An RFLP map of diploid Hordeum bulbosum L. and comparison with maps of barley (H. vulgare L.) and wheat (Triticum aestivum L.)

Salvo-Garrido, Haroldo; Laurie, D. A.; Jaffe, Benjamin D; Snape, J. W.

doi:10.1007/s001220100707

Cited by 26 publications

(22 citation statements)

References 39 publications

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“…It is apparent that the ratio of genetic to physical distance is highly distorted, and that the degree of distortion is more conspicuous in H. bulbosum than in barley. This is consistent with the data previously obtained from the comparison of the linkage maps between H. bulbosum and barley (JaVé et al 2000;Salvo-Garrido et al 2001). …”

Section: Fine Mapping Of S Locus-linked Markerssupporting

confidence: 93%

Molecular and genetic characterization of the S locus in Hordeum bulbosum L., a wild self-incompatible species related to cultivated barley

et al. 2008

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Gametophytic self-incompatibility (GSI) in the grasses is controlled by a distinct two-locus genetic system governed by the multiallelic loci S and Z. We have employed diploid Hordeum bulbosum as a model species for identifying the self-incompatibility (SI) genes and for elucidating the molecular mechanisms of the two-locus SI system in the grasses. In this study, we attempted to identify S haplotype-specific cDNAs expressed in pistils and anthers at the flowering stage in H. bulbosum, using the AFLP-based mRNA fingerprinting (AMF, also called cDNA-AFLP) technique. We used the AMF-derived DNA clones as markers for fine mapping of the S locus, and found that the locus resided in a chromosomal region displaying remarkable suppression of recombination, encompassing a large physical region. Furthermore, we identified three AMF-derived markers displaying complete linkage to the S locus, although they showed no significant homology with genes of known functions. Two of these markers showed expression patterns that were specific to the reproductive organs (pistil or anther), suggesting that they could be potential candidates for the S gene.

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Section: Fine Mapping Of S Locus-linked Markerssupporting

confidence: 93%

Molecular and genetic characterization of the S locus in Hordeum bulbosum L., a wild self-incompatible species related to cultivated barley

et al. 2008

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“…Pr1 was located in a linkage group together with markers Xpsr148 and Xscm28. These anchor markers were previously mapped in the distal part of rye chromosome 6RL (Devos et al 1993;Saal and Wricke 1999;Korzun et al 2001) as well as barley chromosome 7HL (Laurie et al 1995;Salvo-Garrido et al 2001). The distant proximal position of Pr1 from these two markers, together with its close linkage to Aco1, suggests that Pr1 is located on the 6L segment.…”

Section: Discussionmentioning

confidence: 99%

Leaf-rust resistance in rye (Secale cereale L.). 1. Genetic analysis and mapping of resistance genes Pr1 and Pr2

et al. 2003

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Genetic analysis of resistance to leaf rust in rye (Puccinia recondita f. sp. secalis) led to the identification of two dominant resistance genes, Pr1 and Pr2. Both genes proved to be effective against a local leaf-rust population as well as a subset of single-pustule isolates (SPIs) the latter of which comprised SPIs with very high virulence complexity. Resistance conferred by Pr1 and Pr2 was expressed in detached-leaf tests of seedlings as well as in field tests of adult plants. Molecular marker analysis allowed us to map Pr1 in the proximal part of rye chromosome 6RL, whereas Pr2 was assigned to the distal part of chromosome 7RL. These results are discussed in view of homoeology relationships among Triticeae. A proposal is submitted for the designation of resistance genes to rye leaf rust which would avoid interference with existing gene-symboling in respect to wheat leaf-rust resistances introgressed from rye into wheat or triticale.

