An rGONS-based biosensor for simultaneous imaging of p53 and p21 mRNA in living cells

Fan, Jialong; Tong, Chunyi; Dang, Wenya; Yan, Qin; Liu, Xuanming; Liu, Bin; Wang, Wei

doi:10.1016/j.talanta.2019.05.087

Cited by 13 publications

(8 citation statements)

References 37 publications

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“…Herein, a reduced graphene oxide nanosheet (rGONS)–modified nanosystem is constructed for in situ and real-time p53 and p21 mRNA imaging by adsorbing the FAM-labeled p21 probe (P21) and Cy5-labeled p53 probe (P53). Once the two fluorescence probes hybridize with corresponding targets, the formation of DNA/RNA duplexes directly facilitates the release of probes from the rGONS surface and then restores the fluorescence signal ( Fan et al, 2019 ). Therefore, the nanosystem in situ reveals a p53 and p21 mRNA–related regulatory process, which is practicable for drug screening and therapy evaluation in clinics.…”

Section: The Application Of Nucleic Acid Probe–based Fluorescent Imaging In Cancer Diagnosis and Treatmentmentioning

confidence: 99%

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

Huang

Wang

et al. 2021

Front. Chem.

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It is well known that cancer incidence and death rates have been growing, but the development of cancer theranostics and therapeutics has been a challenging work. Recently, nucleic acid probe–based fluorescent sensing and imaging have achieved remarkable improvements in a variety of cancer management techniques, credited to their high sensitivity, good tolerance to interference, fast detection, and high versatility. Herein, nucleic acid probe–based fluorescent sensing and imaging are labeled with advanced fluorophores, which are essential for fast and sensitive detection of aberrant nucleic acids and other cancer-relevant molecules, consequently performing cancer early diagnosis and targeted treatment. In this review, we introduce the characteristics of nucleic acid probes, summarize the development of nucleic acid probe–based fluorescent sensing and imaging, and prominently elaborate their applications in cancer diagnosis and treatment. In discussion, some challenges and perspectives are elaborated in the field of nucleic acid probe–based fluorescent sensing and imaging.

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Section: The Application Of Nucleic Acid Probe–based Fluorescent Imaging In Cancer Diagnosis and Treatmentmentioning

confidence: 99%

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

Huang

Wang

et al. 2021

Front. Chem.

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“…Fana et al [40] also synthesized reduced graphene oxide-based biosensors to diagnose liver cancer. Graphene oxides are preferred as nanoparticles due to their electronic, mechanical and thermal stability.…”

Section: Biosensorsmentioning

confidence: 99%

“…High-sensitivity nonenzymatic hydrogen peroxide biosensor [37] Detection of single nucleotide polymorphism of tumor Gold probe with lateral flow strip biosensor [39] For simultaneous imaging of P53 and P21 mRNA in living cells An RGONS-based biosensor [40] Detection of lysozyme Aptamer-based electrochemical biosensors [41] Detection of arsenic and mercury Metallothionein-based biosensor [42] Detection of malachite green Free microcantilever based biosensor [43] Refractive index sensor A single-layer guided-mode resonant optical biosensor [44] The determination of 3methylquinoxaline-2-carboxylic acid Surface plasmon resonance biosensor [45] Detection of escherichia coli Label-free amperometric biosensor [46] Electrochemical detection of the human cancer biomarker…”

Section: Purpose Of Usage Biosensors Referencesmentioning

confidence: 99%

The Components of Functional Nanosystems and Nanostructures

Baysal¹

2020

Smart Nanosystems for Biomedicine, Optoelectronics and Catalysis

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The science of nanosystems is used in many fields such as medicine, biomedical, biotechnology, agriculture, environmental pollution control, cosmetics, optics, health, food, energy, textiles, automotive, communication technologies, agriculture, and electronics. Nanomaterials, nanostructures, and nanosystems have recently brought the most popular and innovative approaches to our lives. This new technology is based on the production of invisible particles and the production of new materials by controlling the atomic sequence of these particles. Nanotechnological studies are based on mimicking the principle of atomic sequence in nature. Using a combination of different disciplines, it finds application in almost every field of our lives. Nanospheres, nanorobots, biosensors, quantum dots, and biochips are the main components of nanoparticles. Many new diagnostic and treatment methods are being developed nano-dimensional.

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“…18 On the other hand, due to the complexity of the intracellular environment, the signal output in cells is weak to a certain extent, which makes it prone to generate a false positive signal. 19 Therefore, a more reliable platform for the real-time observation of mRNA in living cells would be of great significance.…”

Section: Introductionmentioning

confidence: 99%

“…Reduced graphene oxide nanosheet-assisted fluorescence probes were used to detect levels of p53 and p21 mRNA in HepG2 cells by Wang's group. 19 Tang et al employed a multicolored fluorescence nanoprobe based on AuNPs for simultaneously detecting three tumor-related mRNAs (c-myc mRNA, TK1 mRNA and GalNAc-T mRNA). 25 These two literature reports provide a novel method for mRNA detection by using different nanomaterials; however, the methods were complicated and needed more time to complete the response reaction.…”

Section: Introductionmentioning

confidence: 99%

Dynamic tracking of p21 mRNA in living cells by sticky-flares for the visual evaluation of the tumor treatment effect

Zhao

Dong

et al. 2022

Nanoscale

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An rGONS-based biosensor for simultaneous imaging of p53 and p21 mRNA in living cells

Cited by 13 publications

References 37 publications

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

The Components of Functional Nanosystems and Nanostructures

Dynamic tracking of p21 mRNA in living cells by sticky-flares for the visual evaluation of the tumor treatment effect

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