1993
DOI: 10.1007/bf01969382
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An SAR sequence containing 395 bp DNA fragment mediates enhanced, gene-dosage-correlated expression of a chimaeric heat shock gene in transgenic tobacco plants

Abstract: A 395 bp fragment located downstream from the soybean heat shock gene Gmhsp 17.6-L exhibits several characteristics of scaffold attachment region (SAR) sequences. It contains matrix consensus elements, a topoisomerase II binding sequence and it associates with the isolated nuclear scaffold of soybean in vitro. Chimaeric genes containing the SARL fragment either at one side (5' or 3') or at both sides of a heat shock promoter-regulated beta-glucuronidase reporter gene were constructed. A five- to nine-fold incr… Show more

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Cited by 85 publications
(50 citation statements)
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“…An interaction of C/EBP and HSF, bound to their respective cis elements, has been postulated to be required for maximum stress-induced transcription from human hsp70 promoters (Williams and Morimoto, 1990). In plants, there is evidence for the involvement of CCAAT-box elements, HSEs, and scaffold-attachment regions in stress-induced transcription (Rieping and Schö ffl, 1992;Schö ffl et al, 1993). Furthermore, STREs are known to activate transcription in response to a variety of stress conditions, especially heat (Siderius and Mager, 1997).…”
Section: Discussionmentioning
confidence: 99%
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“…An interaction of C/EBP and HSF, bound to their respective cis elements, has been postulated to be required for maximum stress-induced transcription from human hsp70 promoters (Williams and Morimoto, 1990). In plants, there is evidence for the involvement of CCAAT-box elements, HSEs, and scaffold-attachment regions in stress-induced transcription (Rieping and Schö ffl, 1992;Schö ffl et al, 1993). Furthermore, STREs are known to activate transcription in response to a variety of stress conditions, especially heat (Siderius and Mager, 1997).…”
Section: Discussionmentioning
confidence: 99%
“…The proteins are localized in different cell compartments, including the cytoplasm, the endoplasmic reticulum, and chloroplasts (Koning et al, 1992;Takahashi et al, 1992;Marrs et al, 1993;Schrö der et al, 1993;Krishna et al, 1995;Schmitz et al, 1996;Milioni and Hatzopoulos, 1997). The corresponding genes were shown to be specifically expressed during embryogenesis, pollen development (Marrs et al, 1993), and seed germination (Reddy et al, 1998) in young and rapidly divid-ing tissues such as shoot and root apices (Koning et al, 1992) and in flowers (Takahashi et al, 1992;Krishna et al, 1995).…”
mentioning
confidence: 99%
“…These features suggest a close relationship between enhancer and MAR activities. The colocalization of enhancer and MAR sequences has been shown previously for various genes, including the murine immunoglobulin (Forrester et al, 1999;Fernández et al, 2001), the bean phaseolin (van der Geest et al, 1994), the soybean Gmhsp17.6-L (Schöffl et al, 1993), and the tobacco RB7 (Allen et al, 1996) genes.…”
Section: The Pea Pete Enhancer Colocalizes With a Marmentioning
confidence: 52%
“…Figure 8A shows the preferential association of the 268-bp PetE enhancer with the nuclear matrix, whereas the pUBS vector fragment remained unbound. A control experiment with the soybean Gmhsp17.6-L MAR (Schöffl et al, 1993) Tobacco seedlings containing P-GUS were treated with 10 M trichostatin A (A) or 10 mM sodium butyrate (B) for 6 h. Nuclei were isolated from tobacco plants, cross-linked with formaldehyde, and immunoprecipitated with antibodies against acetylated histone H3 (AcH3 AB), nonacetylated histone H3 (nonAcH3 AB), acetylated histone H4 (AcH4 AB), or nonacetylated histone H4 (nonAcH4 AB). The amounts of PetE promoter sequence (analyzed with primer pairs C1 and C4) and the ϩ52 to ϩ586 region of uidA (analyzed with primer pairs C8 and C10) in the immunoprecipitates were quantified with PCR.…”
Section: The Pea Pete Enhancer Associates With the Nuclear Matrixmentioning
confidence: 99%
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