An ultra-photostable and leakage-free fluorescent probe for long-term and high-fidelity mitochondria visualization and tracking

Rigidify styryl-pyridinium dyes to benzo[h]coumarin-based bright two-photon fluorescent probes for cellular bioimaging

“…[32][33][34] Our group has developed a number of uorescent mitochondrial probes based on the styryl-pyridinium platform with an ultrahigh signal-to-noise ratio and excellent leakage-free performance. [35][36][37][38] In this study, we designed and synthesized a uorescent probe for specically targeting mitochondria for a long time and successfully stained the nucleolus of xed cells. A comparative study of the photophysical properties and bioimaging performance of benzo[h]coumarinpyridinium (BS-CN) and a structurally similar styrylpyridinium (BS-MN) was carried out to determine the importance of structural rigidity for uorescence efficiency.…”