publications

Signatures of selection in sheep bred for resistance or susceptibility to gastrointestinal nematodes

Signatures of selection in sheep bred for resistance or susceptibility to gastrointestinal nematodes

Novel gene expression responses in the ovine abomasal mucosa to infection with the gastric nematode Teladorsagia circumcincta

Chitinase 3-Like 1 Protein Levels Are Elevated in Schistosoma haematobium Infected Children

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