An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection

Abstract

A sensitive, precise and selective ultra-high performance liquid chromatography method coupled with triple-quadrupole mass spectrometry was developed and validated for the determination of trace amounts of sinomenine (ng/mL) in minute volumes of human plasma. Fifty microliter plasma samples were precipitated using methanol to extract sinomenine. Separation was carried out on a C18 column with a water and acetonitrile mobile phase gradient with formic acid as an additive. The mass spectrometry data were obtained in the positive ion mode, and the transition of multiple reactions was monitored at m/z 330.2→181.0 for sinomenine quantification. The working assay range for sinomenine was linear from 0.1173 to 15.02 ng/mL with the lower limit of quantification of 0.1173 ng/mL. The precision and accuracy of the method was less than 15% in intra-day and inter-day experiments with a matrix effect of less than 6.5%. After validation, the quantitative method was applied to analyze sinomenine levels in human plasma after transdermal delivery of the Zhengqing Fengtongning Injection. The results showed that some samples contained sinomenine within the concentration range 0.4131–4.407 ng/mL.

Controlled release of optimized electroporation enhances the transdermal efficiency of sinomenine hydrochloride for treating arthritis in vitro and in clinic

“…Quantification was performed via the multiple reaction monitoring of the transitions of m/z 330.2→239.1 and m/z 330.2→181.0 for SH34 and m/z 289.0→97.1 for IS. Collision energy was set at 26 for SH and 18 for IS.…”

“…The main MS/MS working parameters in the positive mode (1–4.7 min) were set as follows: 3.5 kV capillary voltage, 500 V nozzle voltage, 300°C gas temperature, 300°C sheath temperature, 12 L/min sheath gas flow, and 10 L/min gas flow. Quantification was performed via the multiple reaction monitoring of the transitions of m/z 330.2→239.1 and m/z 330.2→181.0 for SH 34 and m/z 289.0→97.1 for IS. Collision energy was set at 26 for SH and 18 for IS.…”

Zhengqing fengtongning sustained-release tablets prevents gout flares in the process of ULT

“…Sinomenine is an alkaloid derived from the Chinese herb sinomenium acutum, whose anti-inflammation effects have been widely evaluated and confirmed. Sinomenine has been used in a series of proprietary Chinese medicines for treating rheumatoid arthritis and other autoimmune diseases due to its anti-inflammatory and immunosuppressive properties [12] . Herein, we have designed a RCT protocol to compare the clinical efficacy of sinomenine and colchicine for prophylaxis of acute flares when initiating urate-lowering therapy.…”

Inhibitory effect of sinomenine on lung cancer cells via negative regulation of α7 nicotinic acetylcholine receptor

“…These preparations include sustained-release tablets, injections, and new preparations that can be used for intraarticular administration. [32][33][34][35][36] In clinical settings, SIN preparations are used for RA treatment with evident curative effects and no severe side effects. 37 In our previous study, we observed that the anti-inflammatory effects of SIN can be arrested by -BTX and 7…”

“…SIN, an alkaloid derived from a traditional Chinese herb that has been used for centuries in China, is consumed via different kinds of clinical preparations, such as Zhengqing Fengtongning tablets and injections. These preparations include sustained‐release tablets, injections, and new preparations that can be used for intra‐articular administration 32‐36 . In clinical settings, SIN preparations are used for RA treatment with evident curative effects and no severe side effects 37 .…”