An ultrasensitive and selective electrochemical aptasensor based on rGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme

Rezaei, Behzad; Jamei, Hamid Reza; Ensafi, Ali A.

doi:10.1016/j.bios.2018.05.012

Cited by 87 publications

(18 citation statements)

References 36 publications

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“…MWCNTs were functionalized with the reported method in the literature . Briefly, 0.5 g MWCNTs was added to 100 mL of 5 mol L −1 nitric acid solution and refluxed for 16 h. The obtained solid product was washed with water for acid elimination and dried at room temperature.…”

Section: Methodsmentioning

confidence: 99%

MWCNTs/Ionic Liquid/Graphene Quantum Dots Nanocomposite Coated with Nickel‐Cobalt Bimetallic Catalyst as a Highly Selective Non‐enzymatic Sensor for Determination of Glucose

2018

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In this work, a glassy carbon electrode (GCE) was modified with multiwall carbon nanotubes/ionic liquid/graphene quantum dots (MWCNTs/IL/GQDs) nanocomposite. Then, the nanocomposite was decorated with nickel‐cobalt nanoparticles (Ni−Co NPs), and it was used as a non‐enzymatic glucose sensor. Field emission scanning electron microscopy, X‐ray diffraction spectroscopy, and energy dispersive spectroscopy were employed to prove the electrodeposition of the Ni−Co NPs on the surface of MWCNTs/IL/GQDs/GCE. Also, cyclic voltammetric and amperometric methods were utilized for the investigation of the electrochemical behaviour of the Ni−Co NPs/MWCNTs/IL/GQDs/GCE for glucose oxidation. The novel amperometric sensor displayed two linear ranges from 1.0 to 190.0 μmol L−1 and 190.0 to 4910 μmol L−1 with a low detection limit of 0.3 μmol L−1 as well as fast response time (2 s) and high stability. Also, the sensor showed good selectivity for glucose determination in the presence of ascorbic acid, citric acid, dopamine, uric acid, fructose, and sucrose, as potential interference species. Finally, the performance of the proposed sensor was investigated for the glucose determination in real samples. Ni−Co NPs/MWCNTs/IL/GQDs/GCE showed good sensitivity and excellent selectivity.

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Section: Methodsmentioning

confidence: 99%

MWCNTs/Ionic Liquid/Graphene Quantum Dots Nanocomposite Coated with Nickel‐Cobalt Bimetallic Catalyst as a Highly Selective Non‐enzymatic Sensor for Determination of Glucose

2018

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“…Deaminated chitin affinity chromatography is used for the isolation, purification and concentration of lysozyme from food or biological tissues [53][54]. Productions of chitin based biosensors seem promising [55]. Chitin films and nanoparticles are ideal supports in enzyme immobilization [56].…”

Section: Biotechnological Sectorsmentioning

confidence: 99%

Chitin – A Structural Polysaccharide for Health and Environmental Alimentation

Rahman¹,

Rahman²,

Shakil³

et al. 2021

BTBP

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Ever increasing physiological and environmental abnormalities warrant natural, safe, easy to extract, less expensive and eco-friendly bio-components of versatile usage. Chitin, the second most abundant polysaccharide in the world, seems promising in fighting diseases, boosting immunity up and maintaining environment fresh. Chitin is a polysaccharide of N-acetyl-D-glucosamine forms the exoskeletons of arthropods, mollusks and insects, cell walls of fungi and scales of fishes. Based on extraction, purification and integration enhancement, chitin could be of greater importance for the maintenance of human health, agriculture and environment. Present article compares and contrasts the methods utilized for extraction and purification of chitin from different sources and review its current fields and suggest the future aspects.

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“…There are other, very recent studies to detect lysozyme, but all of them either employ aptamers or work with non-biological samples, such as wine samples [24,25]. Other authors use nanorods [26] or carbon nanotubes, which are also functionalized with aptamers [27]. Lixiang Zuo does lysozyme detection in urine samples, but their method involves preparation of Mn-doped ZnS quantum dots [28] as does the one employed by Zhenli Qiu and coworkers for spiked serum samples [29].…”

Section: Introductionmentioning

confidence: 99%

Colorimetric, Naked-Eye Detection of Lysozyme in Human Urine with Gold Nanoparticles

et al. 2021

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The stabilizing effect of lysozymes to salt addition over a gold colloid are exploited in order to detect lysozymes in human urine samples. The present research is aimed at the development of a fast, naked-eye detection test for urinary lysozymuria, in which direct comparison with a colorimetric reference, allows for the immediate determination of positive/negative cases. CIEL*a*b* parameters were obtained from sample absorbance measurements, and their color difference with respect to a fixed reference point was measured by calculating the ΔE76 parameter, which is a measure of how well the colors can be distinguished by an untrained observer. Results show that a simple and quick test can reliably, in less than 15 min, give a positive colorimetric response in the naked eye for concentrations of a urinary lysozyme over 57.2 µg/mL. This concentration is well within the limits of that observed for leukemia-associated lysozymurias, among other disorders.

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An ultrasensitive and selective electrochemical aptasensor based on rGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme

Cited by 87 publications

References 36 publications

MWCNTs/Ionic Liquid/Graphene Quantum Dots Nanocomposite Coated with Nickel‐Cobalt Bimetallic Catalyst as a Highly Selective Non‐enzymatic Sensor for Determination of Glucose

MWCNTs/Ionic Liquid/Graphene Quantum Dots Nanocomposite Coated with Nickel‐Cobalt Bimetallic Catalyst as a Highly Selective Non‐enzymatic Sensor for Determination of Glucose

Chitin – A Structural Polysaccharide for Health and Environmental Alimentation

Colorimetric, Naked-Eye Detection of Lysozyme in Human Urine with Gold Nanoparticles

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