An ultramicroelectrode for determination of intracellular oxygen. Light-irradiation-induced change in oxygen concentration in an algal protoplast

Matsue, Tomokazu; Koike, Shigeaki; Abe, Takayuki; Itabashi, Tetsuya; Uchida, Isamu

doi:10.1016/0167-4838(92)90468-s

Cited by 39 publications

(21 citation statements)

References 7 publications

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“…40,41 However, we have observed that the initial oxygen fluxes after illumination is of the same magnitude in both chloroplasts and thylakoid membranes. If the lack of terminal electron acceptors was responsible for controlling the oxygen generation rate, then one could reasonably have expected it to be significantly slower and less extensive in thylakoid membranes as compared to chloroplasts.…”

Section: Oxygen Evolution At Thylakoid Membrane Filmsmentioning

confidence: 73%

“…[23][24][25][26][27][28][29][30][31][32][33][34] Particularly relevant to this study are previous reports on aspects of photosynthesis, 35,36 for example, measurements of the local oxygen flux from single stomata of Brassica juncea 37 and the local oxygen flux above individual guard cells in intact plant leaves. 38 Two-dimensional imaging of the oxygen evolution from a single protoplast 39 (a plant cell with the cell wall removed) and quantification of the steady-state oxygen generation from single protoplasts [40][41][42] have also been reported. An individual component of the light-dependent photosynthetic pathway, photosystem I, has been investigated electrochemically 43 and using SECM.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Local Photosynthetic Flux Measurements at Isolated Chloroplasts and Thylakoid Membranes Using Scanning Electrochemical Microscopy (SECM)

et al. 2013

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Quantitative local photosynthetic flux measurements at isolated chloroplasts and thylakoid membranes using scanning electrochemical microscopy (SECM). Copies of full items can be used for personal research or study, educational, or not-forprofit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. Publisher's statement:This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry Part B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/jp403048fThe version presented here may differ from the published version or, version of record, if you wish to cite this item you are advised to consult the publisher's version. Please see the 'permanent WRAP url' above for details on accessing the published version and note that access may require a subscription. (CN) 6 4-), from the artificial electron acceptor ferricyanide (Fe(CN) 6 3-), using a stationary ultramicroelectrode at chloroplasts and thylakoid membranes (sourced from chloroplasts). Oxygen generation at films of chloroplasts and thylakoid membranes was detected directly during photosynthesis, but in the case of thylakoid membranes this switched to sustained oxygen consumption at longer illumination times. An initial oxygen concentration spike was detected over both chloroplast and thylakoid membrane films, and the kinetics of the oxygen generation were extracted by fitting the experimental data to a finite element method (FEM) simulation. In contrast to previous work, the oxygen generation spike was attributed to the limited size of the plastoquinone pool, a key component in the linear electron transport pathway and a contributing factor in photoinhibition. Finally, the mobile nature of the SECM probe, and its high spatial resolution, also allowed us to detect ferrocyanide produced from a single thylakoid membrane. These results further demonstrate the power of SECM for localized flux measurements in biological processes, in this case photosynthesis, and, that the high time resolution, combined with FEM simulations, allows the elucidation of quantitative kinetic information.

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Section: Oxygen Evolution At Thylakoid Membrane Filmsmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Quantitative Local Photosynthetic Flux Measurements at Isolated Chloroplasts and Thylakoid Membranes Using Scanning Electrochemical Microscopy (SECM)

et al. 2013

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“…A disk-type Pt electrode with a diameter of 20 μm (RG = 2, RG, the ratio of the insulating glass sheath radius to the disk electrode radius) was used as a SECM microelectrode probe. The fabrication of the Pt microelectrode has been described previously [28]. In brief, a fine Pt wire was inserted into a glass capillary and shielded by thermal fusing of the glass.…”

Section: Secm Set-upmentioning

confidence: 99%

Electrochemical monitoring of intracellular enzyme activity of single living mammalian cells by using a double-mediator system

Matsumae

Takahashi

Ino

et al. 2014

Analytica Chimica Acta

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“…The fabrication of the Pt microelectrode was described previously [29]. A Pt nanoelectrode was fabricated by simultaneously pulling a quartz capillary and a Pt wire inserted into the capillary under vacuum using a CO 2 laser puller P-2000 (Sutter) [30][31][32].…”

Section: Fabrication Of Pt Micro-and Nanoelectrodesmentioning

confidence: 99%

Influence of Tip Size on Single Yeast Cell Imaging Using Scanning Electrochemical Microscopy

et al. 2011

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The influence of the tip reaction on the substrate generation/tip collection mode SECM imaging of a single yeast cell was investigated by using various sizes of probe electrodes in mm-nm scales because the tip reaction would disturb the concentration profile of intracellular enzyme reactant formed around the cell. GC mode measurements of a single yeast cell were performed using a dual mediator system with lipophilic menadione and hydrophilic ferricyanide. We found downscaling of the tip size enabled quantitative SECM imaging of a single cell in GC mode without disturbing the concentration profile of the mediator formed around the cell by the tip reaction.

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An ultramicroelectrode for determination of intracellular oxygen. Light-irradiation-induced change in oxygen concentration in an algal protoplast

Cited by 39 publications

References 7 publications

Quantitative Local Photosynthetic Flux Measurements at Isolated Chloroplasts and Thylakoid Membranes Using Scanning Electrochemical Microscopy (SECM)

Quantitative Local Photosynthetic Flux Measurements at Isolated Chloroplasts and Thylakoid Membranes Using Scanning Electrochemical Microscopy (SECM)

Electrochemical monitoring of intracellular enzyme activity of single living mammalian cells by using a double-mediator system

Influence of Tip Size on Single Yeast Cell Imaging Using Scanning Electrochemical Microscopy

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