An ultra-sensitive monoclonal antibody-based fluorescent microsphere immunochromatographic test strip assay for detecting aflatoxin M 1 in milk

Highly Efficient Intramolecular Electrochemiluminescence Energy Transfer for Ultrasensitive Bioanalysis of Aflatoxin M1

“…ECL immunosensor 1-100 000 pg mL À1 0.3 pg mL À1 [ 26] Electrochemical aptasensor6 -60 pg mL À1 1.98 pg mL À1 [ 27] Immunosorbent assay0-1000pgmL À1 37.7 pg mL À1 [ 28] Electrochemical immunochip sensor 10-100pgmL À1 8pgmL À1 [ 29] Immunochromatographic strip test 10-320 pg mL À1 36.3 pg mL À1 [ 30] ECL aptasensor 0.05-100 pg mL À1 0.02 pg mL À1 This work Figure 6. A) Selectivityinvestigation of the proposedE CL aptasensorincubated with the target AFM1( 0.1 ng mL À1 )a nd other interference molecules: kanamycin (1.0 ng mL À1 ), glucose (1.0 ng mL À1 ), mixture (containing 0.1 ng mL À1 AFM1, 1.0 ng mL À1 kanamycin, 1.0 ng mL À1 glucose).…”

Development of a fluorescent immunoassay strip for the rapid quantitative detection of cadmium in rice

“…First, 400 µL of 0.05 M BB (pH = 8.0) in a 2-mL tube was mixed for 1 min with 100 µL of fluorescent microspheres. To activate the carboxyl groups on the surface of the microspheres (Zhang, Xu, et al, 2019;Yang et al, 2015;Zhang et al, 2016), we added 30 µL of aqueous EDC (10 mg/mL) dropwise. The solution was mixed at 37°C for 20 min and centrifuged at 13,000 rpm for 10 min at 10°C.…”

Production of antibodies and development of an enzyme-linked immunosorbent assay for 17β-estradiol in milk

“…The E 2 -CME hapten was conjugated to BSA and OVA to prepare the immunizing and coating conjugates using an active ester method (Wang et al, 2015;Zhang et al, 2016). Briefly, 33 mg E 2 -CME (0.1 mM), 95 mg EDC (0.5 mM), and 58 mg N-hydroxysuccinimide (0.5 mM) were dissolved in 2 mL of DMF and stirred at room temperature for 6 h. After centrifugation at 8000g for 10 min, the clear solution was added dropwise to 66 mg BSA (0.001 mM) or 45 mg OVA (0.001 mM) in 10 mL 0.01 M PBS at 4°C with gentle stirring overnight, followed by dialysis against PBS for 3 days and characterization by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)-MS. Hapten-to-protein molar ratios were calculated as follows (Peng et al, 2016):…”