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“…Genes were then mapped in barley or, where polymorphism was lacking, Hordeum bulbosum or rye (Fig. 3), utilizing the known colinearity of their maps (Salvo-Garrido et al, 2001 andDevos et al, 1993, respectively). This showed that barley CO-like genes were present on several chromosomes, with three on chromosome 6H (HvCO2, 5, and 7) and three on chromosome 7H (HvCO1, 6, and 8).…”

Section: Genetic Mapping Of Barley Constans-like Genesmentioning

confidence: 99%

The Evolution of CONSTANS-Like Gene Families in Barley, Rice, and Arabidopsis

et al. 2003

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The CO (CONSTANS) gene of Arabidopsis has an important role in the regulation of flowering by photoperiod. CO is part of a gene family with 17 members that are subdivided into three classes, termed Group I to III here. All members of the family have a CCT (CO, CO-like, TOC1) domain near the carboxy terminus. Group I genes, which include CO, have two zinc finger B-boxes near the amino terminus. Group II genes have one B-box, and Group III genes have one B-box and a second diverged zinc finger. Analysis of rice (Oryza sativa) genomic sequence identified 16 genes (OsA-OsP) that were also divided into these three groups, showing that their evolution predates monocot/dicot divergence. Eight Group I genes (HvCO1-HvCO8) were isolated from barley (Hordeum vulgare), of which two (HvCO1 and HvCO2) were highly CO like. HvCO3 and its rice counterpart (OsB) had one B-box that was distantly related to Group II genes and was probably derived by internal deletion of a two B-box Group I gene. Sequence homology and comparative mapping showed that HvCO1 was the counterpart of OsA (Hd1), a major determinant of photoperiod sensitivity in rice. Major genes determining photoperiod response have been mapped in barley and wheat (Triticum aestivum), but none corresponded to CO-like genes. Thus, selection for variation in photoperiod response has affected different genes in rice and temperate cereals. The peptides of HvCO1, HvCO2 (barley), and Hd1 (rice) show significant structural differences from CO, particularly amino acid changes that are predicted to abolish B-box2 function, suggesting an evolutionary trend toward a one-B-box structure in the most CO-like cereal genes.The control of flowering by photoperiod is an important adaptive characteristic in plants. Studies of the model dicot Arabidopsis have shown that the CO (CONSTANS) gene, isolated by Putterill et al. (1995), has an important role in the photoperiod pathway, which is one of four regulatory pathways controlling the timing of flowering (for review, see Mouradov et al., 2002;Simpson and Dean, 2002). CO acts between the circadian clock and genes controlling meristem identity (Samach et al., 2000;Suárez-Ló pez et al., 2001). In Arabidopsis, CO belongs to a family of 17 putative transcription factors defined by two conserved domains (Putterill et al., 1995;Robson et al., 2001). The first is a zinc finger region near the amino terminus that resembles B-boxes, which regulate protein-protein interactions in several animal transcription factors (Borden, 1998;Torok and Elkin, 2000). The second is a region of 43 amino acids near the carboxy terminus termed the CCT (CO, CO-like, TOC1) domain (Strayer et al., 2000;Robson et al., 2001). Studies using green fluorescent protein fusions show that the CCT domain is involved in nuclear localization of the CO protein but must have an additional role because the late-flowering co-7 mutant, which has an altered CCT domain, correctly localizes the protein .Previous analysis of CO-like genes in Arabidopsis showed that the family is subdivided int...

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An RFLP map of diploid Hordeum bulbosum L. and comparison with maps of barley (H. vulgare L.) and wheat (Triticum aestivum L.)

Cited by 26 publications

References 39 publications

Molecular and genetic characterization of the S locus in Hordeum bulbosum L., a wild self-incompatible species related to cultivated barley

Molecular and genetic characterization of the S locus in Hordeum bulbosum L., a wild self-incompatible species related to cultivated barley

Leaf-rust resistance in rye (Secale cereale L.). 1. Genetic analysis and mapping of resistance genes Pr1 and Pr2

The Evolution of CONSTANS-Like Gene Families in Barley, Rice, and Arabidopsis

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